164,802 results

1. CDK12 gRNA (BRDN0001147156)

   Plasmid

   #75915

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human CDK12

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   CDK12 (CDK12 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBad-EBFP2

   Plasmid

   #14891

   Purpose

   Bacterial expression of EBFP2 blue fluorescent protein

   Depositor

   Robert Campbell

   Article

   Ai et al Biochemistry. 2007 May 22;46(20):5904-10

   Has Service

   Cloning Grade DNA

   Insert

   EBFP2
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   The fluorescent protein was inserted between XhoI…

   Promoter

   Available Since

   May 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLVX-CMV-Galpha-12 ONE-GO

   Plasmid

   #189739

   Purpose

   GPCR/G protein BRET biosensor

   Depositor

   Mikel Garcia-Marcos

   Article

   Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

   Inserts

   Galpha-12 ONE-GO
   GNA12 (GNA12 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 19, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pSpCas9(BB)-2A-mCherry

   Plasmid

   #197420

   Purpose

   Cas9 from S. pyogenes with 2A-mCherry, and cloning backbone for sgRNA

   Depositor

   Torsten Wittmann

   Article

   Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. c-myc promoter (Del 1)

   Plasmid

   #16601

   Depositor

   Bert Vogelstein

   Article

   He et al Science. 1998 Sep 4. 281(5382):1509-12.

   Insert

   c-myc promoter (Del 1) (MYC Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Del-1 (XmnI->PvuII)

   Promoter

   Available Since

   March 24, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. mBFP-APP-mGFP

   Plasmid

   #196694

   Purpose

   Expresses APP695 with two fluorescent proteins at the ends to follow processing in mammalian cells

   Depositor

   Martino Calamai

   Article

   Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:

   Insert

   Amyloid Precursor Protein 695 isoform (APP Human)
   

   Use

   Tags

   mEGFP and mtagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pC1-REX-YFP

   Plasmid

   #48247

   Purpose

   Genetically encoded fluorescent indicator for measuring NAD/NADH ratio.

   Depositor

   Vsevolod Belousov

   Article

   Bilan et al Biochim Biophys Acta. 2013 Nov 25. pii:

   Insert

   REX-YFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 31, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMIH-TFAM-GFP

   Plasmid

   #113704

   Purpose

   Fluorescent fusion protein used to visualise mitochondrial nucleoids, with hygromycin selection

   Depositor

   Benjamin Kile

   Article

   McArthur et al Science. 2018 Feb 23;359(6378). pii: 359

   Insert

   TFAM (Tfam Mouse)
   

   Use

   Retroviral

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   MSCV

   Available Since

   Jan. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pOSIP-KL (KanR, Lambda)

   Plasmid

   #45984

   Depositor

   Drew Endy, Keith Shearwin

   Article

   St-Pierre et al ACS Synth Biol. 2013 Sep 20;2(9):537-41.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBluescriptKS(-)-FRB

    Plasmid

    #104475

    Purpose

    Plasmid carrying FRB with C-terminal HA tag.

    Depositor

    Benjamin Glick

    Article

    Papanikou et al Elife. 2015 Dec 28;4. pii: e13232. doi:

    Insert

    FRB (MTOR Human)
    

    Use

    Phagemid

    Tags

    HA tag

    Expression

    Mutation

    includes only the FRB domain

    Promoter

    Available Since

    Jan. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. CDK12 gRNA (BRDN0001147294)

    Plasmid

    #75916

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CDK12

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    CDK12 (CDK12 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHR-flag-Lag16-iCAM-1

    Plasmid

    #205195

    Purpose

    This vector encodes of the synCAM (iCAM1) and GFP nanobody LAG16

    Depositor

    Wendell Lim

    Article

    Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

    Insert

    synCAM iCAM1 and Lag16 anti-GFP nanobody
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. mEmerald-CLIP170-N-18

    Plasmid

    #54044

    Purpose

    Localization: Microtubule Binding , Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    CLIP170 (CLIP1 Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 2, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. PyronicSF-mRuby2/pBI-CMV1

    Plasmid

    #124830

    Purpose

    Highly resposive GFP-based pyruvate nanosensor.

    Depositor

    Luis Felipe Barros

    Article

    Arce-Molina et al eLife. 2020 Mar 6;9. pii: 53917. doi: 10

    Inserts

    PyronicSF
    mRuby2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. INPP5D-eGFP WT

    Plasmid

    #161998

    Purpose

    Mammalian expression vector, containing a GFP tagged version of INPP5D

    Depositor

    David Bryant

    Article

    Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

    Insert

    INPP5D (INPP5D Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEGFP VARP

    Plasmid

    #42312

    Depositor

    Thierry Galli

    Article

    Burgo et al EMBO Rep. 2009 Oct;10(10):1117-24. doi:

    Insert

    VARP (ANKRD27 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 14, 2013

    Availability

    Academic Institutions and Nonprofits only

17. gCH128 (non-targeting crRNA)

    Plasmid

    #217339

    Purpose

    Non-targeting crRNA

    Depositor

    Luke Gilbert

    Article

    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Insert

    crNT-3
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available Since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pL-CRISPR.SFFV.GFP

    Plasmid

    #57827

    Purpose

    Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. SFFV Promoter driven

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Inserts

    SpCas9
    Sp sgRNA scaffold
    SFFV
    P2A-eGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-CAG-fDIO-oG-WPRE-SV40pA

    Plasmid

    #74291

    Purpose

    pAAV vector to express oG (optimized Glycoprotein) in a Flp-dependent manner

    Depositor

    Edward Callaway

    Article

    Kim et al Cell Rep. 2016 Apr 26;15(4):692-699. doi

    Insert

    oG (optimized Glycoprotein)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    chimeric glycoprotein

    Promoter

    CAG

    Available Since

    April 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAGGs-NLS-TIR1_P2A_NES-TIR1

    Plasmid

    #117699

    Purpose

    Expression of NLS-(Os)TIR1-HA and Myc-NES-(Os)TIR1from one mRNA

    Depositor

    Joerg Mansfeld

    Article

    Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

    Inserts

    TRANSPORT INHIBITOR RESPONSE 1
    TRANSPORT INHIBITOR RESPONSE 1
    

    Use

    Tags

    HA tag, Myc-NES, and NLS

    Expression

    Mammalian

    Mutation

    codon optimized for murine expression

    Promoter

    None, 2A peptide from porcine teschovirus-1 polyp…

    Available Since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCMV-GCaMP5G

    Plasmid

    #31788

    Purpose

    a single-wavelength GCaMP3-based calcium indicator with improved response. Please also see the GCaMP6s/m/f indicators.

    Depositor

    Loren Looger, Douglas Kim, GENIE Project

    Article

    Akerboom et al J Neurosci. 2012 Oct 3;32(40):13819-40.

    Insert

    GCaMP5G
    

    Use

    Tags

    6xHis, T7 epitope, and Xpress tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pDEST47-ARL8B-GFP

    Plasmid

    #67404

    Purpose

    Mammalian expression of hArl8b with C-term GFP tag

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARL8B (ARL8B Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGEX6P1-mClover3-anti-mouse-IgG(kappa)-nanobody

    Plasmid

    #138128

    Purpose

    Expresses GST-tagged mClover3-anti-mouse-IgG(kappa)-nanobody in E. coli cells.

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

    Insert

    mClover3-anti-mouse-IgG(kappa)-nanobody
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. TetOn-CLIMP63-mCherry-eGFP

    Plasmid

    #139871

    Purpose

    Dox-inducible lentiviral expression of human CLIMP63

    Depositor

    Jacob Corn

    Article

    Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

    Insert

    CLIMP63 (CKAP4 Human)
    

    Use

    Lentiviral

    Tags

    mCherry-eGFP

    Expression

    Mutation

    Promoter

    Available Since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. GST-RNF126

    Plasmid

    #138643

    Purpose

    Bacterial expression of GST-RNF126 fusion protein

    Depositor

    Jane McGlade

    Article

    Smith et al J Cell Sci. 2013 Mar 15;126(Pt 6):1366-8

    Insert

    RNF126 (RNF126 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBA707

    Plasmid

    #186238

    Purpose

    KanR-BBR1 entry vector for HR donor

    Depositor

    Jennifer Doudna

    Article

    Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAV.CAG.iGluSnFR3.v857.GPI

    Plasmid

    #178335

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPI

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Insert

    pAAV CAG iGluSnFR3 v857.GPI
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

    Tags

    GPI Anchor, IgK-chain, and Myc epi tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pGWB402

    Plasmid

    #74796

    Purpose

    Gateway cloning compatible binary vector for expression of gene by CaMV35S promoter.

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available Since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCold I-Hero45

    Plasmid

    #187931

    Purpose

    Bacterial expression of heat-resistant obscure (Hero) protein Hero45

    Depositor

    Yukihide Tomari

    Article

    Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

    Insert

    Hero45 (SERBP1 Human)
    

    Use

    Tags

    Flag-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCAGGs-BFP

    Plasmid

    #127348

    Purpose

    Expresses BFP constitutively via the CAGGS promoter in mammalian cells

    Depositor

    Phil Sharp

    Insert

    Blue Fluorescent Protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS Promoter

    Available Since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge