164,802 results

1. pcDNA3.1-HA-RAD52-WT

   Plasmid

   #235676

   Purpose

   Expresses HA-tagged wild type human RAD52 in mammalian cells.

   Depositor

   Simon Powell

   Article

   Jalan et al Nat Commun. 2024 Sep 5;15(1):7776. doi:

   Insert

   RAD52 (RAD52 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   Available Since

   April 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLXRN-EGFP

   Plasmid

   #99207

   Purpose

   EGFP expressing bicistronic retroviral vector

   Depositor

   Peter Pedersen

   Insert

   EGFP
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   A Kozak sequence was incorporated to enhance tran…

   Promoter

   LTR

   Available Since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAVS1-A2UCOE-EF1a-HSV TK-P2A-H2-Kb SCT-T2A-mCD47-E2A-mCD55-F2A-neo-AAVS1

   Plasmid

   #205446

   Purpose

   human AAVS1-targeting plasmid for expression of HSV TK, H2-Kb SCT, mouse CD47, and mouse CD55

   Depositor

   Deepta Bhattacharya

   Article

   Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

   Insert

   HSV TK-H2-Kb SCT-mCD47-mCD55
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Sept. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. LRRK2_Halo_N_allele

   Plasmid

   #178142

   Purpose

   Donor vector for endogenous tagging of human LRRK2 at the N-terminus with halotag

   Depositor

   Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

   Insert

   Halotag (LRRK2 Human)
   

   Use

   Donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSL1145 (pSPIN, pBBR1 backbone, R*MmeI)

   Plasmid

   #160736

   Purpose

   Single-plasmid V. cholerae CAST, encodes all proteins, crRNA, and donor DNA. Non-targeting crRNA with BsaI sites for spacer cloning. Mini-tn has MmeI site in R end for Tn-seq. pBBR1 backbone.

   Depositor

   Samuel H. Sternberg

   Article

   Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

   Insert

   VchCAST proteins, crRNA, and donor DNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available Since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGL4.10-Claudin-1

   Plasmid

   #46387

   Purpose

   Claudin-1 promoter reporter assay

   Depositor

   David Mu

   Article

   Runkle et al J Biol Chem. 2012 Aug 17;287(34):28790-8

   Insert

   CLDN1 Promoter (CLDN1 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   CLDN1 promoter

   Available Since

   Aug. 2, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pAD-KPS12-Pman

   Plasmid

   #133832

   Purpose

   Fluorescent protein mKate(KPS12) variant for optimized expression in Bacillus mycoides; under control of mannose inducible promoter.

   Depositor

   Oscar Kuipers

   Article

   Yi et al Environ Microbiol Rep. 2018 Feb;10(1):57

   Insert

   mKate(KPS12)
   

   Use

   E.coli/bacillus spp. shuttle vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pman

   Available Since

   Nov. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHR_PGK_SNIPR_Hinge Notch SNIPR (LaG17)

   Plasmid

   #188380

   Purpose

   Lentiviral vector - constitutive expression of an anti-GFP SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a Gal4VP64 transcriptional factor

   Depositor

   Kole Roybal

   Article

   Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

   Insert

   PGK_antiGFP_CD8alpha-variant2-ECD_Notch1-TMD_Notch2-JMD_Gal4VP64
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti6-DEST PINK1-V5 WT

   Plasmid

   #13320

   Depositor

   Mark Cookson

   Article

   Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

   Insert

   PINK1 (PINK1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMOD_A1110

    Plasmid

    #91031

    Purpose

    Module A, Promoter: ZmUbi, Gene: TaCas9, Terminator: HSP

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    TaCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    ZmUbi

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pENTR-L1-PGK-PuroTK-L2

    Plasmid

    #141005

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    PuroTK
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP-N3/hArf6(WT)-EGFP

    Plasmid

    #79423

    Purpose

    Expresses C-terminally EGFP-tagged Arf6(WT) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

    Insert

    Arf6 (ARF6 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMPRAdonor2

    Plasmid

    #49353

    Purpose

    minP-luc2 donor plasmid for MPRA library construction

    Depositor

    Tarjei Mikkelsen

    Article

    Melnikov et al J Vis Exp. 2014 Aug 17;(90). doi: 10.379

    Insert

    luc2
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    minP

    Available Since

    Feb. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. AIO-Puro

    Plasmid

    #74630

    Purpose

    All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and Cas9-D10A nickase linked via 2A peptide with puromycin resistant marker to enhance efficient and accurate genome editing

    Depositor

    Steve Jackson

    Article

    Chiang et al Sci Rep. 2016 Apr 15;6:24356. doi: 10.10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Puromycin resistant marker

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available Since

    May 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-TRE-GFP-Blast

    Plasmid

    #135673

    Purpose

    Dox-inducible expression (TRE promoter) of GFP cDNA (control for other cDNAs), Blasticidin selection

