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Showing: 6241 - 6260 of 56458 results

  14. U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_LMG18311_2xUGI_3xHA

    Plasmid
    #136657
    Purpose
    Expresses St1BE4max-LMG18311 in mammalian cells along with its U6-driven sgRNA
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:
    Inserts
    St1Cas9 LMD9:LMG18311 chimera
    sgRNA for St1Cas9
    APOBEC-1 (APOBEC1 Human)
    UGI
    HA tag
    Use
    CRISPR and Synthetic Biology
    Tags
    2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and U6
    Available since
    Feb. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    136657-plasmid-map-sequence-id-273344

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  15. U6_sgRNA_CAG_APOBEC-1_hSt1Cas9_CNRZ1066_2xUGI_3xHA

    Plasmid
    #136654
    Purpose
    Expresses St1BE4max-CNRZ1066 in mammalian cells along with its U6-driven sgRNA
    Depositor
    Yannick Doyon
    Article
    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:
    Inserts
    St1Cas9 LMD9:CNRZ1066 chimera
    sgRNA for St1Cas9
    APOBEC-1 (APOBEC1 Human)
    UGI
    HA tag
    Use
    CRISPR and Synthetic Biology
    Tags
    2xUGI, APOBEC-1, SV40 NLS, UGI, and hSt1Cas9
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and U6
    Available since
    Feb. 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    136654-plasmid-map-sequence-id-273340

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  17. MBP-rPKL S113TAG

    Plasmid
    #107157
    Purpose
    MBP-rPKL S113TAG for production and purification of rat PKL pS113
    Depositor
    Jesse Rinehart
    Article
    Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e
    Insert
    MBP-tagged rat pyruvate kinase liver isoform (Pklr Rat)
    Use
    Tags
    C-term 6-his and N-term MBP
    Expression
    Bacterial
    Mutation
    Serine 113 changed to TAG for phosphoserine inser…
    Promoter
    PBAD
    Available since
    Dec. 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    107157-plasmid-map-sequence-id-204885

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  19. pABD823

    Plasmid
    #67911
    Purpose
    Protein interaction with yeast two hybrid assay of CTG10 in yeast, Saccharomyces cerevisiae
    Depositor
    Bruce Downie
    Article
    Majee et al Proc Natl Acad Sci U S A. 2018 Apr 24;11
    Insert
    Cold temperature germinating 10 (CTG10)
    Use
    Tags
    GAL4 DNA binding domain (148 amino acids)
    Expression
    Yeast
    Mutation
    Promoter
    pADH1 (alcohol dehydrogenase 1 promoter)
    Available since
    Dec. 17, 2018
    Availability
    Academic Institutions and Nonprofits only

  20. pABD933

    Plasmid
    #67917
    Purpose
    Entry clone made prior to studies of protein co-localization of CTG10 via fluorescent fusion after transient expression in planta
    Depositor
    Bruce Downie
    Article
    Majee et al Proc Natl Acad Sci U S A. 2018 Apr 24;11
    Insert
    CTG10
    Use
    Gateway cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 6241 - 6260 of 56458 results