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SpyCatcher002-oHept

Plasmid

#124671

Purpose

Expresses SpyCatcher002-oHept for heptamerization of SpyTag-proteins via heptameric coiled-coil

Depositor

Mark Howarth

Article

Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

Insert

SpyCatcher002-oHept

Tags

C-tag, His6, and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pLCKO-CMV-Blasticidin-P2A-3xHA-TMEM106B_Human

Plasmid

#238130

Purpose

This plasmid encodes the full-length human TMEM106B protein fused with a 3xHA tag, along with an N-terminal blasticidin selection marker.

Depositor

Dirk Daelemans

Article

Baggen et al Cell. 2023 Aug 3;186(16):3427-3442.e22.

Insert

TMEM106B (TMEM106B Human)

Use

Lentiviral

Tags

Blasticidin-P2A-3xHA

Expression

Mammalian

Promoter

CMV

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pSLIK CA ROCK2

Plasmid

#84649

Purpose

Lentiviral expression of doxycycline-inducible constitutively active ROCK2 and co-expression of Venus

Depositor

Sanjay Kumar

Article

Wong et al Cancer Res. 2015 Mar 15;75(6):1113-22. d

Insert

ROCK2 (ROCK2 Bovine)

Use

Lentiviral

Expression

Mammalian

Mutation

Constitutive Active (please see depositor comment…

Available Since

Dec. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1 hSCN5A

Plasmid

#145374

Purpose

expresses human Nav1.5 (WT) in mammalian cells

Depositor

Hugues Abriel, Robert Kass

Article

Rougier et al Am J Physiol Cell Physiol. 2005 Mar;288(

Insert

sodium voltage-gated channel alpha subunit 5 (SCN5A Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pLentiV2-dCas9-VP64

Plasmid

#141104

Purpose

Expression of gRNA and dCas9-VP64 from the same plasmid for CRISPRa

Depositor

Igor Ulitsky

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

June 1, 2020

Availability

Academic Institutions and Nonprofits only

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mPlum-N1

Plasmid

#54629

Purpose

Localization: N1 Cloning Vector, Excitation: 590, Emission: 649

Depositor

Michael Davidson

Article

Kremers et al Nat Methods. 2009 May;6(5):355-8. doi: 1

Type

Empty backbone

Tags

mPlum

Expression

Mammalian

Available Since

June 12, 2014

Availability

Academic Institutions and Nonprofits only

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pLOVE-miR302/367

Plasmid

#31310

Depositor

Ed Morrisey

Article

Anokye-Danso et al Cell Stem Cell. 2011 Apr 8. 8(4):376-88.

Insert

mmu-miR302/367

Use

Lentiviral

Expression

Mammalian

Mutation

None

Available Since

Oct. 25, 2011

Availability

Academic Institutions and Nonprofits only
AiP5001 RabV-CVS-N2cdeltaG-histone-EGFP

Plasmid

#176283

Purpose

Recombinant rabies virus expressing histone-EGFP

Depositor

Allen Institute for Brain Science, Shenqin Yao

Article

Yao et al Nat Neurosci. 2023 Feb;26(2):350-364. do

Insert

H2B (H2BC11 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pLX304_CMV::mCherry-LgBiT-FLAG-NES

Plasmid

#199715

Purpose

Expresses LgBiT, fused to mCherry, in the cytosol of mammalian cells. Can be used in generating stable cell lines via lentiviral transduction.

Depositor

Alice Ting

Article

Roh et al J Am Chem Soc. 2023 May 10;145(18):10220

Insert

mCherry-LgBiT-FLAG-NES

Use

Lentiviral and Luciferase

Expression

Mammalian

Promoter

CMV

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA4/Full length FVIII

Plasmid

#41036

Depositor

Robert Peters

Article

Peters et al J Thromb Haemost. 2012 Dec 4. doi: 10.11

Insert

FVIII (F8 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only

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pUC21

Plasmid

#49787

Purpose

E. coli cloning vector (AmpR, high copy, blue/white selection, M13 IR)

Depositor

Joachim Messing

Article

Vieira et al Gene. 1991 Apr;100:189-94.

Type

Empty backbone

Use

Cloning vector

Available Since

July 3, 2014

Availability

Academic Institutions and Nonprofits only

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pFastBac His6 MBP TEV cloning vector with BioBrick polycistronic restriction sites (11C)

Plasmid

#48296

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Tags

His6 and MBP

Expression

Insect

Available Since

Sept. 23, 2013

Availability

Academic Institutions and Nonprofits only

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ERBB2 gRNA (BRDN0001148320)

Plasmid

#76378

Purpose

3rd generation lentiviral gRNA plasmid targeting human ERBB2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ERBB2 (ERBB2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-fDIO hChR2(H134R)-EYFP

Plasmid

#55639

Purpose

Flp-Dependent ChR2-EYFP

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Has Service

AAV Retrograde

Insert

hChR2(H134R)-EYFP

Use

AAV

Tags

EYFP

Expression

Mammalian

Promoter

Ef1a

Available Since

Aug. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pBPHLWL-LexA

Plasmid

#26258

Depositor

Tom Clandinin

Article

Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

Insert

LexA

Use

Drosophila transgenesis

Expression

Bacterial and Insect

Available Since

Feb. 2, 2011

Availability

Academic Institutions and Nonprofits only

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pMOS013: mito-roGFP2-Grx1 glutathione redox sensor (mitochondrial)

Plasmid

#163056

Purpose

mito-roGFP2-Grx1 glutathione redox sensor (mitochondrial)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

mito-roGFP2-Grx1

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pmScarlet3-Giantin_C1

Plasmid

#189773

Purpose

Construct for labelling Golgi apparatus in mammalian cells using mScarlet3-Giantin fusion

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mScarlet3-Giantin

Expression

Mammalian

Promoter

CMV

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pet-15b-microID

Plasmid

#172881

Purpose

expresses microID in E. coli

Depositor

Julien Béthune

Article

Kubitz et al Commun Biol. 2022 Jul 4;5(1):657. doi: 1

Insert

microID

Tags

myc

Expression

Bacterial

Mutation

fragment ([aa2-171]) of aquifex aeolicus BirA wit…

Promoter

T7

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pJH773_pTech_PlldR-HHRz-supP(opal)

Plasmid

#163732

Purpose

Lactate-inducible expression of supP(opal), preceded by a hammerhead ribozyme

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

hhrz-supP

Expression

Bacterial

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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LRP6-pCS2

Plasmid

#27242

Depositor

Xi He

Article

Tamai et al Nature. 2000 Sep 28. 407(6803):530-5.

Insert

low density lipoprotein receptor-related protein 6 (LRP6 Human)

Use

Xenopus injection

Expression

Mammalian

Available Since

Feb. 10, 2011

Availability

Academic Institutions and Nonprofits only

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