We narrowed to 1,212 results for: ncl

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pTSA-13

Plasmid

#17327

Depositor

Takeshi Sano

Article

Sano et al J Biol Chem. 1995 Nov 24. 270(47):28204-

Insert

streptavidin

Expression

Bacterial

Mutation

Truncated to include only amino acids 16-133 of m…

Available Since

Feb. 25, 2008

Availability

Academic Institutions and Nonprofits only

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MET-HYGRO

Plasmid

#218113

Purpose

Integration backbone for the MET15 integration site of S. cerevisiae. Includes a HygromycinR gene and a sfGFP drop-out.

Depositor

Carl Denard

Article

Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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LYS-Leu2

Plasmid

#218114

Purpose

Integration backbone for the LYS2 integration site of S. cerevisiae. Includes a Leu2 nutritional marker and a sfGFP drop-out.

Depositor

Carl Denard

Article

Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pET303-colE1T

Plasmid

#155180

Purpose

Expresses colicin E1 residues 1-190

Depositor

Joanna Slusky

Article

Budiardjo et al Elife. 2022 Feb 24;11. pii: 73297. doi:

Insert

colicin E1 residues 1-190

Tags

C-terminal 6x Histidine tag

Expression

Bacterial

Mutation

truncation of colicin E1 including 1-190

Promoter

T7

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pT2SK

Plasmid

#59383

Purpose

Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a kanamycin resistance gene.

Depositor

Shelley Copley

Article

Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

Insert

TT-ISceI-kan

Use

Template

Available Since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only

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p3E-APEX2-P2A-EGFP

Plasmid

#108896

Purpose

Multisite gateway vector for 3' tagging with APEX2 (includes cytoplasmic GFP reporter)

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

APEX2-P2A-EGFP

Use

Multisite gateway

Available Since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pBS_KS_attB2_SA(1)_T2ALexA

Plasmid

#125221

Purpose

Artificial phase 1 exon to include a spliced T2A-LexA effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

Depositor

Herman Dierick

Article

Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

Insert

LexA

Use

Other

Tags

T2A

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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pBS_KS_attB2_SA(0)_T2ALexA

Plasmid

#125220

Purpose

Artificial phase 0 exon to include a spliced T2A-LexA effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

Depositor

Herman Dierick

Article

Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

Insert

LexA

Use

Other

Tags

T2A

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-PRSX8 eArchT3.0-EYFP

Plasmid

#89538

Purpose

Expresses eArchT 3.0-eYFP in Phox2 (including catecholamine) neurons

Depositor

Ruth Stornetta

Article

Burke et al J Physiol. 2015 Jul 1;593(13):2909-26. d

Insert

eArchT 3.0

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Promoter

PRSx8

Available Since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pBS_KS_attB2_SA(2)_T2ALexA

Plasmid

#125222

Purpose

Artificial phase 2 exon to include a spliced T2A-LexA effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

Depositor

Herman Dierick

Article

Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

Insert

LexA

Use

Other

Tags

T2A

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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Ac5-STABLE1-blast

Plasmid

#208033

Purpose

Enables expression of an ORF (with or without tags) and selection with blasticidin in insect cells. Includes GFP-T2A-blast with Actin5C promoter.

Depositor

Rosa Barrio, James Sutherland

Article

Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep000

Type

Empty backbone

Expression

Insect

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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Cdk9-Halo donor

Plasmid

#113113

Purpose

Donor plasmid for knock-in HaloTag fusing to the c-terminal of mouse Cdk9 coding sequence

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

Cdk9 donor region including homology arms and HaloTag

Use

Synthetic Biology

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pECFP-DEVG-Venus

Plasmid

#24538

Depositor

Peter Sorger

Article

Albeck et al PLoS Biol. 2008 Dec 2. 6(12):2831-52.

Insert

ECFP-DEVG-Venus

Tags

ECFP and Venus

Expression

Mammalian

Mutation

DEVDR mutated to DEVG

Promoter

CMV

Available Since

June 11, 2010

Availability

Academic Institutions and Nonprofits only
pXF276

Plasmid

#139048

Purpose

MosSCI SapTrap assembly: Cassette 3B for 5-cassette system

Depositor

Erik Griffin

Article

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

lov domain (includes STOP codon)

Expression

Worm

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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Fus-BcLOV-mCh

Plasmid

#208277

Purpose

Expresses Fus-BcLOV fusion for optogenetic membrane recruitment. Includes an mCherry tag.

Depositor

Lukasz Bugaj

Article

Pal et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

Insert

Fus-BcLOV-mCh

Expression

Mammalian

Promoter

pCMV

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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Gata4-GFP donor

Plasmid

#113115

Purpose

Donor plasmid for knock-in eGFP fusing to the c-terminal of mouse Gata4 coding sequence

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

Gata4 donor region including homology arms and eGFP

Use

Synthetic Biology

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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RAB14 S25N

Plasmid

#61495

Purpose

to overexpress dominant negative RAB14. Vector includes GFP driven by hUbC promoter

Depositor

Curt Civin

Article

Kim et al Br J Haematol. 2014 Oct 14. doi: 10.1111

Insert

RAB14 (RAB14 Human)

Use

Lentiviral

Mutation

S25N, inactive form

Promoter

EF1a

Available Since

Jan. 6, 2015

Availability

Academic Institutions and Nonprofits only
pSDH52

Plasmid

#139049

Purpose

MosSCI SapTrap assembly: Cassette 3B for 5-cassette system

Depositor

Erik Griffin

Article

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

epdz (includes STOP codon)

Expression

Worm

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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RAB14 Q70L

Plasmid

#61494

Purpose

to overexpress constitutively active RAB14. Vector includes GFP driven by hUbC promoter

Depositor

Curt Civin

Article

Kim et al Br J Haematol. 2014 Oct 14. doi: 10.1111

Insert

RAB14 (RAB14 Human)

Use

Lentiviral

Mutation

Q70L, active form

Promoter

EF1a

Available Since

Jan. 6, 2015

Availability

Academic Institutions and Nonprofits only
1455_pDEST_miniTol2_R4-R2_MCS

Plasmid

#171794

Purpose

pDEST miniTol2-clone for R4-R2 3-component gateway assembly (p5E + pME). Include a Multiple Cloning Site for selection marker insertion.

Depositor

Jean Giacomotto

Article

Tromp et al Front Physiol. 2023 Aug 2;14:1221310. do

Type

Empty backbone

Available Since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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