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pAS_4xhyb PylT sfGFP 150TAG

Plasmid

#154771

Purpose

plasmid with 4xhybrid PylT cassette (Mx1201 G1 PylT hybrid ) and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

Insert

sfGFP

Expression

Mammalian

Mutation

150TAG

Promoter

EF1

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pSUPER.retro.puro-HCF-1-B

Plasmid

#36996

Depositor

Debu Chakravarti

Article

Parker et al Mol Cell Biol. 2012 May;32(9):1654-70. E

Insert

HCF-1 shRNA (HCFC1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 15, 2012

Availability

Academic Institutions and Nonprofits only
pIS1 DICER1 long-mut-all

Plasmid

#21653

Depositor

David Bartel

Article

Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

Insert

Dicer 1 (DICER1 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Mutated let-7 and miR103/107 microRNA binding sit…

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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Ad-HisFlag-USP19CYT

Plasmid

#155245

Purpose

for generating adenoviruses expressing the cytoplasmic isoform of rat USP19

Depositor

Simon Wing

Article

Wiles et al Mol Biol Cell. 2015 Mar 1;26(5):913-23.

Insert

USP19 (Cytoplasmic isoform) (Usp19 Rat)

Use

Adenoviral

Tags

6XHis-3XFlag

Promoter

CMV

Available Since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pIS1 DICER1 long-mut-miR103/107

Plasmid

#21652

Depositor

David Bartel

Article

Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

Insert

Dicer 1 (DICER1 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Mutant miR103/107 microRNA binding sites

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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pCDNA CrkII Y221F

Plasmid

#50732

Purpose

mammalian expression of CrkII Y221F mutant

Depositor

Kenneth Yamada

Article

Takino et al J Cell Sci. 2003 Aug 1;116(Pt 15):3145-5

Insert

CrkII Y221F (CRK Human)

Expression

Mammalian

Mutation

Y221F

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pPN022

Plasmid

#91668

Purpose

Express sgRNA targeting human SHANK3

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

SHANK3 (SHANK3 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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KA1874_pGL4-UBCmin-luc2/Sox2_SRR1

Plasmid

#124197

Purpose

Luciferase reporter driven by the Sox2 enhancer

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

Sox2 enhancer

Use

Luciferase

Promoter

UBCmin

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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KA2627_pGL4-UBCmin-luc2/Jam2-A'

Plasmid

#124200

Purpose

Luciferase reporter driven by the Jam2 enhancer

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

Jam2 enhancer

Use

Luciferase

Promoter

UBCmin

Available Since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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KA1867_pGL4-UBCmin-luc2/Pou5f1_CR2

Plasmid

#124193

Purpose

Luciferase reporter driven by the Pou5f1 enhancer

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

Pou5f1 enhancer

Use

Luciferase

Promoter

UBCmin

Available Since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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KA1866_pGL4-UBCmin-luc2/Pou5f1_CR3

Plasmid

#124192

Purpose

Luciferase reporter driven by the Pou5f1 enhancer

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

Pou5f1 enhancer

Use

Luciferase

Promoter

UBCmin

Available Since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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KA1865_pGL4-UBCmin-luc2/Pou5f1_CR4

Plasmid

#124191

Purpose

Luciferase reporter driven by the Pou5f1 enhancer

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

Pou5f1 enhancer

Use

Luciferase

Promoter

UBCmin

Available Since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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KA1403_pGL4-UBCmin-luc2/Nanog-B'

Plasmid

#124190

Purpose

Luciferase reporter driven by the Nanog enhancer

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

Nanog enhancer

Use

Luciferase

Promoter

UBCmin

Available Since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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KA1402_pGL4-UBCmin-luc2/Nanog-A'

Plasmid

#124189

Purpose

Luciferase reporter driven by the Nanog enhancer

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

Nanog enhancer

Use

Luciferase

Promoter

UBCmin

Available Since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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pKC123

Plasmid

#134240

Purpose

expresses gRNA targeting CENP A 3'

Depositor

Iain Cheeseman

Article

Swartz et al Dev Cell. 2019 Oct 7;51(1):35-48.e7. doi

Insert

CENP A 3' CRISPR construct

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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p5xFbx15 (-544/-527)-TK-Luc/Sox-m1

Plasmid

#15687

Depositor

Shinya Yamanaka

Article

Tokuzawa et al Mol Cell Biol. 2003 Apr . 23(8):2699-708

Insert

5 x mutated Fbx15 enhancer (Oct/Sox binding site)

Use

Luciferase

Mutation

Mutated in Sox binding site

Available Since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only
pAAV-EF1a-SwitchON_mRubyNLS-hChR2(H134R)-EYFP-NO_WPRE

Plasmid

#118279

Purpose

Expresses mRuby2 in the nucleus of Cre-negative cells, and ChR(H134R)-YFP in Cre-positive cells

Depositor

Dietmar Schmitz

Article

Wozny et al Front Cell Neurosci. 2018 Oct 2;12:337.

Inserts

mRuby2
ChR2(H134R)

Use

AAV and Cre/Lox

Tags

SV40 NLS and YFP

Promoter

EF1 alpha

Available Since

Dec. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pSR06

Plasmid

#69152

Purpose

read-outloxN mCherry to GFP switch for integration on ttTi5605, Mos Chr II

Depositor

Sander van den Heuvel

Article

Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

Inserts

mCherry
eGFP

Use

Cre/Lox; Mossci

Expression

Worm

Mutation

codon-optimzed index 1.0 and codon-optimzed index…

Promoter

rps-27

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pPHR-EYFP

Plasmid

#103817

Purpose

Soluble BLInCR mock effector that is recruited to 'localizer' sites upon blue light illumination

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

PHR-EYFP (CRY2 Mustard Weed)

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pMSCV FHA-Stop

Plasmid

#27050

Depositor

Jayanta Debnath

Article

Radoshevich et al Cell. 2010 Aug 20. 142(4):590-600.

Insert

autophagy-related 12 (yeast) (Atg12 Mouse)

Use

Retroviral

Tags

HA and flag

Expression

Mammalian

Mutation

changed glycine 141 to stop codon.

Available Since

Jan. 13, 2011

Availability

Academic Institutions and Nonprofits only