-
Plasmid#15150DepositorInsertCHX10 3'UTR (VSX2 Chicken)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 8, 2007AvailabilityAcademic Institutions and Nonprofits only -
pVEG17*
Plasmid#111856PurposeXylose-responsive Gal3p-Syn4.1 under control of native GAL1p (promoter)DepositorInsertGAL1p-GAL3-Syn4.1-TEFt
UseSynthetic BiologyTagsExpressionYeastMutationD68N, V69M, A109V, I271LPromoterGAL1pAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVEG16*
Plasmid#111855PurposeXylose-responsive Gal3p-Syn4.1 under control of native GAL3p (promoter).DepositorInsertGAL3p-GAL3-Syn4.1-TEFt
UseSynthetic BiologyTagsExpressionYeastMutationD68N, V69M, A109V, I271LPromoterGAL3Available sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV Synapsin Intron H2B Gcamp 6f wpre Pzac2.1
Plasmid#74144PurposeCalcium sensorDepositorInsertGCaMP6f
UseAAVTagsH2BExpressionMutationPromotersynapsinAvailable sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pOmnibac_ZZ_linker_K963_GFP_SNAP
Plasmid#159727PurposeExpresses kinesin-1 (amino acids 1-963) in Sf9 cells with a C-terminal GFP and SNAP tagDepositorInsertKIF5B (full length, 1-963 amino acids), K963 (KIF5B Human)
UseTagsGFP and SNAP tags and ZZ affinity tagExpressionInsectMutationPromoterAvailable sinceJan. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOmnibac_ZZ_linker_ybbR_MAP7_N
Plasmid#159728PurposeExpresses MAP7 (amino acids 1-316) in Sf9 cells with a ZZ affinity tag and a ybbR tagDepositorInsertMAP7 (only amino acids 1-316)(includes MTBD and P123 domains) (MAP7 Human)
UseTagsZZ affinity tag and ybbR tagExpressionInsectMutationPromoterAvailable sinceJan. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV Synapsin Intron H2B Gcamp 6s wpre Pzac2.1
Plasmid#74150Purposecalcium sensorDepositorInsertGCaMP6s
UseAAVTagsH2BExpressionMutationPromoterSynapsinAvailable sinceApril 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
NLS-HA-2xMCP-Ezh2
Plasmid#126590PurposeExpresses MCP (MS2 Coat Protein) fusion to Ezh2 in mammalian cells, lentiviral backboneDepositorInsert2XMCP-Ezh2 (Ezh2 Mouse, Synthetic)
UseLentiviralTagsNLS-HAExpressionMammalianMutationPromoterhuman ubiquitin C promoterAvailable sinceMay 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-PK
Plasmid#24163DepositorInsertpyruvate kinase
UseTagsGFPExpressionMammalianMutationn/aPromoterAvailable sinceMarch 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
-
AAV-hSyn-RiboL1-jGCaMP8s
Plasmid#169247PurposeAAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8s, with a flexible GS-linker sequence attaching the ribosomal tag to the jGCaMP8s protein.DepositorInsertRiboL1-jGCaMP8s
UseAAVTags6xHisExpressionMammalianMutationPromoterhSynAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-RiboL1-jGCaMP8m
Plasmid#169248PurposeAAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8m, with a flexible GS-linker sequence attaching the ribosomal tag to the jGCaMP8m protein.DepositorInsertRiboL1-jGCaMP8m
UseAAVTags6xHisExpressionMammalianMutationPromoterhSynAvailable sinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-RiboL1-jGCaMP8f
Plasmid#169249PurposeAAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8f, with a flexible GS-linker sequence attaching the ribosomal tag to the jGCaMP8f protein.DepositorInsertRiboL1-jGCaMP8f
UseAAVTags6xHisExpressionMammalianMutationPromoterhSynAvailable sinceDec. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
phCMV-IE1
Plasmid#118048PurposeGBPart - Cytomegalovirus enhancer and promoter for constitutive high level expression in mammalian cellsDepositorInsertCytomegalovirus enhancer and promoter
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-Ribo-jGCaMP8s
Plasmid#167572PurposeDEPRECATED: Please see Addgene item #169247 for an improved version of this plasmid. AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8s.DepositorHas ServiceAAV1InsertRibo-GCaMP8s
UseAAVTags6xHisExpressionMammalianMutationPromoterhSynAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-Ribo-jGCaMP8m
Plasmid#167574PurposeDEPRECATED: Please see Addgene item #169248 for an improved version of this plasmid. AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8m.DepositorHas ServiceAAV1InsertRibo-GCaMP8m
UseAAVTags6xHisExpressionMammalianMutationPromoterhSynAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-Ribo-jGCaMP8f
Plasmid#167573PurposeDEPRECATED: Please see Addgene item #169249 (coming soon) for an improved version of this plasmid. AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8f.DepositorInsertRibo-GCaMP8f
UseAAVTags6xHisExpressionMammalianMutationPromoterhSynAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCIG-FGFRdn
Plasmid#80431PurposeExpression of a dominant-negative FGFR1 with a nuclear EGFP reporter in chick embryosDepositorInsertFibroblast growth factor receptor 1 (FGFR1 Chicken)
UseTagsEGFPExpressionMammalianMutationcontains aa 1–425Promoterbeta-actin promoterAvailable sinceFeb. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
BDS-2 3x WT (p53 binding site)
Plasmid#16515DepositorInsertp53 binding site (SFN Human)
UseTagsLuciferaseExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
FLAG-KIF5A delta Tail
Plasmid#166955PurposeExpresses FLAG-tagged KIF5A protein (1-820aa) in pcDNA3.1DepositorInsertKIF5A (Kif5a Mouse)
UseTagsFLAGExpressionMammalianMutationKIF5A (1-820aa)PromoterCMVAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only