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pUdO4t

Plasmid

#210249

Purpose

low-copy plasmid for bacterial expression

Depositor

François-Xavier Campbell-Valois

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Type

Empty backbone

Expression

Bacterial

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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pJUMP18-cueR_O

Plasmid

#127026

Purpose

Basic Part O- ORF; cueR; transcription factor

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part cueR_o

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP43-2A(sfGFP)

Plasmid

#126987

Purpose

Level 2 vector. sfGFP reporter. Origin pBBR1 (medium-copy; broad-host-range)

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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pBlueRIP.cOVA

Plasmid

#69595

Purpose

contains cytoplasmic form of ovalbumin (OVA) cDNA in pBlueRIP

Depositor

Francis R. Carbone

Article

Kurts et al J Exp Med. 1996 Sep 1;184(3):923-30.

Insert

OVA (OVAL Chicken)

Expression

Mammalian

Promoter

rat insulin promoter (RIP)

Available Since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

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pMT-Mad2-GFP

Plasmid

#24284

Depositor

Ron Vale

Article

Griffis et al J Cell Biol. 2007 Jun 18. 177(6):1005-15

Insert

Mad2 (mad2 Fly)

Tags

GFP

Expression

Insect

Available Since

May 10, 2010

Availability

Academic Institutions and Nonprofits only
pRS424-TUB1-Cterminal6xHis

Plasmid

#60387

Purpose

Expression of yeast alpha tubulin (TUB1)- Cterminal 6xHis using a GAL promoter

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB1 (TUB1 Budding Yeast)

Tags

6xHIS

Expression

Yeast

Promoter

GAL

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
pBlueRIP.OVA

Plasmid

#69597

Purpose

contains soluble form of ovalbumin (OVA) cDNA in pBlueRIP (used to generate RIP-OVAhi and RIP-OVA lo mice)

Depositor

Francis R. Carbone

Article

Blanas et al Science. 1996 Dec 6;274(5293):1707-9.

Insert

OVA (OVAL Chicken)

Expression

Mammalian

Promoter

rat insulin promoter (RIP)

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pJUMP19-Ptac_P

Plasmid

#127004

Purpose

Basic Part P- Promoter (non-constitutive); Ptac; IPTG/lactose induced; controlled by LacI.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part Ptac_P

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pIS1-miR-206 site

Plasmid

#25027

Depositor

Bob Weinberg

Article

Valastyan et al Cell. 2009 Jun 12. 137(6):1032-46.

Insert

synthetic miR-206 site

Use

Luciferase

Expression

Mammalian

Available Since

June 18, 2010

Availability

Academic Institutions and Nonprofits only
pMT-GFP-SAK

Plasmid

#31755

Depositor

Ron Vale

Article

Goodwin et al Cell. 2010 Oct 15;143(2):263-74.

Insert

Sak (SAK Fly)

Tags

GFP

Expression

Insect

Mutation

A115S

Promoter

Metallothionein

Available Since

Jan. 20, 2012

Availability

Academic Institutions and Nonprofits only
pMT-GFP-Sas4

Plasmid

#31756

Depositor

Ron Vale

Article

Goodwin et al Cell. 2010 Oct 15;143(2):263-74.

Insert

Sas4 (Sas-4 Fly)

Tags

GFP

Expression

Insect

Mutation

N70T

Promoter

Metallothionein

Available Since

Jan. 20, 2012

Availability

Academic Institutions and Nonprofits only
pMT-GFP-Pat coiled-coil (cc)

Plasmid

#31757

Depositor

Ron Vale

Article

Goodwin et al Cell. 2010 Oct 15;143(2):263-74.

Insert

Patronin (Patronin Fly)

Tags

GFP

Expression

Insect

Mutation

Contains only the coiled-coil domain: aa 535-1457

Promoter

Metallothionein

Available Since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only
413-Met25 Kar2 TorsinADE GFP

Plasmid

#32120

Depositor

Susan Lindquist

Article

Valastyan et al PLoS One. 2011;6(7):e22744. Epub 2011 Ju

Insert

TorsinA (TOR1A Human)

Tags

GFP and Kar2

Expression

Yeast

Mutation

deletion of E at 302 or 303

Available Since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only
pJUMP19-J23102_P

Plasmid

#126997

Purpose

Basic Part P- Promoter (constitutive); J23102.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part J23102_P

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP39-1A(sfGFP)

Plasmid

#126983

Purpose

Level 1 vector with alternative selection marker (chloramphenicol). OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP27ts-1A(lacZ)

Plasmid

#126977

Purpose

Level 1 vector. With lacZ as alternative cloning reporter. Thermosensitive origin pSC101 (low copy number)

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

RepA A56V thermosensitive mutation

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pPS1601

Plasmid

#8921

Depositor

Pamela Silver

Article

Valentini et al Genetics. 2002 Feb . 160(2):393-405.

Insert

PAB1 (PAB1 Budding Yeast)

Expression

Yeast

Available Since

Aug. 19, 2005

Availability

Academic Institutions and Nonprofits only
pIS1-miR-335 site

Plasmid

#25028

Depositor

Bob Weinberg

Article

Valastyan et al Cell. 2009 Jun 12. 137(6):1032-46.

Insert

synthetic miR-335 site

Use

Luciferase

Expression

Mammalian

Available Since

June 18, 2010

Availability

Academic Institutions and Nonprofits only
His10-PS-SNAPf-9E (XSB526)

Plasmid

#78514

Purpose

Construct for expressing His10 - SNAPf -9E in E. Coli

Depositor

Ron Vale

Article

Su et al Science. 2016 Apr 29;352(6285):595-9. do

Insert

SNAPf

Tags

His10 followed by a prescission protease claevage…

Expression

Bacterial

Mutation

9E added to the C-terminal of SNAPf

Available Since

June 6, 2016

Availability

Academic Institutions and Nonprofits only

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pJUMP18-Streptag_C

Plasmid

#127036

Purpose

Basic Part C- C-terminus; Strep-tag II

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part StrepII_tag_C

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

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