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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 6,520 results for: sas;

Showing: 6241 - 6260 of 6520 results

  2. pAF123

    Plasmid
    #135921
    Purpose
    All-in-one Sleeping Beauty vector for stable transfection of eukaryotic cells with hygromycin restistance. CMV protomor drives SB100X transposase for transposon integration.
    Depositor
    Andrew S. Flies
    Article
    Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab
    Type
    Empty backbone
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Jan. 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    135921-plasmid-map-sequence-id-270083

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  5. pTXB1-TP3

    Plasmid
    #160086
    Purpose
    Bacterial expression of Tn5-APEX2 fusion protein with linker AEAAAKEAAAKA
    Depositor
    Pier Pandolfi
    Article
    Lee et al Nat Methods. 2021 Mar;18(3):293-302. doi
    Insert
    Tn5 transposase/APEX2 peroxidase fusion protein
    Use
    Tags
    FLAG and Mxe intein - Chitin-binding domain
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    160086-plasmid-map-sequence-id-313733

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  6. pTXB1-TP5

    Plasmid
    #160088
    Purpose
    Bacterial expression of Tn5-APEX2 fusion protein with linker GSGAGA
    Depositor
    Pier Pandolfi
    Article
    Lee et al Nat Methods. 2021 Mar;18(3):293-302. doi
    Insert
    Tn5 transposase/APEX2 peroxidase fusion protein
    Use
    Tags
    FLAG and Mxe intein - Chitin-binding domain
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    160088-plasmid-map-sequence-id-313736

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  7. PB-3xCTCF-TetO

    Plasmid
    #213023
    Purpose
    piggyBac donor vector containing an 8-kb TetO operator array flanked by 3xCTCF sites for random integration using PBase transposase
    Depositor
    Luca Giorgetti
    Article
    Mach et al Nat Genet. 2022 Dec;54(12):1907-1918. do
    Insert
    3xCTCF-TetO
    Use
    Mouse Targeting; Piggybase integration (transposo…
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    April 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    213023-plasmid-map-sequence-id-428697

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  8. pSL0375 (His-MBP-TniQ_Cas8_Cas7_Cas6)

    Plasmid
    #130640
    Purpose
    Expresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from a T7 promoter, with N-terminal His10-MBP-TEVsite tag on TniQ for purification.
    Depositor
    Samuel H. Sternberg
    Article
    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:
    Insert
    VchTniQ, VchCas8, VchCas7, VchCas6
    Use
    CRISPR; Transposon
    Tags
    His10-MBP-TEVsite on TniQ
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    Sept. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    130640-plasmid-map-sequence-id-255648

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  9. pTS2362_Tier3(SB)-rtTA-PuroR

    Plasmid
    #169658
    Purpose
    Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-PuroR-pA-3'ITR)
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PPGK-driven rtTA expression and PRPBSA-driven PuroR expression
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PRPBSA
    Available Since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    169658-plasmid-map-sequence-id-365631

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  16. AAVS1_puro_CAG_HexaPro

    Plasmid
    #164077
    Purpose
    Targeted integration of SARS-CoV-2 spike HexaPro variant into the AAVS1 genomic safe harbor locus
    Depositor
    Yannick Doyon
    Article
    Rivest et al protocols.io 2021
    Insert
    SARS-CoV-2 S HexaPro (S Severe acute respiratory syndrome coronavirus 2)
    Use
    CRISPR
    Tags
    2X Strep-Tag II, 8X His tag, and HRV 3C cleavage …
    Expression
    Mammalian
    Mutation
    Ectodomain only (AAs 1-1208); 682-685 (furin site…
    Promoter
    CAG
    Available Since
    Jan. 4, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    164077-plasmid-map-sequence-id-320680

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  17. pSL0374 (His-MBP-Cas8_Cas7_Cas6)

    Plasmid
    #130639
    Purpose
    Expresses V. cholerae CAST Cas8, Cas7, and Cas6 from a T7 promoter, with N-terminal His10-MBP-TEVsite tag on Cas8 for purification.
    Depositor
    Samuel H. Sternberg
    Article
    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:
    Insert
    VchCas8, VchCas7, VchCas6
    Use
    CRISPR; Transposon
    Tags
    His10-MBP-TEVsite on Cas8
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    Sept. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    130639-plasmid-map-sequence-id-255644

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  18. pTXB1-TP4

    Plasmid
    #160087
    Purpose
    Bacterial expression of Tn5-APEX2 fusion protein with linker (GGGGS)4
    Depositor
    Pier Pandolfi
    Article
    Lee et al Nat Methods. 2021 Mar;18(3):293-302. doi
    Insert
    Tn5 transposase/APEX2 peroxidase fusion protein
    Use
    Tags
    FLAG and Mxe intein - Chitin-binding domain
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    160087-plasmid-map-sequence-id-313735

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  20. pOPO685

    Plasmid
    #195300
    Purpose
    pBAD322-MycaIDCas-PaqCI, arapBAD promoter expressing type I-D Cascade and one spacer array of McCAST (Tn7575), the single spacer is replaced with PaqCI entry site.
    Depositor
    Joseph Peters
    Article
    Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765
    Insert
    McCAST Cascade and native array with PaqCI entry site
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Arabinose inducible arapBAD
    Available Since
    May 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    195300-plasmid-map-sequence-id-394739

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Showing: 6241 - 6260 of 6520 results