We narrowed to 10,134 results for: ada

1. pRSET-XS-VWF

   Plasmid

   #64847

   Purpose

   Expresses aa1594–1670 of VWF A2 domain flanked by Venus and Cerulean and tagged with 7X His at C terminus

   Depositor

   Sriram Neelamegham

   Article

   Dayananda et al Anal Biochem. 2011 Mar 15;410(2):206-13.

   Insert

   Venus_VWF A2 domain aa 1594-1670_Cerulean_TEV_7X His (VWF Human, Synthetic)
   

   Tags

   7X His, Cerulean, and Venus

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. NC15 pGLUE CUL3

   Plasmid

   #36970

   Depositor

   Randall Moon

   Article

   Camp et al J Biol Chem. 2012 Feb 24;287(9):6539-50.

   Insert

   CUL3 (CUL3 Human)
   

   Tags

   CBP tag, HA, and streptag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 10, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pKK-mEGFP-TEV

   Plasmid

   #105777

   Purpose

   Expression of your protein of interest in fusion with green fluorescent protein at the N-terminus (cleavable by TEV). mEGFP is an EGFP A206K mutant with reduced dimerization (PMID: 11988576,22869113).

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   mEGFP-TEV

   Expression

   Mammalian

   Available Since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pT2-CMV/TetO2-EYFP-GW

   Plasmid

   #153309

   Purpose

   Tet-On YFP-tagged Gateway destination vector, to be used together with pT2-TetR-neoR transposon plasmid

   Depositor

   Spyros Petrakis

   Article

   Laidou et al Redox Biol. 2020 Feb 11;32:101458. doi:

   Type

   Empty backbone

   Use

   Transposon

   Tags

   Yellow Fluorescent Protein

   Available Since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDisplay-GRAB_NPYmut

   Plasmid

   #208678

   Purpose

   Expresses the genetically-encoded fluorescent neuropeptide Y (NPY) control sensor GRAB_NPYmut in mammalian cells

   Depositor

   Yulong Li

   Article

   Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

   Insert

   GPCR activation based neuropeptide Y (NPY) control sensor GRAB_NPYmut
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYpet-Optop38i

   Plasmid

   #89749

   Purpose

   Expression of light-regulated p38 inhibitor, Ypet-fused, wild-type photosensor, inhibitor MK3BD3-13F

   Depositor

   Michael Courtney

   Article

   Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

   Insert

   Optop38i3
   

   Use

   Fluorescent protein

   Tags

   Ypet

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJM186A

   Plasmid

   #218127

   Purpose

   Hybrid circuit displaying hsCRBN bait and driving expression of gIII

   Depositor

   David Liu

   Article

   Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

   Inserts

   CRBN (CRBN Human)
   M13 pIII
   

   Tags

   434cI_RR69

   Available Since

   June 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pR26-mBMP7

   Plasmid

   #127374

   Purpose

   Allows for doxycycline-inducible BMP7 expression from the murine ROSA26 safe harbor locus upon CRISPR/Cas9-mediated genomic insertion and stable selection.

   Depositor

   Lance Miller

   Article

   Routh et al Front Immunol. 2020 Feb 13;11:57. doi: 1

   Insert

   BMP7 (Bmp7 Mouse)
   

   Use

   CRISPR and Mouse Targeting

   Available Since

   Feb. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-hSyn-DIO-GRAB_UCN1.0

   Plasmid

   #208668

   Purpose

   Expresses the genetically-encoded fluorescent urocortin (UCN) sensor GRAB_UCN1.0 in neurons

   Depositor

   Yulong Li

   Article

   Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

   Insert

   GPCR activation based urocortin (UCN) sensor GRAB_UCN1.0
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   Oct. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBullet-tgn-c

    Plasmid

    #53072

    Purpose

    destination vector with ECFP-trans golgi network marker (VTI12) for tagged fluorescent protein (C-ter-EYFP) of plant gene

    Depositor

    Joshua Heazlewood

    Article

    Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTIGER_mNeonGreen::3xFLAG::dCrk

    Plasmid

    #131137

    Purpose

    UASp construct for over-expression of Drosophila Crk with N-terminal monomeric NeonGreen and 3X FLAG tag. Compatible with PhiC31-mediated site-specific integration or classical P-element insertion.

    Depositor

    Mark Peifer

    Article

    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

    Insert

    Crk (Crk Fly)
    

    Tags

    3XFLAG and mNeonGreen

    Expression

    Insect

    Available Since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. LLPS1-iLID::EGFP::FTH1 (pBS1042)

    Plasmid

    #185289

    Purpose

    For the mammalian expression of the synthetic protein iLID::eGFP::FTH1 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    iLID::eGFP::FTH1
    

    Expression

    Mammalian

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1_ 2xFLAG-2xSTREP_BACH1_Y11F

    Plasmid

    #159132

    Purpose

    Expresses BACH1 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Lignitto et al Cell. 2019 Jul 11;178(2):316-329.e18. do

    Insert

    BACH1 (BACH1 Human)
    

    Tags

    2X-FLAG _ 2X-STREP

    Expression

    Mammalian

    Mutation

    Y11F

    Promoter

    CMV

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAc5.1B-lambdaN-HA-DmeIF4G-trunc-V5His6_G

    Plasmid

    #146392

    Purpose

    Insect Expression of DmeIF4G-trunc. *Note: this plasmid does not contain an HA-tag as the name implies.

    Depositor

    Elisa Izaurralde

    Article

    Zekri et al Mol Cell Biol. 2009 Dec;29(23):6220-31.

    Insert

    DmeIF4G-trunc (eIF4G1 Fly)
    

    Expression

    Insect

    Mutation

    322-1666 truncated version of elF4G sequence with…

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV_NOPLight-ctr

    Plasmid

    #195579

    Purpose

    Expresses the control sensor NOPLight-ctr in mammalian cells

    Depositor

    Tommaso Patriarchi

    Article

    Zhou et al Nat Commun. 2024 Jun 25;15(1):5353. doi:

    Insert

    NOPLight-ctr
    

    Expression

    Mammalian

    Mutation

    D110A, D130A

    Promoter

    CMV

    Available Since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. p-mCherry-C1-Phobos

    Plasmid

    #98166

    Purpose

    blue-shifted artificial anion conducting channelrhodopsin (aACR). Activation max 466 nm; off-kinetics 10 ms. Codon optimized for mammalian expression.

    Depositor

    Peter Hegemann

    Article

    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

    Insert

    Synthetic construct Phobos gene
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    T59S, E83N, E90Q, E101S, V117R, E123S, T159G, G16…

    Promoter

    CMV (+enhancer)

    Available Since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. p-mCherry-C1-Aurora

    Plasmid

    #98167

    Purpose

    red-shifted artificial anion conducting channelrhodopsin (aACR). Activation up to 600 nm; off-kinetics 260 ms. Codon optimized for mammalian expression.

    Depositor

    Peter Hegemann

    Article

    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

    Insert

    Synthetic construct Aurora gene
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    V59S, E83N, E90Q, E101S, V117R, E123S, P242R, A24…

    Promoter

    CMV (+enhancer)

    Available Since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBullet-end-c

    Plasmid

    #53074

    Purpose

    destination vector with ECFP- endosome marker (RabF2a) for tagged fluorescent protein (C-ter-EYFP) of plant gene

    Depositor

    Joshua Heazlewood

    Article

    Lao et al Plant J. 2014 Jun 6. doi: 10.1111/tpj.12

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV_hSynapsin1_NOPLight1

    Plasmid

    #195580

    Purpose

    Expresses NOPLight1 in neurons

    Depositor

    Tommaso Patriarchi

    Article

    Zhou et al Nat Commun. 2024 Jun 25;15(1):5353. doi:

    Insert

    NOPLight1
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    human Synapsin-1

    Available Since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAVA-Gal11p-LGF2(fs)

    Plasmid

    #127482

    Purpose

    Negative control for the AVA-seq system. Gal11p (lambda CI associated) and LGF2 (with one nucleotide insertion) (RNAp associated) interaction

    Depositor

    Joel Malek

    Article

    Andrews et al J Biol Chem. 2019 Jul 26;294(30):11549-1

    Insert

    Gal11p-LGF2(frame shifted) (GAL11 Budding Yeast)
    

    Expression

    Bacterial

    Mutation

    LGF2 is frame shifted by the insert of one nucleo…

    Available Since

    Nov. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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