164,802 results

1. pAAV-Ef1a-DIO ChETA-EYFP

   Plasmid

   #26968

   Purpose

   AAV expression of humanized ChR2 with E123T/H134R mutations fused to EYFP driven by EF1a promoter for optogenetic activation

   Depositor

   Karl Deisseroth

   Article

   Gunaydin et al Nat Neurosci. 2010 Mar;13(3):387-92. doi

   Has Service

   AAV1, AAV5, and AAV9

   Insert

   hChR2(E123T/H134R)-EYFP
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   E123T, H134R

   Promoter

   EF1alpha

   Available Since

   Jan. 28, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pHIV-IL13-CD8aStalk-PDGFR

   Plasmid

   #240242

   Purpose

   Lentiviral transfer plasmid encoding IL13 with CD8a stalk and PDGFR TM domain

   Depositor

   Michael Birnbaum

   Article

   Dobson et al Nat Methods. 2022 Apr;19(4):449-460. doi

   Insert

   IL-13 (IL13 Human)
   

   Use

   Lentiviral

   Tags

   EGFP and HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   June 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pACYC-GFPM2

   Plasmid

   #102791

   Purpose

   Expresses GFP under WT T7 promoter

   Depositor

   Andrew Ellington

   Article

   Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

   Insert

   Green Fluorescent Protein
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. NSP6-Flag

   Plasmid

   #210345

   Purpose

   Expresses SARS-CoV-2 NSP6 fused with Flag in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   SARS-CoV-2 NSP6 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1, Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Many synonymous changes due to codon optimization

   Promoter

   Available Since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. p3xFLAG CMV14 / BRAF-G469V

   Plasmid

   #131718

   Purpose

   Mammalian Expression of Flag tagged BRAF

   Depositor

   Jacques De Grève

   Article

   Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

   Insert

   BRAF (BRAF Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   G469V

   Promoter

   CMV

   Available Since

   March 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCMVTNT-T7-KPNA7

   Plasmid

   #26683

   Depositor

   Bryce Paschal

   Article

   Kelley et al BMC Cell Biol. 2010 Aug 11;11:63. doi: 1

   Insert

   Karyopherin Alpha 7 (KPNA7 Human)
   

   Use

   Tags

   T7

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. p5xATF6-GL3

   Plasmid

   #11976

   Purpose

   Reporter gene with five ATF6 sites that is highly sensitive to ER stress

   Depositor

   Ron Prywes

   Article

   Wang et al J Biol Chem. 2000 Sep 1. 275(35):27013-2

   Insert

   5xATF6 binding site
   

   Use

   Tags

   Luc and c-fos promoter

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 10, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PRK5-FLAG-Bag6

   Plasmid

   #61836

   Purpose

   express N-terminally FLAG tagged human Bag6

   Depositor

   Yihong Ye

   Article

   Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

   Insert

   Bag6 (BAG6 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. AiP12828 - pAAV-AiE0456m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2828)

   Plasmid

   #224006

   Purpose

   AiE0456m is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical L5_ET neurons

   Depositor

   Jonathan Ting, Allen Institute for Brain Science

   Article

   Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

   Insert

   SYFP2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   NA

   Promoter

   minBG

   Available Since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. AAVS1_Puro_Tet3G_3xFLAG_Twin_Strep

    Plasmid

    #92099

    Purpose

    Doyon lab Tandem-Affinity Purification Following Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus. Transgene expression is controlled by an Auto-Regulated Tet-On 3G System.

    Depositor

    Yannick Doyon

    Article

    Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

    Type

    Empty backbone

    Use

    CRISPR and TALEN; Zfn

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tet-On 3G Bidirectional

    Available Since

    June 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCAG2IG-Hmga1a

    Plasmid

    #122293

    Purpose

    Overexpression for mouse Hmga1a

    Depositor

    Yukiko Gotoh

    Article

    Kishi et al Nat Neurosci. 2012 Aug;15(8):1127-33. do

    Insert

    Hmga1a (Hmga1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTRE-Slow-FT

    Plasmid

    #31915

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al Nat Chem Biol. 2009 Feb;5(2):118-26. Epu

    Insert

    Slow-FT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    M18V/E30V/K69R/L84W/A179V compared to mCherry (bu…

    Promoter

    PTight Tet-responsive PminCMVd

    Available Since

    Dec. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDEST14-ARL3

    Plasmid

    #67376

    Purpose

    Expression of native hArl3 in E. coli

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARL3 (ARL3 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pSmartBAC-S

    Plasmid

    #107268

    Purpose

    Broad host range bacterial artificial chromosome vector

    Depositor

    Ron Godiska, Mark Liles

    Article

    Nasrin et al J Nat Prod. 2018 Jun 22;81(6):1321-1332.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pC0050-CMV-dPspCas13b-longlinker-ADAR2DD(wt)

    Plasmid

    #103866

    Purpose

    dPspCas13b-ADAR2DD(wt) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA. Longer linker than pC0039.

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Inserts

    PspCas13b
    huADAR2DD (ADARB1 Human)
    

    Use

    CRISPR

    Tags

    GSGGGGS and HIV NES

    Expression

    Mammalian

    Mutation

    Changed histidne 133 to alanine and histidine 105…

    Promoter

    Available Since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p663-UBC-miniTurbo-V5-CDK20_IDG-K

    Plasmid

    #189883

    Purpose

    Gateway destination clone of CDK20 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    CDK20 (CDK20 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available Since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCasAb-apr

    Plasmid

    #121998

    Purpose

    Bacterial expression of Cas9 nuclease and RecAb recombination system in Acinetobacter baumannii

    Depositor

    Quanjiang Ji

    Article

    Wang et al Cell Chem Biol. 2019 Sep 17. pii: S2451-

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1-ER-EBFP2

    Plasmid

    #231183

    Purpose

    Expresses control plasmid for ER-mitochondrial linkers, tagged with EBFP2, in mammalian cells

    Depositor

    Dmitry Lim

    Article

    Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

    Insert

    ER-EBFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRDB_182

    Plasmid

    #216103

    Purpose

    CRISPRi, EF1a-driven dCas9-KRAB (ZIM3) (Cas only)

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Insert

    Cas9 [Sp]
    

    Use

    CRISPR and Lentiviral; Assembled vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PB-TA-ERP2

    Plasmid

    #80477

    Purpose

    All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (constitutive rtTA and puromycin resistance)

    Depositor

    Knut Woltjen

    Article

    Kim et al Methods Mol Biol. 2016;1357:111-31. doi:

    Type

    Empty backbone

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge