164,802 results

1. pENTR223-LMP1

   Plasmid

   #195805

   Purpose

   Gateway Entry Clone of Epstein-Barr virus LMP1

   Depositor

   Eric Johannsen

   Article

   Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

   Insert

   LMP1 (LMP-1 Epstein-Barr virus)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1_ 2xFLAG-2xSTREP_NRF2

   Plasmid

   #159129

   Purpose

   expresses NRF2 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Lignitto et al Cell. 2019 Jul 11;178(2):316-329.e18. do

   Insert

   NRF2 (NFE2L2 Human)
   

   Use

   Tags

   2X-FLAG _ 2X-STREP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAGGS_VP35_EBOV

   Plasmid

   #103050

   Purpose

   Encodes Ebola virus VP35 gene (Zaire ebolavirus, Mayinga) under control of the CAG promoter

   Depositor

   Elke Mühlberger

   Article

   Nelson et al Antiviral Res. 2017 Aug 12;146:21-27. do

   Insert

   VP35 gene of Ebola virus (VP35 (Zaire ebolavirus, Mayinga))
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Dec. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBAD-mTagBFP2

   Plasmid

   #34632

   Depositor

   Vladislav Verkhusha

   Article

   Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

   Insert

   mTagBFP2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   I174A compared to mTagBFP (enhanced photostabilit…

   Promoter

   Available Since

   Jan. 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO-perox-tagRFP

   Plasmid

   #185600

   Purpose

   Lentiviral construct for expression of tagRFP-T targeted to peroxisomes

   Depositor

   Jarrod Johnson

   Article

   Greiner et al Curr Protoc. 2022 Aug;2(8):e514. doi: 10

   Insert

   perox-tagRFP-T
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pACYC-Skp-GroEL/ES

   Plasmid

   #83922

   Purpose

   Co-expresses cytoplasmic copies of the Skp and Gro EL/ES protein folding chaperones.

   Depositor

   Karl Griswold

   Article

   Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

   Inserts

   Skp
   Gro EL/ES
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. lenti sgRNA(MS2)_puro optimized backbone

   Plasmid

   #73797

   Purpose

   optimized lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

   Depositor

   Feng Zhang

   Article

   Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and EF1A

   Available Since

   March 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-MP-84-spCas9-U6-gRNA

   Plasmid

   #206935

   Purpose

   Expression of spCas9 endonuclease and gRNA in an AAV vector allowing packaging of viral genome smaller than 5 kb.

   Depositor

   Markus Grompe

   Insert

   spCas9
   

   Use

   AAV

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   MP-84

   Available Since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3 IKKE K38A flag

   Plasmid

   #26202

   Depositor

   Tom Maniatis

   Article

   Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

   Insert

   I-kappa-B kinase epsilon K38A (IKBKE Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Catalytic Lysine changed to Alanine (K38A)

   Promoter

   Available Since

   Aug. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. hSTARR-seq_SCP1 vector_blocking 4

    Plasmid

    #99319

    Purpose

    Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.

    Depositor

    Alexander Stark

    Article

    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

    Type

    Empty backbone

    Use

    Hstarr-seq screening vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. JME4580

    Plasmid

    #129659

    Purpose

    HPHex_CrisprCas9-yl_RFP: CAS9 vector with Hygromycinex marker for gRNA cloning using GoldenGate

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Biotechnol Lett. 2020 Jan 23. pii: 10.10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAG426GPD-ccdB

    Plasmid

    #14156

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 16, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGL3 Control Vector

    Plasmid

    #212937

    Purpose

    Control vector with constitutive firefly luciferase expression

    Depositor

    Debrya Groskreutz

    Insert

    luc+
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    WT

    Promoter

    SV40

    Available Since

    Jan. 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. myrAkt delta4-129

    Plasmid

    #10841

    Depositor

    Richard Roth

    Article

    Kohn et al J Biol Chem. 1996 Sep 6. 271(36):21920-6

    Insert

    Akt (AKT1 Human)
    

    Use

    Tags

    HA and myr

    Expression

    Mammalian

    Mutation

    Deletion of PH domain (aa4-129)

    Promoter

    SV40

    Available Since

    Feb. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. Lenti-sgNT.2#2/Cre

    Plasmid

    #173661

    Purpose

    Expresses a NT.2-targeting gRNA and Cre-recombinase

    Depositor

    Charles Swanton

    Article

    Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

    Insert

    Non-targeting gRNA
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. mCherry- SEC61B

    Plasmid

    #121160

    Purpose

    Mammalian expression of mCherry tagged SEC61B, used as a general ER marker

    Depositor

    Christine Mayr

    Article

    Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

    Insert

    SEC61B (SEC61B )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-hsyn-DIO-Rpl22l1-3Flag-2A-eGFP-WPRE

    Plasmid

    #214265

    Purpose

    Expression of a Flag-tagged Rpl22I1 protein in cells expressing Cre recombinase allowing for the harvesting of actively transcribed mRNAs in that cell type.

    Depositor

    D. James Surmeier

    Insert

    Flag-tagged Rpl22I1 protein expressing Cre Recombinase (Rpl22 Mouse)
    

    Use

    AAV

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    Synapsin

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDisplay-GACh2.0

    Plasmid

    #106073

    Purpose

    express the genetically-encoded fluorescent ACh indicator GACh2.0 in mammalian cells

    Depositor

    Yulong Li

    Article

    Jing et al Nat Biotechnol. 2018 Sep;36(8):726-737.

    Insert

    GPCR activation based ACh sensor (GACh) 2.0
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-PS11-scFvFc-CD28-gp41 (706-713)

    Plasmid

    #60606

    Purpose

    Mammalian Display vector

    Depositor

    Wayne Marasco

    Article

    Taube et al PLoS One. 2008 Sep 11;3(9):e3181. doi: 1

    Insert

    PS11-scFvFc-CD28-gp41 (706-713) (CD28 Human)
    

    Use

    Tags

    C9

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

20. ND6-DdCBE-left side TALE

    Plasmid

    #157842

    Purpose

    expresses left-side TALE–DddAtox half of ND6-DdCBE in mammalian cells

    Depositor

    David Liu

    Article

    Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

    Insert

    SOD2 MTS–3x-HA–ND6 left tale–G1397 DddA-N–UGI–SOD2 3'UTR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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