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pWT-IFl_WT-IFr

Plasmid

#192207

Purpose

Wildtype I-F CRISPR Leader and a CRISPR locus with two wildtype I-F repeats, for integration assays.

Depositor

Blake Wiedenheft

Publication

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Wildtype I-F Leader and wildtype I-F Locus DNA

Expression

Bacterial

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pScr-IFl_WT-IFr

Plasmid

#192208

Purpose

Scrambled I-F CRISPR Leader and a CRISPR locus with two wildtype I-F repeats, for integration assays.

Depositor

Blake Wiedenheft

Publication

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Scrambled I-F Leader and wildtype I-F Locus DNA

Expression

Bacterial

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCon-IFl_Con-IFr

Plasmid

#192209

Purpose

Consensus I-F CRISPR Leader and a CRISPR locus with two consensus I-F repeats, for integration assays.

Depositor

Blake Wiedenheft

Publication

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Consensus I-F Leader and Consensus I-F Locus DNA

Expression

Bacterial

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCon-IFl_Con-IEr

Plasmid

#192210

Purpose

Consensus I-F CRISPR Leader and a CRISPR locus with two consensus I-E repeats, for integration assays.

Depositor

Blake Wiedenheft

Publication

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Consensus I-F Leader and Consensus I-E Locus DNA

Expression

Bacterial

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCon-IFl_Con-ICr

Plasmid

#192211

Purpose

Consensus I-F CRISPR Leader and a CRISPR locus with two consensus I-C repeats, for integration assays.

Depositor

Blake Wiedenheft

Publication

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Consensus I-F Leader and Consensus I-C Locus DNA

Expression

Bacterial

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCon-IFl_Con-IIAr

Plasmid

#192212

Purpose

Consensus I-F CRISPR Leader and a CRISPR locus with two consensus II-A repeats, for integration assays.

Depositor

Blake Wiedenheft

Publication

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Consensus I-F Leader and Consensus II-A Locus DNA

Expression

Bacterial

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCon-IEl_Con-IEr

Plasmid

#192213

Purpose

Consensus I-E CRISPR Leader and a CRISPR locus with two consensus I-E repeats, for integration assays.

Depositor

Blake Wiedenheft

Publication

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Consensus I-E Leader and Consensus I-E Locus DNA

Expression

Bacterial

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCon-ICl_Con-ICr

Plasmid

#192214

Purpose

Consensus I-C CRISPR Leader and a CRISPR locus with two consensus I-C repeats, for integration assays.

Depositor

Blake Wiedenheft

Publication

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Consensus I-C Leader and Consensus I-C Locus DNA

Expression

Bacterial

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pCon-IIAl_Con-IIAr

Plasmid

#192215

Purpose

Consensus II-A CRISPR Leader and a CRISPR locus with two consensus II-A repeats, for integration assays.

Depositor

Blake Wiedenheft

Publication

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

Consensus II-A Leader and Consensus II-A Locus DNA

Expression

Bacterial

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pX1-IFl_Con-IFr

Plasmid

#192216

Purpose

Mutant I-F CRISPR Leader and a CRISPR locus with two consensus I-F repeats, for integration assays.

Depositor

Blake Wiedenheft

Publication

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

I-F leader and I-F repeat construct not studied further in this paper (X1)

Expression

Bacterial

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pX2-IFl_Con-IFr

Plasmid

#192217

Purpose

Mutant I-F CRISPR Leader and a CRISPR locus with two consensus I-F repeats, for integration assays.

Depositor

Blake Wiedenheft

Publication

Santiago-Frangos et al Nat Struct Mol Biol. 2023 Nov;30(11):167

Insert

I-F leader and I-F repeat construct not studied further in this paper (X2)

Expression

Bacterial

Available since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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VEGF120TAG

Plasmid

#200946

Purpose

Encodes recombinant mouse VEGF120 when coupled with amber stop codon suppression by non-canonical amino acid incorporation

Depositor

Elizabeth Kirby

Publication

Miller et al Mol Psychiatry. 2024 Nov 11. doi: 10.103

Inserts

VEGF120TAG (Vegfa Mouse)
hPGK-driven Puromycin resistance

Use

Lentiviral

Tags

6x HIS

Mutation

amber stop codon 101 bp from ATG

Promoter

Ef1a and hPGK

Available since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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VEGF164TAG

Plasmid

#200947

Purpose

Encodes recombinant mouse VEGF164 when coupled with amber stop codon suppression by non-canonical amino acid incorporation

Depositor

Elizabeth Kirby

Publication

Miller et al Mol Psychiatry. 2024 Nov 11. doi: 10.103

Inserts

VEGF164TAG (Vegfa Mouse)
hPGK-driven Puromycin resistance

Use

Lentiviral

Tags

6x HIS

Mutation

amber stop codon 101 bp from ATG

Promoter

Ef1a and hPGK

Available since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-syn-mCre

Plasmid

#178515

Purpose

AAV vector for mCre recombinase

Depositor

Ian Wickersham

Publication

Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

Insert

mCre

Use

AAV and Cre/Lox

Expression

Mammalian

Available since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLV-TTBG

Plasmid

#115232

Purpose

Lentiviral transfer plasmid for expression of SAD B19 G and mTagBFP2

Depositor

Ian Wickersham

Publication

Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

Insert

mTagBFP2 and rabies virus SAD B19 strain glycoprotein gene (B19G)

Expression

Mammalian

Available since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-TTBL

Plasmid

#115233

Purpose

Lentiviral transfer plasmid for expression of SAD B19 L and mTagBFP2

Depositor

Ian Wickersham

Publication

Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

Insert

mTagBFP2 and rabies virus SAD B19 strain polymerase gene (B19L)

Expression

Mammalian

Available since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pBbB8k-csg-NbSARS

Plasmid

#171703

Purpose

Arabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA is fused to a cross-reactive nanobody targeting the spike protein of betacoronaviruses via a flexible linker.

Depositor

Neel Joshi

Publication

Birnbaum et al ACS ES T Water. 2022 Nov 11;2(11):1836-1

Insert

csgBACEFG, with nanobody fused to CsgA

Use

Synthetic Biology

Expression

Bacterial

Available since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pSECC-sgSIK1-B

Plasmid

#138666

Purpose

Expresses a mouse SIK1-targeting sgRNA, Cas9, and Cre-recombinase

Depositor

Reuben Shaw

Publication

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgSIK1 mouse (Sik1 Mouse)

Use

Lentiviral

Promoter

hU6

Available since

March 20, 2020

Availability

Academic Institutions and Nonprofits only

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pSECC-sgSIK3-A

Plasmid

#138672

Purpose

Expresses a mouse SIK3-targeting sgRNA, Cas9, and Cre-recombinase

Depositor

Reuben Shaw

Publication

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgSIK3 mouse (Sik3 Mouse)

Use

Lentiviral

Promoter

hU6

Available since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pSECC-sgSIK2-A

Plasmid

#138670

Purpose

Expresses a mouse SIK2-targeting sgRNA, Cas9, and Cre-recombinase

Depositor

Reuben Shaw

Publication

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgSIK2 mouse (Sik2 Mouse)

Use

Lentiviral

Promoter

hU6

Available since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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