162,109 results

1. 6xHis-SNAP-PI(4,5)P2 Probe

   Plasmid

   #211513

   Purpose

   For expression of recombinant biosensor for detection of Phosphatidylinositol-4,5-Bisphosphate (PI(4,5)P2) from bacteria

   Depositor

   Hannes Maib, David Murray

   Article

   Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

   Insert

   PLCdelta1-PH
   

   Use

   Tags

   6xhis-SNAP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. Flag-mPIAS1

   Plasmid

   #15206

   Depositor

   Ke Shuai

   Article

   Liu et al Proc Natl Acad Sci U S A. 1998 Sep 1. 95

   Insert

   PIAS1 (Pias1 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 13, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHR-UCOE-SFFV-EGFP

   Plasmid

   #188900

   Purpose

   Human lentiviral vector for expression of EGFP from a SFFV promoter with an upstream ubiquitous chromatin opening element

   Depositor

   Jonathan Weissman, Marco Jost

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   EGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. ABE8e-N108Q

   Plasmid

   #194157

   Purpose

   Mammalian expression of ABE8e-N108Q

   Depositor

   Dali Li

   Article

   Chen et al Nat Chem Biol. 2022 Oct 13. pii: 10.1038

   Insert

   TadA-8e (N108Q)-nSpCas9
   

   Use

   CRISPR

   Tags

   6*His

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. SPOP-V5 (BTB+BACK)

   Plasmid

   #169723

   Purpose

   Mammalian Expression of mouse SPOP-BTB+BACK (L186D, L190D, L193D, I217K, Y353E)

   Depositor

   Tanja Mittag

   Article

   Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

   Insert

   SPOP (Spop Mouse)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   L186D, L190D, L193D, I217K, Y353E

   Promoter

   CMV

   Available since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. LentiU6-hIL2RN gRNA_multi1-3-MS2-Puro

   Plasmid

   #192684

   Purpose

   Lentiviral expression of multi gRNAs targeting hIL1RN promoter to activate human IL1RN transcription

   Depositor

   Jeffrey Miner

   Article

   Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

   Insert

   Human IL1RN activating gRNAs #1,2,3 (IL1RN Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. mito-BFP

   Plasmid

   #49151

   Purpose

   visualization of mitochondria

   Depositor

   Gia Voeltz

   Article

   Friedman et al Science. 2011 Oct 21;334(6054):358-62. d

   Insert

   mitochondrial targeting signal (COX4 Budding Yeast)
   

   Use

   Tags

   BFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-N-Myc_WT USP7

   Plasmid

   #131242

   Purpose

   Mammalian expression of N-terminally Myc-tagged USP7

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

   Insert

   Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV enhancer + CMV promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. PGKp-GFP-YAP (S94A)

   Plasmid

   #174173

   Purpose

   Lentiviral vector to constitutively express TEAD-binding mutant YAP under control of the human PGK promoter.

   Depositor

   Rod Bremner

   Article

   Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610

   Insert

   YAP (YAP1 Human)
   

   Use

   Lentiviral

   Tags

   3x FLAG tag

   Expression

   Mammalian

   Mutation

   Serine 94 mutated to Alanine

   Promoter

   PGK

   Available since

   Aug. 19, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pm-iATPSnFR1.1

    Plasmid

    #102549

    Purpose

    membrane displayed ATP sensor for mammalian cells

    Depositor

    Baljit Khakh

    Article

    Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

    Insert

    iATPSnFR1.1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. rAAV-CAG::FLEX-rev:: hM4D-2a-GFP

    Plasmid

    #52536

    Purpose

    Co-expresses hM4D and a green fluorescent label from a Cre-dependent virus

    Depositor

    Scott Sternson

    Article

    Atasoy et al Nature. 2012 Aug 9;488(7410):172-7. doi:

    Has Service

    AAV1

    Insert

    hM4D-2a-GFP (CHRM4 Human, Synthetic)
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    CAG

    Available since

    June 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX-6P1-Vinculin tail

    Plasmid

    #107154

    Purpose

    bacterial expression

    Depositor

    Tina Izard

    Article

    Chinthalapudi et al J Cell Biol. 2014 Dec 8;207(5):643-56. d

    Insert

    Vinculin (VCL Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Ptac

    Available since

    March 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUltra-5HTP

    Plasmid

    #178042

    Purpose

    Express modified ScTrpRS/tRNA for genetic incorporation of 5HTP to TAG codon

    Depositor

    Han Xiao

    Article

    Chen et al Chem. 2020 Oct 8;6(10):2717-2727. doi: 1

    Inserts

    Modified ScTrpRS for 5HTP incoporation
    suppressor tRNA for 5HTP incorporation
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    T107C, P254T, C255A

    Promoter

    Available since

    Jan. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. TFORF3448

    Plasmid

    #144924

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    TP63 (TP63 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA4TO-5'UTR_long Emi1-24xGCN4_v4-kif18b-24xPP7

    Plasmid

    #74932

    Purpose

    Translation reporter

    Depositor

    Marvin Tanenbaum

    Article

    Yan et al Cell. 2016 May 5;165(4):976-89. doi: 10.

    Insert

    5'UTR_long Emi1-24xGCN4_v4-kif18b-24xPP7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. T7-VEE-OKS-iM

    Plasmid

    #58972

    Purpose

    RNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2, separated by 2A peptides followed by an IRES then c-myc and a second IRES and PuroR ORF

    Depositor

    Steven Dowdy

    Article

    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

    Inserts

    Oct4 (POU5F1 Human)
    Klf4 (KLF4 Human)
    Sox2 (SOX2 Human)
    cMyc (MYC Human)
    

    Use

    Venezuelan equine encephalitis (vee) virus rna re…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    26S subgenomic promoter and IRES

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pICE-FLAG-NAT10-siR-WT

    Plasmid

    #59365

    Purpose

    Plasmid for constitutive or doxycycline-inducible expression of wild-type human NAT10 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

    Depositor

    Steve Jackson

    Article

    Larrieu et al Science. 2014 May 2;344(6183):527-32. do

    Insert

    human NAT10 (NAT10 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Mutations to render cDNA resistant to a siRNA

    Promoter

    CMV-tet

    Available since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP-PSPC1-CT

    Plasmid

    #124374

    Purpose

    expresses EGFP-PSPC1-CT131 in mammalian cell

    Depositor

    Yuh-Shan Jou

    Article

    Lang et al Nat Commun. 2019 Dec 16;10(1):5716. doi:

    Insert

    PSPC1 CT (PSPC1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRc/RSV-m CBP-HA

    Plasmid

    #16701

    Depositor

    Richard Goodman

    Article

    Chrivia et al Nature. 1993 Oct 28. 365(6449):855-9.

    Insert

    CBP (Crebbp Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 29, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. OPRM1-DuET

    Plasmid

    #213361

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    OPRM1 (OPRM1 Human)
    

    Use

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    April 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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