We narrowed to 13,381 results for: SHI

1. pLenti-U6-tdTomato-P2A-BlasR (LRT2B)

   Plasmid

   #110854

   Purpose

   Lentiviral vector for constitutive expression of sgRNAs; includes tdTomato and BlasticidinS resistance markers

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   sgRNA scaffold with spacer
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   U6

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. lentiCRISPR v2-sgControl

   Plasmid

   #125836

   Purpose

   KO control

   Depositor

   Boyi Gan

   Article

   Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

   Insert

   Control
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-HaloAKAR2.2-T2A-EGFP

   Plasmid

   #216490

   Purpose

   Expression of chemigenetic PKA biosensor (low basal brightness, extremly high dynamic range) and EGFP transfection marker in mammalian cells

   Depositor

   Jin Zhang

   Article

   Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

   Insert

   HaloAKAR2.2
   

   Use

   Tags

   T2A-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA 3.3 LRP5

   Plasmid

   #115907

   Purpose

   Expresses human LRP5 in mammalian cells.

   Depositor

   Harald Junge

   Article

   Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

   Insert

   LRP5 (LRP5 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA 3.3 HA-hTSPAN12

   Plasmid

   #115784

   Purpose

   Expresses human TSPAN12 with N-terminal HA-tag in mammalian cells. Contains multiple silent mutations that introduce restriction sites.

   Depositor

   Harald Junge

   Article

   Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

   Insert

   TSPAN12 (TSPAN12 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   multiple silent mutations introduce restriction s…

   Promoter

   CMV

   Available since

   Oct. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX-EZH2

   Plasmid

   #28060

   Depositor

   Mien-Chie Hung

   Article

   Wei et al Nat Cell Biol. 2011 Jan . 13(1):87-94.

   Insert

   EZH2 (EZH2 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-ABERA-P2A-Puro

   Plasmid

   #112675

   Purpose

   Lentivirus for constitutive expression of ABE7.10 RA (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   ABERA
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   D10A

   Promoter

   EF1s

   Available since

   July 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pphiLOV3-N1

   Plasmid

   #178973

   Purpose

   Expresses phiLOV3 in mammalian cells

   Depositor

   Kiryl Piatkevich

   Article

   Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

   Insert

   phiLOV3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCS2plus-mKate2

   Plasmid

   #162624

   Purpose

   Allows for transcription of mKate2 control for injection into zebrafish embryos for BiFC assays

   Depositor

   Marco Morsch

   Article

   Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

   Insert

   mKate2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SP6

   Available since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. ATG-2460

    Plasmid

    #108713

    Purpose

    EF1-CBR2opt-T2A-copGFP (construct used for lentiviral transduction)

    Depositor

    Keith Wood

    Article

    Hall et al Nat Commun. 2018 Jan 9;9(1):132. doi: 10

    Insert

    CBR2opt-copGFP fusion
    

    Use

    Lentiviral

    Tags

    CBR2opt fused to copGFP

    Expression

    Mutation

    Promega used directed evolution to create CBR (Cl…

    Promoter

    elongation factor-1

    Available since

    Oct. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-Cas9-P2A-Puro

    Plasmid

    #110837

    Purpose

    Lentiviral vector for expression of Cas9 in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-HaloEKAR2.2-T2A-EGFP

    Plasmid

    #216495

    Purpose

    Expression of chemigenetic ERK biosensor (low basal brightness, extremly high dynamic range) and EGFP transfection marker in mammalian cells

    Depositor

    Jin Zhang

    Article

    Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

    Insert

    HaloEKAR2.2
    

    Use

    Tags

    T2A-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJMP1

    Plasmid

    #79873

    Purpose

    Bacillus subtilis dCas9 expression vector; integrates into lacA/ganA

    Depositor

    Stanley Qi, Carol Gross, Jason Peters

    Article

    Peters et al Cell. 2016 Jun 2;165(6):1493-506. doi: 1

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    xylA

    Available since

    Oct. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX330-SpCas9-NG

    Plasmid

    #117919

    Purpose

    Expresses 3xFLAG-NLS-SpCas9-NG-NLS in mammalian cells.

    Depositor

    Osamu Nureki

    Article

    Nishimasu et al Science. 2018 Aug 30. pii: science.aas91

    Insert

    SpCas9-NG (L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-Myc-NLRP3(R260W)

    Plasmid

    #73956

    Purpose

    Express c-Myc-tagged human NLRP3(R260W) in mammalian cells

    Depositor

    Christian Stehlik

    Article

    Dorfleutner et al Infect Immun. 2007 Mar;75(3):1484-92. Ep

    Insert

    NLR family, pyrin domain containing 3 (NLRP3) (NLRP3 Human)
    

    Use

    Tags

    c-myc

    Expression

    Mammalian

    Mutation

    R260W

    Promoter

    CMV iE

    Available since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pJMP3

    Plasmid

    #79875

    Purpose

    Bacillus subtilis sgRNA expression vector; integrates into thrC

    Depositor

    Stanley Qi, Carol Gross, Jason Peters

    Article

    Peters et al Cell. 2016 Jun 2;165(6):1493-506. doi: 1

    Insert

    sgRNA RR1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    veg

    Available since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pNIC28-DENV3-NS5FL

    Plasmid

    #86013

    Purpose

    Expression of dengue virus 3 NS5 protein

    Depositor

    Dahai Luo

    Article

    Zhao et al PLoS Pathog. 2015 Mar 16;11(3):e1004682.

    Insert

    DENV3-NS5FL
    

    Use

    Tags

    hexa-histidine tag

    Expression

    Bacterial

    Mutation

    amino acid residues 6 to 895 of DENV3 NS5

    Promoter

    T7

    Available since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. plentiCMV-sct-pp65-b2m-HLA-A0201

    Plasmid

    #196511

    Purpose

    Express single-chain peptide-MHC (pp65 CMV epitope on HLA-A0201)

    Depositor

    Howard Chang

    Article

    Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

    Insert

    single chain of a pp65 peptide, b2m and HLA A0201
    

    Use

    Lentiviral

    Tags

    NA

    Expression

    Mammalian

    Mutation

    Y84C

    Promoter

    CMV

    Available since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. Lenti_SaCRISPR_GFP

    Plasmid

    #118636

    Purpose

    To clone sgRNA for SaCas9 (SauCas9)

    Depositor

    Christopher Vakoc

    Article

    Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-Flag-mRIPK1-S321E

    Plasmid

    #115340

    Purpose

    Mammalian expression of Flag-mRIPK1-S321E

    Depositor

    Junying Yuan

    Article

    Geng et al Nat Commun. 2017 Aug 25;8(1):359. doi: 1

    Insert

    RIPK1 (Ripk1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S321E

    Promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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