162,109 results

1. pCRISPR-PE-bacteria

   Plasmid

   #172715

   Purpose

   Expression of an E. coli codon optimized fusion protein of Cas9n-linker-M-MLV2 for prime editing

   Depositor

   Tilmann Weber

   Article

   Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

   Insert

   Cas9n-linker-M-MLV2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   H840A of Cas9

   Promoter

   Available since

   Sept. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pICH41421

   Plasmid

   #50339

   Depositor

   Nicola Patron, Sylvestre Marillonnet

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   3'UTR, polyadenylation signal/terminator, nos (A. tumefaciens)
   

   Use

   Plant expression

   Tags

   Expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Promoter

   Available since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHIV-iRFP720-E2A-Luc

   Plasmid

   #104587

   Purpose

   Lentivirus vector for bi-cistronic expression of iRFP720 and firefly luciferase from EF1a promoter

   Depositor

   Antonius Plagge

   Article

   Comenge et al Elife. 2018 Jun 27;7. pii: 33140. doi: 1

   Insert

   iRFP720-E2A-Luciferase
   

   Use

   Lentiviral and Luciferase

   Tags

   bi-cistronic

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pWPXLd

   Plasmid

   #12258

   Depositor

   Didier Trono

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRwB

   Plasmid

   #48734

   Purpose

   dsRed plasmid

   Depositor

   John Schiller

   Article

   Johnson et al J Virol. 2009 Mar;83(5):2067-74. Epub 20

   Insert

   dsRed-Express
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   March 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. 1481 pcDNA3 flag HA 14-3-3 zeta

   Plasmid

   #9002

   Depositor

   William Sellers

   Insert

   14-3-3 zeta (YWHAZ Bovine)
   

   Use

   Tags

   HA and flag

   Expression

   Mammalian

   Mutation

   T226A

   Promoter

   Available since

   April 20, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTP943

   Plasmid

   #104157

   Purpose

   Bacterial expression plasmid of anti-mouse IgG1 Fab nanobody TP886 (3x Cysteines)

   Depositor

   Dirk Görlich

   Article

   Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

   Insert

   Anti-mouse IgG1 Fab specific nanobody TP886
   

   Use

   Tags

   14xHistidine tag and NEDD8 from Brachypodium dist…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV-CaMKIIa-ChRmine-mScarlet-WPRE

   Plasmid

   #130990

   Purpose

   AAV vector to drive the expression of ChRmine-mScarlet under the control of CamKIIa promoter

   Depositor

   Karl Deisseroth

   Article

   Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

   Insert

   ChRmine
   

   Use

   AAV

   Tags

   mScarlet

   Expression

   Mutation

   Promoter

   CaMKIIa

   Available since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. EGFP-PIP5K1A

   Plasmid

   #202720

   Purpose

   expresses fluorescent protein-tagged enzyme

   Depositor

   Gerry Hammond

   Article

   Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

   Insert

   EGFP:PIP5K1A (PIP5K1A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. perbB2-EGFP

    Plasmid

    #39321

    Depositor

    Martin Offterdinger

    Article

    Offterdinger et al Traffic. 2008 Jan;9(1):147-55. Epub 2007

    Insert

    erbB2 (ERBB2 Human)
    

    Use

    Tags

    egfp

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. COM-epegRNA-Dnmt1

    Plasmid

    #211376

    Purpose

    Dnmt1 targeting epegRNA with COM insertion in gRNA scaffold for v3b PE-eVLP production

    Depositor

    David Liu

    Article

    An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

    Insert

    COM-epegRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. C098

    Plasmid

    #174345

    Purpose

    Template for making Nluc mRNA reporter with pA139.

    Depositor

    David Bartel

    Article

    Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

    Insert

    NanoLuc luciferase
    

    Use

    In vitro transcription template

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBS U6a gRNA

    Plasmid

    #158961

    Purpose

    gRNA backbone driven by zebrafish U6a promoter

    Depositor

    Michael Brand

    Article

    Hans et al Nat Commun. 2021 Feb 18;12(1):1125. doi:

    Inserts

    zf U6a promoter
    gRNA backbone
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pHR-CMV-TetO2_mRuby2

    Plasmid

    #113894

    Purpose

    Mammalian (inducible) expression of cytosolic mRuby2

    Depositor

    A. Radu Aricescu

    Article

    Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

    Insert

    monomeric Ruby2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV-MIE-TetO2

    Available since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CMV-LAMP1-HaloTag

    Plasmid

    #164209

    Purpose

    Expresses HaloTag tagged LAMP1 under CMV promoter

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

    Insert

    LAMP1 (Lamp1 Rat)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBABE_hygro_mRFP1_NRF2

    Plasmid

    #136579

    Purpose

    mammalian expression of mRFP1-tagged human NRF2

    Depositor

    Kevin Janes

    Article

    Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

    Insert

    NRF2 (NFE2L2 Human)
    

    Use

    Retroviral

    Tags

    N-mRFP1-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-CW3SL-EGFP

    Plasmid

    #61463

    Purpose

    AAV vector with CaMKIIa promoter, EGFP, and W3SL(shorten expression cassette with relatively high expression to maximize expression of larger genes)

    Depositor

    Bong-Kiun Kaang

    Article

    Choi et al Mol Brain. 2014 Mar 11;7:17. doi: 10.118

    Insert

    EGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CaMKIIa

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. 1479 pcDNA3 flag HA 14-3-3 gamma

    Plasmid

    #9000

    Depositor

    William Sellers

    Insert

    14-3-3 gamma (Ywhag Rat)
    

    Use

    Tags

    HA and flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 14, 2005

    Availability

    Academic Institutions and Nonprofits only

19. hu6 HGPS sgRNA expression and ABE7.10max VRQR C terminal AAV vector

    Plasmid

    #154430

    Purpose

    hu6 HGPS sgRNA expression and ABE7.10max VRQR C terminal AAV vector

    Depositor

    David Liu

    Article

    Koblan et al Nature. 2021 Jan;589(7843):608-614. doi:

    Insert

    hu6 HGPS sgRNA expression and ABE7.10max VRQR C terminal AAV vector
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    VRQR point mutations in SpCas9

    Promoter

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHCMV_VSBV_G

    Plasmid

    #207312

    Purpose

    Mammalian expression of Variegated Squirrel Bornavirus G protein

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    VSBV_G
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mammalian codon optimization

    Promoter

    CMV promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge