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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 6,351 results for: Rel

Showing: 6241 - 6260 of 6351 results

  1. CRHR1-DuET

    Plasmid
    #213215
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CRHR1 (CRHR1 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213215-plasmid-map-sequence-id-430777

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  3. pET32bplus_trxAChEwt

    Plasmid
    #83916
    Purpose
    Encodes an E. coli codon optimized version of wild type human acetylcholinesterase
    Depositor
    Sarel Fleishman
    Article
    Goldenzweig et al Mol Cell. 2016 Jul 21;63(2):337-46. doi:
    Insert
    Short form of hAChE, codon optimised for E.coli expression (ACHE Synthetic, Human)
    Use
    Tags
    His, S-tag, and TRX
    Expression
    Bacterial
    Mutation
    codon optimised for E.coli expression
    Promoter
    T7 promoter
    Available since
    March 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    83916-plasmid-map-sequence-id-174926

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  4. kiCAP-AAV-MyoAAV3D

    Plasmid
    #224445
    Purpose
    Rep/Cap plasmid for the production of MyoAAV 3D, a muscle-tropic AAV capsid in mice and macaques.
    Depositor
    Pardis Sabeti
    Article
    Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.
    Insert
    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    Use
    AAV
    Tags
    Expression
    Mutation
    RGDYREL insert between amino acids 588 and 589 of…
    Promoter
    p41
    Available since
    Sept. 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    224445-plasmid-map-sequence-id-446645

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  5. pcDNA3-N-HA-NEK7 K64M

    Plasmid
    #75143
    Purpose
    Expresses N-terminal HA-tagged NEK7 (with a K64M substitution; kinase dead) in mammalian cells
    Depositor
    Bruce Beutler
    Article
    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:
    Insert
    NIMA (never in mitosis gene a)-related expressed kinase 7 (Nek7 Mouse)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Lys 64 mutated to Met; kinase inactive and F236L …
    Promoter
    CMV
    Available since
    May 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    75143-plasmid-map-sequence-id-147264

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  7. pCRI005-pYFAC-riboB-PgpdA-dLbCpf1(D156R)-VPR-TtrpC

    Plasmid
    #140198
    Purpose
    Episomal expression of dLbCpf1(D156R)-VPR, variant for culture at room temperature. Filamentous fungi vector with AMA1 and riboB selection marker.
    Depositor
    Yit Heng Chooi
    Article
    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102
    Insert
    dLbCas12a(D156R)-VPR
    Use
    CRISPR and Synthetic Biology; Expression in asper…
    Tags
    VPR (VP64-p65-Rta), NLS
    Expression
    Mutation
    D832A DNase deactivated, D156R for improved activ…
    Promoter
    PgpdA
    Available since
    Sept. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    140198-plasmid-map-sequence-id-309713

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  10. pAAV-CMV-CASANOVA

    Plasmid
    #113036
    Purpose
    AAV vector for CASANOVA (AcrIIA4-LOV2 hybrid) expression in mammalian cells; enables optogenetic control of SpyCas9
    Depositor
    Dominik Niopek
    Article
    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s
    Insert
    CASANOVA (for CRISPR/Cas9 activity switching via a novel optogenetic variant of AcrIIA4)
    Use
    AAV, CRISPR, and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    113036-plasmid-map-sequence-id-226953

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  12. pW210-pPB-GA-inducible-CRISPRa-sa_dCas9-VPR-hygR

    Plasmid
    #170808
    Purpose
    PiggyBac vector to express GA-inducible VPR-Sa_dCas9 with hygromycin resistance
    Depositor
    Kenneth Yamada
    Article
    Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.
    Inserts
    GID1-VPR-IRES-HygR
    GAI-tagBFP-Sa_dCas9 (GAI Synthetic, Mustard Weed, S. aureus)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Synonymous mutation in PYL1 to remove XhoI site (…
    Promoter
    Available since
    June 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    170808-plasmid-map-sequence-id-334406

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Showing: 6241 - 6260 of 6351 results