We narrowed to 10,454 results for: ESP

1. pDual-eGFP(Stop66)

   Plasmid

   #63218

   Purpose

   Expression of a non-sense mutant version of eGFP (dark) in bacteria and in mammalian cells. Could be used as a reporter for gene targeting and recombineering in mammalian cells.

   Depositor

   Richard S Myers

   Article

   Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1

   Insert

   His-T7-eGFP(Stop66)
   

   Use

   Lentiviral

   Tags

   T7 and his6

   Expression

   Bacterial and Mammalian

   Mutation

   Changed tyrosine at position 66 with respect to t…

   Promoter

   CMV-EF1α hybrid (CEF)

   Available Since

   April 27, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pCin4-3xFLAG-MKL1 D444-500

   Plasmid

   #19839

   Depositor

   Ron Prywes

   Article

   Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

   Insert

   MKL1 D444-500 (MRTFA Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   deleted amino acids 444-500

   Available Since

   March 16, 2009

   Availability

   Academic Institutions and Nonprofits only

3. pGL3b(1454/3172)P2P-wt

   Plasmid

   #21404

   Depositor

   Eric Metzen

   Article

   Metzen et al Biochem J. 2005 May 1. 387(Pt 3):711-7.

   Insert

   EGLN1 (EGLN1 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   Sept. 9, 2009

   Availability

   Academic Institutions and Nonprofits only

4. pCin4-3xFLAG-MKL1 T450A

   Plasmid

   #19843

   Depositor

   Ron Prywes

   Article

   Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

   Insert

   MKL1 T450A (MRTFA Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   changed Threonine 450 to Alanine

   Available Since

   Dec. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

5. pDual-eGFP(W66)

   Plasmid

   #63217

   Purpose

   Expression of the Celeste (cyan) spectral variant of eGFP in bacteria and in mammalian cells. Could be used as a reporter for quantifying gene targeting and recombineering in mammalian cells.

   Depositor

   Richard S Myers

   Article

   Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1

   Insert

   His-T7-eGFP(W66)
   

   Use

   Lentiviral

   Tags

   His6 and T7

   Expression

   Bacterial and Mammalian

   Mutation

   Changed tyrosine at position 66 with respect to t…

   Promoter

   CMV-EF1α hybrid (CEF)

   Available Since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA5FRT/TO-UBXD5-Strep-HA

   Plasmid

   #113493

   Purpose

   Expression of human UBXD5 with C-terminal strep-HA tag

   Depositor

   Hemmo Meyer

   Article

   Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

   Insert

   UBXD5 (UBXN11 Human)
   

   Tags

   2xstrep-HA

   Expression

   Mammalian

   Mutation

   wildtype without stop codon

   Promoter

   CMV

   Available Since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pWay21-MKP-1L16A/L17A

   Plasmid

   #13478

   Depositor

   Anton Bennett

   Article

   Wu et al Mol Cell Biol. 2005 Jun . 25(11):4792-80

   Insert

   MKP-1L16A/L17A (Dusp1 Mouse)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Changed Leucine 16 to Alanine; changed Leucine 1…

   Available Since

   Dec. 8, 2006

   Availability

   Academic Institutions and Nonprofits only

8. px458_2A_GFP_sgRNA_ELAVL2

   Plasmid

   #106107

   Purpose

   CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting ELAVL2

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

   Insert

   gRNA targeting ELAVL2 (ELAVL2 Human)
   

   Use

   CRISPR

   Available Since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX-2TK cyclin T1 (1-551) (P#422)

   Plasmid

   #14609

   Depositor

   Matija Peterlin

   Article

   Fujinaga et al Proc Natl Acad Sci U S A. 1999 Feb 16. 9

   Insert

   cyclin T1 (1-551) (CCNT1 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   amino acids 1-551

   Available Since

   June 30, 2008

   Availability

   Academic Institutions and Nonprofits only

10. pGY466

    Plasmid

    #166661

    Purpose

    For light-inducible Cre recombinase (LiCre) expression under the Met17 promoter in yeast

    Depositor

    Gaël Yvert

    Article

    Duplus-Bottin et al Elife. 2021 Feb 23;10. pii: 61268. doi:

    Insert

    LiCre
    

    Use

    Cre/Lox

    Expression

    Yeast

    Mutation

    Cre: E340A, D341A

    Promoter

    MET17

    Available Since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCin4-3xFLAG-MKL1 D500-630

    Plasmid

    #19841

    Depositor

    Ron Prywes

    Article

    Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

    Insert

    MKL1 D500-630 (MRTFA Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    deleted amino acids 500-630

    Available Since

    March 16, 2009

    Availability

    Academic Institutions and Nonprofits only

12. p3xFLAG-MKL1 D381-506

    Plasmid

    #19853

    Depositor

    Ron Prywes

    Article

    Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

    Insert

    MKL1 (MRTFA Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    deleted amino acids 381 to 506

    Available Since

    March 16, 2009

    Availability

    Academic Institutions and Nonprofits only

13. pAG69 G6PD 3'UTR mut

    Plasmid

    #12053

    Depositor

    David Bartel

    Article

    Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

    Insert

    G6PD 3'UTR mut (G6PD Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    Mutations in microRNA recognition sites (seed mat…

    Available Since

    June 2, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMIR-31-Reporter

    Plasmid

    #71871

    Purpose

    Mammalian expression vector for the analysis of miR-31 activity

    Depositor

    Mien-Chie Hung

    Article

    Shen et al Nature. 2013 May 16;497(7449):383-7. doi

    Insert

    Reverse complementary sequence of miR-31
    

    Tags

    firefly luciferase

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1 FLAG mTOR 1968-2549 S2035T

    Plasmid

    #23000

    Depositor

    Jing Huang

    Article

    Lomenick et al Proc Natl Acad Sci U S A. 2009 Dec 22;10

    Insert

    mTOR (MTOR Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S2035T aa 1968-2549 only

    Available Since

    May 18, 2010

    Availability

    Academic Institutions and Nonprofits only

16. pGL2-HKII-A

    Plasmid

    #64513

    Purpose

    AS-30D hepatoma hexokinase II 4.3 kbp promoter-luciferase reporter vector

    Depositor

    Peter Pedersen

    Article

    Mathupala et al J Biol Chem. 2001 Nov 16;276(46):43407-1

    Insert

    Type II hexokinase gene, partial cds and promoter region (Hk2 Rat)
    

    Use

    Luciferase

    Tags

    firefly luciferase

    Mutation

    Isolated from AS-30D rat hepatoma

    Promoter

    Hexokinase II

    Available Since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

17. Foxf1 pGEX4T1

    Plasmid

    #122213

    Purpose

    Expression of Foxf1 as a GST fusion protein in bacteria

    Depositor

    Paul Herring

    Article

    Hoggatt et al J Biol Chem. 2013 Oct 4;288(40):28477-87

    Insert

    Foxf1 (Foxf1 Mouse)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGL2-HKII-C

    Plasmid

    #64515

    Purpose

    AS-30D hepatoma hexokinase II 2.1 kbp promoter-luciferase reporter vector

    Depositor

    Peter Pedersen

    Article

    Mathupala et al J Biol Chem. 2001 Nov 16;276(46):43407-1

    Insert

    Type II hexokinase gene, partial cds and promoter region (Hk2 Rat)
    

    Use

    Luciferase

    Tags

    firefly luciferase

    Mutation

    Isolated from AS-30D rat hepatoma

    Promoter

    Hexokinase II

    Available Since

    June 23, 2015

    Availability

    Academic Institutions and Nonprofits only

19. p3xFLAG-CMV-RNF8-N-terminus(1-111)

    Plasmid

    #64674

    Purpose

    Expresses only N terminus of RNF8, amino acids 1 to 111

    Depositor

    Marianne Farnebo

    Article

    Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

    Insert

    Ring finger protein 8 (RNF8 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Only N terminus amino acids 1-111

    Promoter

    CMV

    Available Since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pAG150 FGFR1 3'UTR

    Plasmid

    #12008

    Depositor

    David Bartel

    Article

    Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

    Insert

    N15 3'UTR (FGFR1 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    June 8, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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