159,557 results

1. mVenus N1

   Plasmid

   #27793

   Depositor

   Steven Vogel

   Article

   Koushik et al Biophys J. 2006 Dec 15. 91(12):L99-L101.

   Has Service

   Cloning Grade DNA

   Insert

   mVenus N1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 19, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTriEx-NTOM20-mVenus-Zdk2

   Plasmid

   #81011

   Purpose

   Expression of Zdk2 anchored to mitochondria

   Depositor

   Klaus Hahn

   Article

   Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

   Insert

   Zdk2
   

   Use

   Tags

   6x His tag, FLAG, NTOM20, and mVenus

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   Available since

   Aug. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMVTNT-T7-KPNA3

   Plasmid

   #26679

   Depositor

   Bryce Paschal

   Article

   Kelley et al BMC Cell Biol. 2010 . 11():63.

   Insert

   Karyopherin Alpha 3 (KPNA3 Human)
   

   Use

   Tags

   T7 (MASMTGGQQMG)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3.1(+)-Lifeact-(GGS)X6-HaloTag C58

   Plasmid

   #171435

   Purpose

   Expression of Lifeact-(GGS)X6-HaloTag C58 in mammalian cells

   Depositor

   Yujie Sun

   Article

   Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

   Insert

   Lifeact-(GGS)X6-HaloTag C58
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR_D17_hrGFP

   Plasmid

   #84616

   Purpose

   HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into D17 locus

   Depositor

   Ian Wheeldon

   Article

   Schwartz et al ACS Synth Biol. 2016 Dec 19.

   Insert

   hrGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   UAS1B8-TEF(136)

   Available since

   Dec. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYFP-ErbB2

   Plasmid

   #66948

   Purpose

   Mammalian expression of rat ErbB2 tagged with YFP

   Depositor

   Bo van_Deurs, Mads Lerdrup

   Article

   Lerdrup et al Mol Biol Cell. 2007 Sep;18(9):3656-66. E

   Insert

   ErbB2 (Erbb2 Rat)
   

   Use

   Tags

   6xHis, V5 Tag, and YFP

   Expression

   Mammalian

   Mutation

   (See depositor comment below on mutations A24T an…

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pSRK2706

   Plasmid

   #78571

   Purpose

   Expresses untagged human rhinovirus 3C protease in E. coli

   Depositor

   David Waugh

   Article

   Raran-Kurussi et al Anal Biochem. 2016 Apr 19;504:30-37. doi

   Insert

   human rhinovirus 3C protease (HRVBgp1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMMP-dnPERK-IRES-eGFP

   Plasmid

   #36954

   Depositor

   Bill Sugden

   Article

   Lee et al Blood. 2008 Feb 15;111(4):2280-9. Epub 2

   Insert

   dnPERK (EIF2AK3 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   deletion of aa592-1061 to create dominant negative

   Promoter

   CMV

   Available since

   July 12, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. mTq2-Calmodulin

   Plasmid

   #198200

   Purpose

   FRET donor: Calmodulin-2 tagged with N-terminal mTurquoise2

   Depositor

   Christian Wahl-Schott

   Article

   Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

   Insert

   Calmodulin 2 (Calm2 Rat)
   

   Use

   Tags

   mTurquoise2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAd/CMV/V5-Clasp2α

    Plasmid

    #89763

    Purpose

    Adenovirus destination vector for transient expression of human wild-type EGFP-CLASP2α

    Depositor

    Torsten Wittmann

    Article

    Pemble et al J Cell Sci. 2017 Apr 15;130(8):1404-1412

    Insert

    CLASP2α (CLASP2 Human)
    

    Use

    Adenoviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. PAM1

    Plasmid

    #198740

    Purpose

    pTFIID-GFP, Geneticin resistance

    Depositor

    Chantal Abergel

    Article

    Philippe et al Nat Protoc. 2023 Nov 14. doi: 10.1038/s4

    Insert

    GFP
    

    Use

    Acanthamoeba expression

    Tags

    Expression

    Mutation

    Promoter

    pTFIID

    Available since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHR SFFVp PHAkt Cerulean

    Plasmid

    #50837

    Purpose

    Fluorescent reporter for membrane PIP3 levels

    Depositor

    Wendell Lim

    Article

    Toettcher et al Nat Methods. 2011 Sep 11;8(10):837-9. do

    Insert

    PHAkt (AKT1 )
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Cerulean

    Expression

    Mutation

    Promoter

    Available since

    Feb. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-Ii-OVA.I+II

    Plasmid

    #64596

    Purpose

    Expression of invariant chain fused to chicken ovalbumin 242-353 amino acids as model antigen for study of MHC class II presentation

    Depositor

    Sandra Diebold, Martin Zenke

    Article

    Diebold et al Gene Ther. 2001 Mar;8(6):487-93.