    Depositor

    Florian Karreth

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAVS1-Tet-OsTIR1(WT)-V5

    Plasmid

    #158663

    Purpose

    Tet-OsTIR1(WT)-V5 donor for integration at the AAVS1 locus

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    Tet-OsTIR1(WT) AAVS1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSEED-sfGFP

    Plasmid

    #219639

    Purpose

    Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in sfGFP using the SEED/Harvest method

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    sfGFP SEED Cassette
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. Lck-GFP

    Plasmid

    #61099

    Purpose

    Expression of a membrane-targeted form of eGFP

    Depositor

    Steven Green

    Article

    Benediktsson et al J Neurosci Methods. 2005 Jan 30;141(1):4

    Insert

    Lck-GFP
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. mRNA-CleanCap-NanoLuc

    Plasmid

    #188247

    Purpose

    Template plasmid to transcribe mRNA encoding NanoLuc

    Depositor

    Howard Chang

    Article

    Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

    Insert

    NanoLuc
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available Since

    Sept. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. DR274

    Plasmid

    #42250

    Purpose

    guide RNA (gRNA) expression vector used to create a gRNA to a specific sequence; uses T7 promoter for in vitro transcription

    Depositor

    Keith Joung

    Article

    Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR; Zebrafish expression

    Tags

    Expression

    Worm

    Mutation

    Promoter

    T7

    Available Since

    Jan. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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21. pLenti_CMV_CapChR2-GFP

    Plasmid

    #224357

    Purpose

    Expresses CapChR2-GFP in mammalian cells

    Depositor

    Adam Cohen

    Article

    Hayward et al Cell Calcium. 2024 Dec 5;125:102983. doi

    Insert

    CapChR2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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22. HCT159_pAAV-eHGT_3Xcore_1137m-minBGprom-iCre297T-WPRE3-bGHpA

    Plasmid

    #233558

    Purpose

    Direct-expressing iCre297T AAV virus

    Depositor

    Allen Institute for Brain Science, Tanya Daigle

    Article

    Kussick et al Cell Rep. 2025 Jun 24;44(6):115730. doi:

    Insert

    iCre297T
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    minBglobin

    Available Since

    June 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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23. pWALIUM-intron 3x UAS attB

    Plasmid

    #206360

    Purpose

    includes two standard UAS cassettes and one UAS cassette designed to co-express shRNAs/shRNA clusters and prodein coding sequences

    Depositor

    Erdem Bangi

    Article

    Teague et al PLoS Genet. 2023 Jun 2;19(6):e1010792. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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24. M13KO7-ΔM13-ΔgeneIII-ΔgeneVI

    Plasmid

    #80840

    Purpose

    Modified helper phage

    Depositor

    Mark Isalan

    Article

    Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

    Inserts

    M13 gene II
    M13 gene X
    M13 gene V
    M13 gene VII
    M13 gene IX
    M13 gene VIII
    M13 gene I
    M13 gene XI
    M13 gene IV
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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25. pcDNA3-FLAG-mTET2

    Plasmid

    #89735

    Purpose

    Expresses wildtype mouse TET2 in mammalian cells

    Depositor

    Yue Xiong

    Article

    Nakagawa et al Mol Cell. 2015 Jan 22;57(2):247-60. doi:

    Insert

    TET2 (Tet2 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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26. pX330-CANX

    Plasmid

    #227279

    Purpose

    Expresses SpCas9 and a sgRNA targeting the C-terminus of CANX for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of CANX (CANX Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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27. pAAVS1-P-CAG-GFP

    Plasmid

    #80491

    Purpose

    donor vector for AAVS1 targeting (puromycin selection) and constitutive GFP expression

    Depositor

    Knut Woltjen

    Article

    Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

    Insert

    CAG-GFP
    

    Use

    Donor vector (human)

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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28. pCK123 pDESTattB_exorh:mCerulean

    Plasmid

    #213512

    Purpose

    attB-containing pDEST plasmid, for use in Multisite Gateway reaction

    Depositor

    Christian Mosimann

    Article

    Lalonde et al Sci Adv. 2024 Jun 7;10(23):eadn6603. doi

    Type

    Empty backbone

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. NA20 LN16

    Plasmid

    #101870

    Purpose

    Expresses HIV Env gene when transfected into 293-T cells for production of pseudovirus

    Depositor

    Paul Clapham

    Article

    Peters et al J Virol. 2004 Jul;78(13):6915-26.

    Insert

    Env (env )
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

30. pPB_TRE3G_scFV-sfGFP-Dnmt3a3lCD

    Plasmid

    #235576

    Purpose

    Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector Dnmt3a3L fused with scFV to programme DNA methylation

    Depositor

    Jamie Hackett

    Article

    Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

    Insert

    Dnmt3a-Dnmt3L (Dnmt3a, Dnmt3l Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge