We narrowed to 58,550 results for: tra

1. pMX-hTAF10

   Plasmid

   #44340

   Depositor

   Marc Timmers

   Article

   Pijnappel et al Nature. 2013 Mar 28;495(7442):516-9. doi

   Insert

   TAF10 (TAF10 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   LTR

   Available Since

   July 19, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. FLAG-TPD54

   Plasmid

   #172450

   Purpose

   Expression of Tumor Protein D52 like 2 (TPD54/TPD52L2) with N-terminal FLAG tag

   Depositor

   Stephen Royle

   Article

   Larocque et al J Cell Biol. 2020 Jan 6;219(1). pii: jcb

   Insert

   TPD54 (TPD52L2 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSEM415 - mosTI unc-119 Pmex-5 - gfp(nls) - tbb-2

   Plasmid

   #182358

   Purpose

   Positive control for mosTI(unc-119). unc-119 rescue and germline nuclear GFP expression after mosTI insertion.

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Insert

   GFP
   

   Expression

   Worm

   Promoter

   Pmex-5

   Available Since

   May 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPI226

   Plasmid

   #113228

   Purpose

   safe haven knock-in plasmid for the act5 locus, Expression of LifeAct-mCherry

   Depositor

   Rob Kay

   Article

   Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

   Insert

   LifeAct
   

   Use

   Safe haven knock-in plasmid for the act5 locus, l…

   Tags

   mCherry

   Promoter

   act5

   Available Since

   Aug. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. p CIneo-RL-Let7-3xBulgeB-mut

   Plasmid

   #115369

   Purpose

   Expression vector to produce humanized Renilla luciferase with three mutated (seed-sequence mutations) partially complementary binding sites for human let-7 miRNA in the 3'UTR

   Depositor

   Witold Filipowicz, Ramesh Pillai

   Article

   Pillai et al Science. 2005 Sep 2. 309(5740):1573-6.

   Insert

   Renilla Luciferase
   

   Use

   Luciferase

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX-2T-GST-RNMT

   Plasmid

   #112709

   Purpose

   Expresses N-terminally GST-tagged human RNMT in bacterial cells

   Depositor

   Daniel Schoenberg

   Article

   Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10

   Insert

   RNMT (RNMT Human)
   

   Tags

   GST and thrombin cleavage site

   Expression

   Bacterial

   Available Since

   Aug. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV.CAGFLEX.(cyto).iATPSnFR2.S29W.A95K.HaloTag

   Plasmid

   #209655

   Purpose

   Expression of iATPSnFR2, high affinity

   Depositor

   Tim Brown, HHMI-JRC Tool Translation Team

   Article

   Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

   Has Service

   AAV5

   Insert

   iATPSnFR2.S29W.HaloTag
   

   Use

   AAV

   Mutation

   S29W.A95K

   Promoter

   CAG

   Available Since

   Nov. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-pMecp2-dSaCas9-pU6-sgRNA

   Plasmid

   #159174

   Purpose

   Vector B encodes pAAV-pMecp2-dSaCas9-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of dSaCas9-sgRNA in neurons

   Depositor

   Chung Tin

   Article

   Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

   Insert

   dSaCas9
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Promoter

   pMecp2

   Available Since

   Nov. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPBO.ACT005

   Plasmid

   #182715

   Purpose

   Constituitvely expressed CasRx crRNA cloning vector, truncated 3' terminator region

   Depositor

   Joseph Schoeniger

   Article

   Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666

   Insert

   CasRx crRNA cloning backbone
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   Catalytically deactivated R295A, H300A, R849A, H8…

   Promoter

   pJ23119 (TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGT)

   Available Since

   Aug. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-LAP1_2-mCherry

    Plasmid

    #159526

    Purpose

    Expresses mCherry from LAP1_2 promoter

    Depositor

    Esteban Engel

    Article

    Maturana et al Mol Ther Methods Clin Dev. 2020 Apr 14;1

    Insert

    mCherry
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    LAP1_2

    Available Since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDSP15 (5’Δ HygR + loxP + MCS + 5’Δ mScarlet-I)

    Plasmid

    #193853

    Purpose

    Promoterless insertion vector for split HygR/mScarlet-I landing pad

    Depositor

    Patrick Phillips

    Article

    Stevenson et al Elife. 2023 Jul 4;12:RP84831. doi: 10.75

    Insert

    5' rps-0::HygR + 5' mScarlet-I
    

    Use

    CRISPR and Cre/Lox

    Expression

    Worm

    Promoter

    rps-0p

    Available Since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. plko.1/shMCT4 #474

    Plasmid

    #99597

    Purpose

    Knockdown of MCT4 expression

    Depositor

    Eli Bar

    Article

    Lim et al Oncogene. 2014 Aug 28;33(35):4433-41. do

    Insert

    MCT4 (SLC16A3 Human)
    

    Use

    Lentiviral

    Available Since

    Oct. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHAGE N-mNeonGreen (from SARS-CoV-2) IRES puro

    Plasmid

    #170467

    Purpose

    The plasmid codes for the Nucleoprotein (N) of SARS-CoV-2 fused to a mNeonGreen fluorescent protein in its C-terminus. Inserted in a lentiviral backbone. Puromycin resistance

    Depositor

    Raphael Gaudin

    Article

    Bakhache et al bioRxiv 2021

    Insert

    Nucleocapsid
    

    Use

    Lentiviral

    Tags

    mNeonGreen

    Expression

    Mammalian

    Promoter

    EF1

    Available Since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. asPink chromoprotein promoter-less

    Plasmid

    #118005

    Purpose

    For course in "Synthetic Biology: A Lab Manual"

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    RBS, asPink
    

    Use

    Escherichia coli

    Mutation

    BioBrick sites removed

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTSSX-STC

    Plasmid

    #115668

    Purpose

    E.coli expression plasmid of small tetraheme cytochrome (STC) from Shewanella oneidensis MR-1 with cleavable Strep tag

    Depositor

    David Kramer

    Article

    Huang et al J Am Chem Soc. 2020 Jun 10;142(23):10459

    Insert

    small tetraheme cytochrome c
    

    Tags

    Signal peptide (aa1-27)-Strep-II tag- Factor Xa c…

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Oct. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEXL flag Smad3

    Plasmid

    #10920

    Depositor

    Bob Weinberg

    Article

    Liu et al Proc Natl Acad Sci U S A. 1997 Sep 30. 9

    Insert

    Smad3 (SMAD3 Human)
    

    Tags

    flag

    Expression

    Mammalian

    Available Since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

17. pBsVchCAST

    Plasmid

    #203812

    Purpose

    Expression of CRISPR-associated transposases, shuttle vector for Bacillus subtilis / E. coli

    Depositor

    Sheng Yang

    Article

    Yang et al ACS Synth Biol. 2023 Jul 21;12(7):2198-2

    Insert

    VchTniQ, VchCas5/8, VchCas7, VchCas6, VchTnsABC
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMDJ21 - pEXP [Psmu-1 | eFlp recombinase (PATCs) | smu-1 UTR]

    Plasmid

    #159810

    Purpose

    Optimized enzyme for genome editing in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    pEXP [Psmu-1 | eFlp recombinase (PATCs) | smu-1 UTR]
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. KX002: pMVP/PB-DEST

    Plasmid

    #121869

    Purpose

    pMVP destination vector, empty PiggyBac transposon vector backbone

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Piggybac transposon, pmvp gateway destination vec…

    Expression

    Mammalian

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. Str-KDEL_ST-SBP-tagBFP

    Plasmid

    #65266

    Purpose

    synchronize trafficking of ST from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Str-KDEL and truncated sialyltransferase (ST6GAL1 Human)
    

    Tags

    Streptavidin Binding Protein (SBP) and tagBFP

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 43 of ST

    Promoter

    CMV

    Available Since

    June 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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