    Insert

    Ovalbumin
    

    Use

    Tags

    Invariant chain

    Expression

    Mammalian

    Mutation

    Only amino acids 242-353 (class I and class II ep…

    Promoter

    CMV

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. huCaV2.2 (pSAD442-1)

    Plasmid

    #62574

    Purpose

    Human CaV2.2 expression in mammalian cells

    Depositor

    Diane Lipscombe

    Article

    Groen et al Hum Mol Genet. 2015 Feb 15;24(4):987-993

    Insert

    CACNA1B (CACNA1B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pENN.AAV.CB7.CI.mCerulean.WPRE.RBG

    Plasmid

    #105557

    Purpose

    AAV expression of mCerulean from CB7 promoter

    Depositor

    James M. Wilson

    Has Service

    AAV1 and AAV9

    Insert

    mCerulean
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CB7

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-EF1a-mCherry-IRES-Flpo

    Plasmid

    #55634

    Purpose

    Expresses Flpo in Mammalian Cells

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Has Service

    AAV Retrograde and AAV1

    Inserts

    mCherry
    Flpo
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a and Ef1a/IRES

    Available since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTP1172

    Plasmid

    #104161

    Purpose

    Bacterial expression plasmid of anti-mouse kappa chain nanobody TP1170 (3x Cysteines)

    Depositor

    Dirk Görlich

    Article

    Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

    Insert

    Anti-mouse kappa chain specific nanobody TP1170 (3xCysteines)
    

    Use

    Tags

    14xHistidine tag and NEDD8 from Brachypodium dist…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRK5-FLAG-S6K1

    Plasmid

    #100509

    Purpose

    Expresses FLAG tagged S6K1 in mammalian cells

    Depositor

    David Sabatini

    Article

    Gu et al Science. 2017 Nov 10;358(6364):813-818.

    Insert

    S6K1 (Rps6kb1 Rat)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. GPR_control

    Plasmid

    #216222

    Purpose

    GFP P2A RFP control reporter

    Depositor

    Rebecca Voorhees

    Article

    Inglis et al J Cell Sci. 2023 May 15;136(10):jcs26121

    Insert

    GFP P2A RFP_stop
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PLIN2-V5-APEX2

    Plasmid

    #170573

    Purpose

    A C-terminal APEX2 fusion to the mouse perilipin-2 protein PLIN2

    Depositor

    James Olzmann

    Article

    Bersuker et al Dev Cell. 2018 Jan 8;44(1):97-112.e7. do

    Insert

    PLIN2 (Plin2 Mouse)
    

    Use

    Lentiviral

    Tags

    V5-APEX2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pUC-fFuCas9-HTBNLS-hph

    Plasmid

    #121092

    Purpose

    Multigene editing in the Fusarium fujikuroi genome using the CRISPR-Cas9 system

    Depositor

    Xiao-Jun Ji

    Article

    Shi et al ACS Synth Biol. 2019 Feb 15;8(2):445-454

    Insert

    Cas9 and hygromycin resist gene
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    gpdA;trpC

    Available since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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22. pRS306-URA3-PHIS3-AqLumSynth –Sapphire

    Plasmid

    #116931

    Purpose

    Yeast integrative vector for the exression of the 20nm GEM under the HIS3 promoter

    Depositor

    Liam Holt

    Article

    Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

    Insert

    lumazine synthase
    

    Use

    Tags

    Sapphire

    Expression

    Yeast

    Mutation

    Promoter

    HIS3

    Available since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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23. pcDNA3-TLR5-CFP

    Plasmid

    #13019

    Depositor

    Doug Golenbock

    Insert

    Toll-Like Receptor 5 (TLR5 Human)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. K44A dynamin 1 pEGFP

    Plasmid

    #34681

    Depositor

    Sandra Schmid

    Article

    Song et al Mol Biol Cell. 2004 May;15(5):2243-52. E

    Insert

    dynamin 1ba (DNM1L Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    K44A

    Promoter

    Available since

    Jan. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMRX-IZ-ANXA2-mRuby3

    Plasmid

    #184889

    Purpose

    Stable expression of ANXA2-mRuby3 (Annexin A2-mRuby3) in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Yim et al FEBS Lett. 2022 Mar 11. doi: 10.1002/187

    Insert

    ANXA2 (ANXA2 Human)
    

    Use

    Tags

    mRuby3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. p10xQUAS-CsChrimson

    Plasmid

    #163629

    Purpose

    10xQUAS effector expressing CsChrimson.mVenus for red-shifted optogenetic activation of neurons; Contains both P-element ends and attB integration sequence

    Depositor

    Christopher Potter

    Article

    Task et al Elife. 2022 Apr 20;11. pii: 72599. doi:

    Insert

    CsChrimson.mVenus
    

    Use

    Tags

    mVenus

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. FNpCDNA3

    Plasmid

    #45346

    Depositor

    Robert Oshima

    Type

    Empty backbone

    Use

    Tags

    FLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pJMP1

    Plasmid

    #79873

    Purpose

    Bacillus subtilis dCas9 expression vector; integrates into lacA/ganA

    Depositor

    Stanley Qi, Carol Gross, Jason Peters

    Article

    Peters et al Cell. 2016 Jun 2;165(6):1493-506. doi: 1

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    xylA

    Available since

    Oct. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pHEE-R-hyg5A9si

    Plasmid

    #222835

    Purpose

    CRISPR/Cas9 for multi-gene knockout in Arabidopsis (single intron-containing Cas9, RPS5A promoter, hygromycin and red fluorescent seed selection)

    Depositor

    Hidenori Takeuchi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. qTAG-C-mStayGold-Blast

    Plasmid

    #227254

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-2A-Blast cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge