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pGEX SHP-2(C)-SH2

Plasmid

#46497

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

SHP-2(C) (PTPN11 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 101-222

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pDONR223_PCCB_WT_V5

Plasmid

#82938

Purpose

Gateway Donor vector containing PCCB, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

PCCB (PCCB Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV.ZAC.GFAP.iAChSnFR

Plasmid

#137962

Purpose

Expresses iAChSnFR under GFAP promoter

Depositor

Loren Looger

Article

Borden et al bioRxiv 2020

Insert

iAChSnFR

Use

AAV

Expression

Mammalian

Promoter

GFAP

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/HaloTag7-Geminin(1/110)_P2A_HaloTag9-Cdt(30-120)

Plasmid

#169336

Purpose

Expression of the LT-Fucci(SA) in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

Fucci(SA) (GMNN, CDT1 Human)

Tags

HaloTag7-Geminin(1/110) and HaloTag9-Cdt(30-120)

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Available Since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCIBN-LacI-tagRFP-T

Plasmid

#103810

Purpose

'Localizer' construct that marks lacO arrays and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitment

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

CIBN-LacI-tagRFP-T (CIB1, lacI Mustard Weed, E. coli)

Expression

Mammalian

Mutation

LacI: C19T (silent, L7L), T264C (silent, A88A), C…

Promoter

CMV

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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mKO2-CDK4

Plasmid

#215065

Purpose

Expresses mKO2-CDK4 in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

CDK4 (CDK4 Human)

Use

Retroviral

Tags

mKO2

Expression

Mammalian

Promoter

LTR

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_SMAD4_p.P356R

Plasmid

#82769

Purpose

Gateway Donor vector containing SMAD4, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

SMAD4 (SMAD4 Human)

Use

Gateway entry vector

Mutation

P356R

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_POLR2I_WT_V5

Plasmid

#82955

Purpose

Gateway Donor vector containing POLR2I, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

POLR2I (POLR2I Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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PB-PE SpG

Plasmid

#179081

Purpose

NOTE: An improved version of this plasmid is now available. See Addgene plasmid #242072. All-in-one prime editor piggyBac transposon, SpG variant

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

Insert

SpCas9 SpG_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)

Use

CRISPR and Synthetic Biology; Piggybac transposon

Tags

SV40 NLS

Expression

Mammalian

Mutation

D1135L, S1136W, G1218K, E1219Q, R1335Q, T1337R

Promoter

CAG

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pU6-pegRNA-SNCA-A30P

Plasmid

#180016

Purpose

Transiently expressing a pegRNA to introduce SNCA-A30P mutation in human cells

Depositor

Dirk Hockemeyer

Article

Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

Insert

Prime editing pegRNA for SNCA-A30P (SNCA Synthetic)

Use

CRISPR

Expression

Mammalian

Promoter

Human U6

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBPK1520-SNCA-A30P-ng

Plasmid

#180018

Purpose

Transiently expressing a nicking sgRNA to facilitate the introduction of SNCA-A30P mutation in PE3 approach

Depositor

Dirk Hockemeyer

Article

Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

Insert

Prime editing ngRNA for SNCA-A30P (SNCA Human)

Use

CRISPR

Expression

Mammalian

Promoter

Human U6

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pGL3-pSMANTIS_1kb_KLF2mut 449,548,732,899,884 (All)

Plasmid

#194190

Purpose

Includes the promoter (1kb) of SMTS with 5 mutated KLF binding sites (positions 449,548,732,899,884)

Depositor

Ralf Brandes, Matthias Leisegang

Article

Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533

Insert

SMANTIS promoter (1kb) (SMANTIS Human)

Use

Luciferase

Mutation

5 mutated KLF binding sites (positions 449,548,73…

Promoter

SMANTIS promoter (1kb), mutated

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-pSMANTIS_1kb_KLF2mut 899

Plasmid

#194193

Purpose

Includes the promoter (1kb) of SMTS with mutated KLF binding sites (position 899)

Depositor

Ralf Brandes, Matthias Leisegang

Article

Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533

Insert

SMANTIS promoter (1kb) (SMANTIS Human)

Use

Luciferase

Mutation

mutated KLF binding sites (position 899)

Promoter

SMANTIS promoter (1kb), mutated

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pMBP-parallel1-GK5_v2

Plasmid

#134288

Purpose

Expresses GK5 (isoform 2) in bacteria

Depositor

Bruce Beutler

Article

Zhang et al Proc Natl Acad Sci U S A. 2017 Jun 27;11

Insert

Gk5 (Gk5 Mouse)

Tags

MBP

Expression

Bacterial

Mutation

mouse Gk5 isoform 2; 534 amino acids

Promoter

T7

Available Since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pCEFL GAL4dbd-KLF4 1-329

Plasmid

#140146

Purpose

GAL4-DNA binding domain fusion protein with the 329 N-terminal amino acids of human KLF4

Depositor

Ramiro Iglesias-Bartolome

Article

Yuan et al Nat Commun. 2020 Mar 19;11(1):1472. doi:

Insert

KLF4 Kruppel factor (KLF4 Human)

Tags

GAL4dbd

Expression

Mammalian

Mutation

Deleted amino acids 330 to 479

Promoter

EF1a

Available Since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223_RBM45_WT_V5

Plasmid

#82968

Purpose

Gateway Donor vector containing RBM45, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

RBM45 (RBM45 Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_STC2_WT_V5

Plasmid

#82957

Purpose

Gateway Donor vector containing STC2, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

STC2 (STC2 Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pCEFL GAL4dbd-KLF4 1-156

Plasmid

#140147

Purpose

GAL4-DNA binding domain fusion protein with the 156 N-terminal amino acids of human KLF4 (activation domain)

Depositor

Ramiro Iglesias-Bartolome

Article

Yuan et al Nat Commun. 2020 Mar 19;11(1):1472. doi:

Insert

KLF4 aa 1-156 (KLF4 Human)

Tags

GAL4dbd

Expression

Mammalian

Mutation

Deleted amino acids 157 to 479

Promoter

EF1a

Available Since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223_NOSIP_WT_V5

Plasmid

#82970

Purpose

Gateway Donor vector containing NOSIP, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

NOSIP (NOSIP Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PSMD9_WT_V5

Plasmid

#82950

Purpose

Gateway Donor vector containing PSMD9, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

PSMD9 (PSMD9 Human)

Use

Gateway entry vector

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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