We narrowed to 8,358 results for: BLI

1. p-dCas9-JMJD2A-Hygro

   Plasmid

   #104410

   Purpose

   transient expression of dCas9-JMJD2A fusion protein

   Depositor

   Stephan Beck

   Insert

   JMJD2A (KDM4A Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   April 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. FLT3-MSCV-IRES-GFP

   Plasmid

   #184778

   Purpose

   Expresses wildtype FLT3 in mammalian cells

   Depositor

   Timothy Ley

   Insert

   FLT3 (FLT3 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   MSCV

   Available since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. PRDX6A-c001

   Plasmid

   #175474

   Purpose

   Protein expression in bacterial cells. Entire CDS, M1-P224. Can be biotinylated.

   Depositor

   Opher Gileadi

   Insert

   PRDX6 (PRDX6 Human)
   

   Use

   Tags

   AviTag and His6-TEV

   Expression

   Bacterial

   Mutation

   See Depositor Comments

   Promoter

   Available since

   Feb. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV[Expression]-mCherry:T2A:Hygro-EF1A>Luc2

   Plasmid

   #174665

   Purpose

   Expresses humanized firefly luciferase and mCherry and Hygromycin resistance linked by T2A, each controlled by a strong promoter

   Depositor

   Eli Bar

   Inserts

   LUC2
   mCherry:T2A:Hygro
   

   Use

   Lentiviral and Luciferase

   Tags

   mCherry and hygromycin dual reporter gene linked …

   Expression

   Mammalian

   Mutation

   Promoter

   Human cytomegalovirus immediate early enhancer/pr…

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSyn-Cre-P2A-dTomato

   Plasmid

   #107738

   Purpose

   Can be used to express Cre recombinase and simultaneously the red fluorescent protein dTomato. Can also be used to create adeno-associated virus for delivery of these genes.

   Depositor

   Rylan Larsen

   Has Service

   AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   Cre, dTomato
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBabe-Kras Wt

   Plasmid

   #75282

   Purpose

   Expresses human Kras wild-type

   Depositor

   Channing Der

   Insert

   KRAS (KRAS Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   None. Wild-Type

   Promoter

   Available since

   May 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOET5 CVB3-VLP

   Plasmid

   #218146

   Purpose

   Forms coxsackievirus 3-like particle in insect cells by expression of VP1–VP4 polyprotein (GeneID M33854.1) and 3CD protease (GeneID M33854.1).

   Depositor

   Minna Hankaniemi

   Inserts

   VP1–VP4
   3CD
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   CMV and Polyhedrin

   Available since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. psd44-GqQL

   Plasmid

   #46826

   Purpose

   2nd generation lentiviral vector for expression of mutant G protein alpha q in mammalian cells

   Depositor

   Agnese Mariotti

   Insert

   g protein alpha q (GNAQ Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Q209L (The mutation reduces GTPase activity resul…

   Promoter

   PGK promoter

   Available since

   July 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. AiP14532-pAAV-DLX2.0-minBG-FlpO-WPRE3-BGHpA (Alias: CN4532)

   Plasmid

   #222939

   Purpose

   FlpO recombinase expression from the DLX2.0 enhancer

   Depositor

   Jonathan Ting

   Insert

   FlpO (flp Synthetic)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. 5'UTR CGG 99x FMR1-EGFP

    Plasmid

    #63091

    Purpose

    Contains expanded CGG repeats (99 repeats) within the 5'UTR of FMR1 as found in the Fragile X Tremor Ataxia Syndrome (FXTAS). These repeats are in frame with EGFP.

    Depositor

    Nicolas Charlet-Berguerand

    Insert

    expanded CGG repeats (99 repeats) within the 5'UTR of FMR1 (FMR1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. PB-TO-NFIA.1-SOX9

    Plasmid

    #182310

    Purpose

    Piggybac Tet-ON plasmid for differentiating hiPSCs into astrocytes via NFIA and SOX9 expression

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Inserts

    NFIA-T2A-SOX9 (NFIA, SOX9 Human)
    puroR-T2A-mycNLS-mTagBFP2
    

    Use

    Piggybac

    Tags

    None and T2A-mycNLS-mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a and TRE3G

    Available since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-EF1a-DIO-hM3D(Gq)-mCherry

    Plasmid

    #50460

    Purpose

    Double floxed Gq-coupled hM3D DREADD fused with mCherry under the control of EF1a promoter

    Depositor

    Bryan Roth

    Insert

    hM3D(Gq)-mCherry (CHRM3 Human)
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    See supplemental documents for DREADD mutations

    Promoter

    EF1a

    Available since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. Lamin A (R435C)-mRFP

    Plasmid

    #124274

    Purpose

    Lamin A mutant (R435C), C-terminally tagged with mRFP. Mutation associated with Progeria.

    Depositor

    Eric Schirmer

    Insert

    LMNA (R435C) (LMNA Human)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    LMNA (R435C)

    Promoter

    Available since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. p-dCas9-HDAC1-Hygro

    Plasmid

    #104409

    Purpose

    transient expression of dCas9-HDAC1 fusion protein

    Depositor

    Stephan Beck

    Insert

    HDAC1 (HDAC1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSNAPf-ADRβ2 Control Plasmid

    Plasmid

    #101123

    Purpose

    Expression of SNAP-Tagged β2 Adrenergic Receptor in Mammalian cells

    Depositor

    Ana Egana, New England Biolabs

    Insert

    Beta-2-Adrenergic Receptor (ADRB2 Human)
    

    Use

    Tags

    SNAP-tag (SNAPf)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 27, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pAAV-CMV-FLEX-SaCas9-U6-sgKcnn3

    Plasmid

    #159904

    Purpose

    Mutagenesis of Kcnn3

    Depositor

    Larry Zweifel

    Insert

    Kcnn3 (Kcnn3 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. Tet-pLKO-FOXM1-shRNA-81

    Plasmid

    #89497

    Purpose

    Lentiviral vector with dox inducible expression of shRNA targeting human FOXM1.

    Depositor

    Adam Karpf

    Insert

    FOXM1 (FOXM1 Human)
    

    Use

    Lentiviral and RNAi; Doxycycline inducible

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1/TO

    Available since

    June 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. Tet-pLKO-FOXM1-shRNA-#2

    Plasmid

    #89499

    Purpose

    Lentiviral vector with dox inducible expression of shRNA targeting human FOXM1.

    Depositor

    Adam Karpf

    Insert

    FOXM1 (FOXM1 Human)
    

    Use

    Lentiviral and RNAi; Doxycycline inducible

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1/TO

    Available since

    June 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-CMV-FLEX-SaCas9-U6-sgHtr2a

    Plasmid

    #159908

    Purpose

    Mutagenesis of Htr2a

    Depositor

    Larry Zweifel

    Insert

    Htr2a (Htr2a Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. TLCV2-ApaLI-FLAG-T2A-mTagBFP2-Puro

    Plasmid

    #211462

    Purpose

    Lentivirus backbone expressing a doxycycline-inducible mitochondrial localized ApaLI

    Depositor

    Agnel Sfeir

    Article

    Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

    Insert

    ApaLI
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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