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SypHer mt

Plasmid

#48251

Purpose

pH sensor, targeted to mitochondria

Depositor

Nicolas Demaurex

Article

Poburko et al J Biol Chem. 2011 Apr 1;286(13):11672-84

Insert

SypHer

Tags

2 mitochondrial targeting sequences

Expression

Mammalian

Mutation

C199S

Promoter

CMV

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-Lck-mTagBFP2

Plasmid

#234537

Purpose

to label the membrane of cells with a fluorescent protein (mTagBFP2)

Depositor

Hajime Hirase

Article

Wang et al Anat Sci Int. 2025 Jun 27. doi: 10.1007/

Insert

Lck-mTagBFP2

Use

AAV

Mutation

None

Promoter

Short CAG

Available Since

June 11, 2025

Availability

Academic Institutions and Nonprofits only

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pET15-UbE2D1

Plasmid

#15782

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2D1 (UBE2D1 Human)

Tags

6x His, T7 tag, and Xpress tag (enterokinase clea…

Expression

Bacterial

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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Cortactin-pmCherryC1

Plasmid

#27676

Depositor

Christien Merrifield

Article

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Insert

Cortactin (Cttn Mouse)

Tags

mCherry

Expression

Mammalian

Available Since

May 4, 2011

Availability

Academic Institutions and Nonprofits only

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NFAT luciferase reporter

Plasmid

#10959

Depositor

Toren Finkel

Article

Ichida et al J Biol Chem. 2001 Feb 2. 276(5):3524-30.

Insert

IL-2 promoter (IL2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

Jan. 6, 2006

Availability

Academic Institutions and Nonprofits only

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HA Raptor

Plasmid

#8513

Depositor

David Sabatini

Article

Kim et al Cell 2002 Jul 26;110(2):163-75.

Insert

Raptor (RPTOR Human)

Tags

ha

Expression

Mammalian

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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PH-Akt-GFP

Plasmid

#51465

Purpose

Is a biosensor for PIP3/PI(3,4)P2

Depositor

Tamas Balla

Article

Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

Insert

AKT (aa 1–164)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 26, 2014

Availability

Academic Institutions and Nonprofits only

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pQE30-His-T7RNAP

Plasmid

#124138

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

T7 RNA polymerase (T7p07 Enterobacteria phage T7)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pDC180

Plasmid

#218673

Purpose

Expresses rat ⍺SNAP in bacteria

Depositor

Axel Brunger

Article

Leitz et al Nat Protoc. 2024 Jul 2. doi: 10.1038/s41

Insert

rat ⍺SNAP (Napa Rat)

Tags

6 his

Expression

Bacterial

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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PP74

Plasmid

#78944

Purpose

Supplemental Figure 3. Integration Site 1.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

Integration Site 1

Expression

Yeast

Available Since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pJAT74

Plasmid

#204296

Purpose

pJAT plasmid with multiple cloning site for introducing custom payload. One U6 promoter.

Depositor

David Stern

Article

Stern et al bioRxiv 2023.06.17.545412

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-DIO-Opto-cytTrkB(E281A)-HA

Plasmid

#180590

Purpose

Opto-cytTrkB

Depositor

Won Do Heo

Article

Hong J et al J Mol Biol. 2020 Feb 14;432(4):815-827.

Insert

TrkB

Use

AAV

Mutation

PHR(E281A)

Available Since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCVII-E2Crimson

Plasmid

#162756

Purpose

Retroviral (MSCV) expression of E2-Crimson fluorescent protein

Depositor

Maki Nakayama

Article

Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

Insert

E2-Crimson

Use

Retroviral

Expression

Mammalian

Available Since

Dec. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pQE30-His-CKM

Plasmid

#124134

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Creatine kinase (CKM Chicken)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pEBFP2

Plasmid

#165831

Purpose

EBFP2blue fluorescent protein CDS

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

EBFP2

Expression

Insect

Available Since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pICH-roGFP2-synORP1

Plasmid

#191680

Purpose

Ti plasmid pICH86988 harbouring roGFP2-synORP1 for H2O2 detection in plant cells.

Depositor

Philip Mullineaux

Article

Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

Insert

rredox(roGFP2-synORP1

Expression

Plant

Promoter

CaMV 35S

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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pOZ-N-FH RAP80

Plasmid

#27501

Depositor

Roger Greenberg

Article

Patterson-Fortin et al J Biol Chem. 2010 Oct 1. 285(40):30971-8

Insert

RAP80 (UIMC1 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Available Since

March 1, 2011

Availability

Academic Institutions and Nonprofits only

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TRE-mCherry

Plasmid

#92202

Purpose

Fluorescent protein reporter construct

Depositor

Alice Ting

Article

Wang et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

Insert

mCherry

Use

AAV

Expression

Mammalian

Promoter

TRE

Available Since

June 16, 2017

Availability

Academic Institutions and Nonprofits only

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pEF-1a_mScarlet_IRES_BSD_WPRE

Plasmid

#251222

Purpose

Lentiviral transfer plasmid for the generation of EF1a driven mScarlet expressing cells

Depositor

Niels Geijsen

Article

Bot et al Commun Biol. 2026 Jan 19;9(1):233. doi:

Insert

mScarlet

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a

Available Since

March 19, 2026

Availability

Academic Institutions and Nonprofits only

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pDONR223_NKX2-1

Plasmid

#221472

Purpose

Gateway cloning of NKX2-1 short isoform overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Gateway donor vector

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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Cyto_K0

Plasmid

#133864

Purpose

Expression of cytoplasmic Ribosome non-stalling reporter

Depositor

Yihong Ye

Article

Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

Insert

Cyto_K0

Tags

EGFP and RFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pBbE6k-RFP

Plasmid

#35288

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

LlacO-1

Available Since

Feb. 16, 2012

Availability

Academic Institutions and Nonprofits only

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pME pHluorin2

Plasmid

#73794

Purpose

ratiometric pHluorin2 sequence in a middle entry clone

Depositor

David Raible

Article

Stawicki et al Dis Model Mech. 2014 Jul;7(7):847-56. do

Insert

ratiometric pHluorin2

Use

Middle entry clone

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pCZGY2141

Plasmid

#87757

Purpose

Pcol-19-PH::miniSOG, expression of membrane targeted PH-miniSOG in epidermis.

Depositor

Andrew Chisholm

Article

Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

Insert

Pcol-19-PH-miniSOG

Tags

PH

Expression

Worm

Promoter

Pcol-19

Available Since

March 29, 2017

Availability

Academic Institutions and Nonprofits only

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pCGN-Oct-1

Plasmid

#53308

Purpose

plasmid contains POU2F1 (Oct-1) gene with HA-tag

Depositor

Winship Herr

Article

Tanaka et al Cell. 1990 Feb 9;60(3):375-86.

Insert

Oct-1 (POU2F1 Human)

Tags

HA-tag

Expression

Mammalian

Promoter

CMV

Available Since

May 28, 2014

Availability

Academic Institutions and Nonprofits only

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pUC18-GFP

Plasmid

#220495

Purpose

To generate substrates to monitor Ku overloading and its impact on transcription at the DNA ends vicinity.

Depositor

Sébastien Britton

Article

Bossaert et al bioRxiv [Preprint]. 2024 Jan 4:2024.01.0

Insert

EGFP

Expression

Mammalian

Promoter

SV40

Available Since

July 3, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pEF1a-IRES-Neo

Plasmid

#28019

Depositor

Thomas Zwaka

Article

Dejosez et al Genes Dev. 2010 Jul 15. 24(14):1479-84.

Type

Empty backbone

Expression

Mammalian

Available Since

May 17, 2011

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-Lck-mStayGold

Plasmid

#234539

Purpose

to label the membrane of cells with a fluorescent protein (mStayGold)

Depositor

Hajime Hirase

Article

Wang et al Anat Sci Int. 2025 Jun 27. doi: 10.1007/

Insert

Lck-mStayGold(Ando)

Use

AAV

Mutation

None

Promoter

Short CAG

Available Since

June 11, 2025

Availability

Academic Institutions and Nonprofits only

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V5-BS2-NLS (Nucleoplasmin)

Plasmid

#220342

Purpose

Mammalian expression vector BS2 in nucleus

Depositor

Bryan Dickinson

Article

Pani et al Nat Chem. 2024 Apr 9. doi: 10.1038/s4155

Insert

BS2

Tags

NLS (Nucleus localization sequence) and V5 tag

Expression

Mammalian

Promoter

CMV

Available Since

June 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pCAG mito-RCaMP1h

Plasmid

#105013

Purpose

mitochondrial calcium sensor

Depositor

Franck Polleux

Article

Hirabayashi et al Science. 2017 Nov 3;358(6363):623-630. d

Insert

RCaMP1h

Expression

Mammalian

Promoter

pCAG

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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