59,541 results

1. pBJG1 T7-8xHis-OGT

   Plasmid

   #154271

   Purpose

   Bacterial expression of full length human OGT. N-terminal T7_leader-8xHis tag followed by HRV3C protease site.

   Depositor

   Suzanne Walker

   Article

   Gross et al J Am Chem Soc. 2005 Oct 26;127(42):14588

   Insert

   O-GlcNAc Transferase (OGT Human)
   

   Tags

   8x His, HRV3C protease site, and T7 leader

   Expression

   Bacterial

   Mutation

   bacterial codon optimized

   Available Since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.2 TDP-43 5F-L YFP

   Plasmid

   #84914

   Purpose

   Mammalian expresion of TDP-43 5F-L YFP

   Depositor

   Aaron Gitler

   Article

   Elden et al Nature. 2010 Aug 26;466(7310):1069-75. d

   Insert

   TDP-43 (TARDBP Human)
   

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   F147L, F149L, F194L, F229L, F231L

   Promoter

   CMV

   Available Since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR223-EGFR

   Plasmid

   #23935

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   EGFR (EGFR Human)
   

   Use

   Gateway donor vector

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR221-SPNS1_STOP

   Plasmid

   #161439

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SPNS1 (SPNS1 Human)
   

   Expression

   Mammalian

   Available Since

   Sept. 15, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pMKO.1 puro p53 shRNA 1

   Plasmid

   #10671

   Depositor

   William Hahn

   Article

   Masutomi et al Cell. 2003 Jul 25. 114(2):241-53.

   Insert

   p53 shRNA (TP53 Human)
   

   Use

   RNAi and Retroviral

   Expression

   Mammalian

   Available Since

   Nov. 2, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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6. pmCherry-TPX2

   Plasmid

   #31227

   Depositor

   Patricia Wadsworth

   Article

   Ma et al Mol Biol Cell. 2010 Mar 15. 21(6):979-88

   Insert

   TPX2 (TPX2 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   July 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. iLID-mCl3-MAP7N

   Plasmid

   #190167

   Purpose

   Expresses iLID-mCl3-MAP7N, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60, in mammalian cells.

   Depositor

   Anna Akhmanova

   Article

   Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

   Insert

   iLID-mClover3-MAP7N
   

   Tags

   iLID-mClover3

   Expression

   Mammalian

   Mutation

   aa 1-309 from human MAP7 delta175-213

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Human DsRed-Monomer CASP9 C287A Mutant

   Plasmid

   #198469

   Purpose

   Expresses Human DsRed-Monomer CASP9 C287A Mutant Fusion Protein in Mammalian Cells

   Depositor

   Hannah Rabinowich

   Insert

   Human Caspase-9 C287A Mutant (CASP9 Human)
   

   Tags

   DsRed-monomer

   Expression

   Mammalian

   Mutation

   Changed Cysteine 287 to Alanine to eliminate prot…

   Promoter

   CMV

   Available Since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. N-terminal 3X Flag Human CASP9 C287A Mutant

   Plasmid

   #198380

   Purpose

   Expresses Human N-Terminal 3X Flag CASP9 Catalytic Mutant in Mammalian Cells

   Depositor

   Hannah Rabinowich

   Article

   Han et al Autophagy. 2021 Jun;17(6):1393-1409. doi

   Insert

   N-Terminal 3X Flag Human Caspase-9 C287A Mutant (CASP9 Human)
   

   Tags

   3X Flag

   Expression

   Mammalian

   Mutation

   Changed Cysteine 287 to Alanine to eliminate prot…

   Promoter

   CMV

   Available Since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. C1-MPAct-mCherry

    Plasmid

    #155222

    Purpose

    Monitor changes in the density of membrane-proximal F-actin (MPA)

    Depositor

    Tobias Meyer

    Article

    Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

    Insert

    F-tractin (aa 9-52 of rat ITPKA) fused to mCherry C-term tagged with the CaaX sequence derived from Kras4b
    

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pWZL Neo Myr Flag BTK

    Plasmid

    #20432

    Depositor

    William Hahn, Jean Zhao

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    BTK (BTK Human)
    

    Use

    Retroviral

    Tags

    Flag and Myr

    Expression

    Mammalian

    Available Since

    Oct. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

12. pmTurquoise2-Peroxi

    Plasmid

    #36203

    Purpose

    In vivo visualization of the peroxisome (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

    Insert

    Peroxisomal targeting sequence
    

    Tags

    mTurquoise2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. HTATIP (2OU2)

    Plasmid

    #33338

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    HTATIP (KAT5 Human)
    

    Tags

    Hexahistidine tag with Thrombin cleavage

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 11, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

14. GFP-JIP4

    Plasmid

    #164620

    Purpose

    Expresses JIP4 in mammalian cells with a GFP tag

    Depositor

    Mark Cookson

    Article

    Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

    Insert

    JIP4 (SPAG9 Human)
    

    Tags

    cycle 3 GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. qTAG-C-Solo-mStayGold-CANX

    Plasmid

    #227280

    Purpose

    Donor template for mStayGold insertion into the C-terminus of the CANX locus. For ER visualization. To be co-transfected with sgRNA plasmid px330-CANX (Addgene #227279)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    CANX Homology Arms flanking a mStayGold Tag (CANX Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Promoter

    Promoterless

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. STARR-seq luciferase validation vector_ORI_neg.cont

    Plasmid

    #99315

    Purpose

    Negative control for luciferase validation vectors

    Depositor

    Alexander Stark

    Article

    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

    Insert

    neutral region; chr3: 55198740-55200041
    

    Use

    Luciferase; Starr-seq luciferase validation vector

    Expression

    Mammalian

    Available Since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCL1177

    Plasmid

    #217961

    Purpose

    Dual cistronic expression vector for the RAPID-release approach. H3.2-mCherry is released from mitochondria to the nucleus upon addition of rapamycin.

    Depositor

    Andrew Bowman

    Article

    Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755

    Insert

    Histone H3 (HIST2H3PS2 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1(+)-AKAP95-FRB

    Plasmid

    #138254

    Purpose

    Full-length AKAP95 fused to FRB (rapamycin-binding domain). Used in conjunction with pcDNA3-FKBP-ICUE3-NLS to monitor cAMP dynamics within AKAP95 nanodomain.

    Depositor

    Jin Zhang

    Article

    Clister et al Cell Chem Biol. 2019 Jun 20;26(6):885-89

    Insert

    AKAP95-FRB (AKAP8 Human)
    

    Tags

    FRB rapamycin binding domain and HA epitope

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. SuExp-hPLD4

    Plasmid

    #201244

    Purpose

    express full length human PLD4 protein

    Depositor

    David Nemazee

    Article

    Yuan et al Structure. 2024 Jun 6;32(6):766-779.e7.

    Insert

    PLD4 (PLD4 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. TFORF0701

    Plasmid

    #143065

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    PAXBP1 (PAXBP1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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