159,557 results

1. pLenti-C-STAT1-mGFP-P2A-Puro

   Plasmid

   #199345

   Purpose

   express murine STAT1 protein fused with mGFP

   Depositor

   Massimo Mallardo

   Article

   Caiazza et al Int J Mol Sci. 2022 Nov 17;23(22):14232.

   Insert

   signal transducer and activator of transcription 1 (Stat1 Mouse)
   

   Use

   Lentiviral

   Tags

   mGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. Epac-S-H81

   Plasmid

   #170339

   Purpose

   GFPDel(UST)_EPACdDEPCD_mRFP biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

   Depositor

   Kees Jalink

   Article

   van der Krogt et al PLoS ONE. 2008 . 3(4):e1916.

   Insert

   GFPDel(UST)_EPACdDEPCD_mRFP (RAPGEF3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Deletion of DEP domain, Catalytically dead T781A …

   Promoter

   CMV

   Available since

   Oct. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. dCas-hHDAC3-R265P

   Plasmid

   #103786

   Purpose

   Expresses dCas9 fused to human HDAC3 with R265P mutation

   Depositor

   Zhaolan Zhou

   Article

   Kwon et al Nat Commun. 2017 May 12;8:15315. doi: 10

   Insert

   dCas9-HDAC3-R265P (HDAC3 Synthetic, S. pyogenes, Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Changed arginine 265 to proline

   Promoter

   EF1A

   Available since

   Dec. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. His6_MBP_FXR1P

   Plasmid

   #198694

   Purpose

   Expression of human FXR1 protein in E. coli

   Depositor

   Simpson Joseph

   Article

   Edwards et al Sci Rep. 2020 Sep 28;10(1):15858. doi: 1

   Insert

   FXR1P (FXR1 Human)
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. iTol2Amp-γ-crystallin:RFP

   Plasmid

   #108455

   Purpose

   This plamid can be used to recombine the iTol2-Amp-γ-crystallin:RFP into a BAC.

   Depositor

   Nadia Mercader Huber

   Article

   Sanchez-Iranzo et al Nat Commun. 2018 Jan 30;9(1):428. doi: 1

   Insert

   Tol2-Amp-y-cryst-mCherry
   

   Use

   Gateway

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFUGW-Pol2-ffLuc2-eGFP

   Plasmid

   #71394

   Purpose

   Lentiviral vector of luciferase-eGFP fusion gene driven by Pol2 promoter

   Depositor

   Glenn Merlino

   Article

   Day et al Pigment Cell Melanoma Res. 2009 Jun;22(3

   Insert

   Pol2-ffLuc2-eGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Pol2 (mouse RNA polymerase II gene promtoer)

   Available since

   Dec. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. CMV-FlicR1

   Plasmid

   #74142

   Purpose

   Mammalian expression of FlicR1: A bright red fluorescent genetically encoded voltage indicator

   Depositor

   Robert Campbell

   Article

   Abdelfattah et al J Neurosci. 2016 Feb 24;36(8):2458-72. d

   Insert

   FlicR1
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET11a_His-TEV-PLpro (nsp3) (SARS-CoV-2)

   Plasmid

   #169192

   Purpose

   To express SARS-CoV-2 Papain-like protease in E. coli

   Depositor

   John Diffley

   Article

   Lim et al Biochem J. 2021 Jul 16;478(13):2517-2531

   Insert

   6His-TEV-nsp3_E746-K1060 (pp1ab_E1564-K1878) (ORF1ab Synthetic, SARS-CoV-2)
   

   Use

   Unspecified

   Tags

   6His-TEV

   Expression

   Mutation

   Codon optimised for E. coli

   Promoter

   T7

   Available since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCI-ING3

   Plasmid

   #79070

   Purpose

   Expression of p47 ING3 in mammalian cells

   Depositor

   Karl Riabowol

   Insert

   ING3 (p47 ING3 isoform) (ING3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pLenti-mCherry-Cre-blast

    Plasmid

    #179390

    Purpose

    Lentiviral expression of Cre recombinase and mCherry reporter from CMV promoter (separated by P2A peptide), and blasticidin resistance gene for selection in mammalian cells.

    Depositor

    Moshe Szyf

    Article

    Sapozhnikov et al Nat Commun. 2021 Sep 29;12(1):5711. doi:

    Insert

    mCherry (P2A) Cre recombinase
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-HA-NLGN4 WT

    Plasmid

    #59320

    Purpose

    Expression plasmid of HA-tagged wildtype human Neuroligin 4

    Depositor

    Peter Scheiffele

    Article

    Chih et al Hum Mol Genet. 2004 Jul 15;13(14):1471-7

    Insert

    NLGN4 (NLGN4X Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-mRuby2

    Plasmid

    #40260

    Purpose

    RFP with robust performance in protein fusions and useful as FRET acceptor for Clover in a FRET pair that offers bright fluorescence, dynamic range, and photostability while limiting emissions overlap

    Depositor

    Michael Lin

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Has Service

    Cloning Grade DNA

    Insert

    mRuby2
    

    Use

    Tags

    His6 and Xpress

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE

    Available since

    Sept. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. Flag-p50(1-435)

    Plasmid

    #44747

    Depositor

    Gourisankar Ghosh

    Article

    Savinova et al Mol Cell. 2009 Jun 12;34(5):591-602. doi

    Insert

    p50 (Nfkb1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    AA 1-435

    Promoter

    Available since

    Aug. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pICH47732:FCP:NAT

    Plasmid

    #85984

    Purpose

    Golden Gate Level 1 cassette encoding a Nat selectable marker under the FCP promoter

    Depositor

    Thomas Mock

    Article

    Hopes et al Plant Methods. 2016 Nov 24;12:49. doi: 1

    Insert

    NAT
    

    Use

    Golden gate assembly

    Tags

    Expression

    Mutation

    Promoter

    FCP

    Available since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-CAG-FLEXFRT-ChR2(H134R)-mCherry

    Plasmid

    #75470

    Purpose

    Flp-dependent Chr2-mCherry encoded in an AAV vector for rAAV production and expression in mammalian cells

    Depositor

    Connie Cepko

    Article

    Tang et al Elife. 2016 May 20;5. pii: e15312. doi:

    Has Service

    AAV1

    Insert

    Reversed ChR2(H134R)-mCherry
    

    Use

    AAV and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCIBN(deltaNLS)-pmGFP

    Plasmid

    #26867

    Purpose

    Expresses CIBN(1-170, -NLS)-eGFP-CaaX fusion for use in light-inducible protein interaction modules for targeting to the plasma membrane

    Depositor

    Chandra Tucker

    Article

    Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

    Insert

    CIBN(deltaNLS)-pmGFP (CIB1 Mustard Weed)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    NLS sequences mutated

    Promoter

    CMV

    Available since

    Feb. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDGB3 alpha2 P35S:dCas9:SRDX:Tnos (GB1188)

    Plasmid

    #160608

    Purpose

    TU for the expression of the dCas9 with the repressor domain SRDX as a CT fusion (CRISPR tools)

    Depositor

    Diego Orzaez

    Article

    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

    Insert

    P35s:dCas9:SRDX:tNOS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    P35S

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. Ube2v2

    Plasmid

    #31430

    Depositor

    Rachel Klevit

    Article

    Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

    Insert

    Ubiquitin Conjugating Enzyme (UBE2V2 Human)
    

    Use

    Tags

    HA

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pLaNGo11-7

    Plasmid

    #126055

    Purpose

    A self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cell

    Depositor

    Ralf-Bernd Klösgen

    Article

    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

    Inserts

    chloroplastic FNR transit peptide
    ccdB cassette (BsaI)
    GFP11x7 (5 aa linker)
    

    Use

    Binary

    Tags

    GFP1-10

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. mCherry-CD36-C-10

    Plasmid

    #55011

    Purpose

    Localization: Membrane, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    CD36 (CD36 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge