We narrowed to 15,599 results for: LEA

1. pAGT6471

   Plasmid

   #219428

   Purpose

   Module of intronized NLS-SpCas9-NLS for N-terminal and C-terminal tag fusions (AGGT_N-SpCas9i-N_TTCG)

   Depositor

   Tom Schreiber

   Article

   Schreiber et al Mol Plant. 2024 Mar 22:S1674-2052(24)000

   Insert

   AGGT_NLS-SpCas9i-NLS(no stop)_TTCG
   

   Use

   Moclo compatible level 0 module

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti GW V5 Eco/Dam mse EMD

   Plasmid

   #182675

   Purpose

   Lentiviral eukaryotic expression vector with E.Coli Dam methylation fused to mouse Emerin. Used in DamID experiments to methylate DNA that interacts in the proximity of Emerin.

   Depositor

   Bas van Steensel

   Article

   Amendola et al EMBO Rep. 2015 May;16(5):610-7. doi: 10.

   Insert

   Emerin (Emd Mouse)
   

   Use

   Lentiviral

   Tags

   E. Coli Dam and V5

   Expression

   Mammalian

   Mutation

   Promoter

   HSP

   Available since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRSFDuet-1_VNp-hGH

   Plasmid

   #182411

   Purpose

   Bacterial expression of Vesicle Nucleating peptide-human growth hormone fusion

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   VNp-hGH (GH1 Human)
   

   Use

   Tags

   Vesicle Nucleating peptide (VNp)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTER E2F1shRNA human

   Plasmid

   #66883

   Purpose

   Doxycycline-regulated mammalian expression vector for expressing shRNA against E2F1

   Depositor

   Maria Alvarado-Kristensson

   Article

   Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

   Insert

   E2F transcription factor 1 (E2F1 Human)
   

   Use

   RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1 Tet

   Available since

   June 25, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pCMV_RBMX-eA3A-UdgX-nCas9

   Plasmid

   #163546

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   RBMX-eA3A-UdgX-nCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)

   Promoter

   Available since

   Dec. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. PD-1-miSFIT-Ce67

   Plasmid

   #124680

   Purpose

   Tuning expression of PD-1

   Depositor

   Tudor Fulga

   Article

   Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

   Insert

   PD-1 (PDCD1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   minimal CMV

   Available since

   June 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHAGE2-TetOminiCMV-Sox2

   Plasmid

   #136612

   Purpose

   Dox-inducible lentiviral vector expressing mouse Sox2

   Depositor

   Hans Schöler

   Article

   Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

   Insert

   Sox2 (Sox2 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tetO-miniCMV (dox-inducible)

   Available since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGL3-Basic-hCYP26A1-FL- luciferase

   Plasmid

   #135566

   Purpose

   Full Length promoter of the human CYP26A1 gene in pGL3-Basic-luciferase

   Depositor

   Catharine Ross

   Article

   Zolfaghari et al J Cell Biochem. 2014 Oct;115(10):1740-51

   Insert

   Full-length promoter of human retinoic acid receptor-beta gene
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   hHuman CYP26A1 gene promoter

   Available since

   Jan. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. BPK2797

   Plasmid

   #72251

   Purpose

   Human expression plasmid for SpCas9-VRQR variant: CMV-T7-humanSpCas9-VRQR(D1135V, G1218R, R1335Q, T1337R)-NLS-3xFLAG

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9 VRQR(D1135V/G1218R/R1335Q/T1337R)-NLS-3xFlag
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Mammalian

   Mutation

   D1135V, G1218R, R1335Q, and T1337R

   Promoter

   CMV

   Available since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV_eA3A T31A-dCas9

    Plasmid

    #163537

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    eA3A T31A-dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    dCas9 (D10A, H840A)eA3A T31A

    Promoter

    Available since

    Dec. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGAPDH::H2B-mScarlet

    Plasmid

    #186764

    Purpose

    A plasmid containing planarian H2B fused to a planarian codon-optimized mScarlet, driven by the planarian GAPDH promoter.

    Depositor

    Bo Wang

    Article

    Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

    Insert

    H2B-mScarlet
    

    Use

    Overexpression

    Tags

    H2B

    Expression

    Mutation

    Promoter

    GAPDH

    Available since

    Oct. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHAGE2-TetOminiCMV-cMyc

    Plasmid

    #136614

    Purpose

    Dox-inducible lentiviral vector expressing mouse cMyc

    Depositor

    Hans Schöler

    Article

    Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

    Insert

    cMyc (Myc Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tetO-miniCMV (dox-inducible)

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDR366 RNF2

    Plasmid

    #47509

    Purpose

    human gRNA expression vector targeting RNF2

    Depositor

    Keith Joung

    Article

    Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

    Insert

    gRNA-RNF2 (RNF2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pENeGFP PCNAL2 pc653

    Plasmid

    #167564

    Purpose

    The GFP-PCNAL2 fusion protein contains a SV40 nuclear localization signal at the NH2 terminus followed by an enhanced mutant GFP gene that is fused to the human PCNA by a linker.

    Depositor

    Cristina Cardoso

    Article

    Leonhardt et al J Cell Biol. 2000 Apr 17;149(2):271-80.

    Insert

    Proliferating Cell Nuclear Antigen (PCNA Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLV-EF1a-V5-Lem2

    Plasmid

    #120245

    Purpose

    Expresses V5-tagged Lem2 in lentiviral vector

    Depositor

    Larry Gerace

    Article

    Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

    Insert

    Lem2 (Lemd2 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEmpty

    Plasmid

    #214051

    Purpose

    Empty bacterial expression vector with CloDF13 (CDF) origin of replication; used as a negative control in the Haliangium ochraceum type III CRISPR-Cas system

    Depositor

    Raymond Staals

    Article

    Steens et al Science. 2024 Feb 2;383(6682):512-519. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-CD81-(TEV)-FKBP-Myc

    Plasmid

    #177908

    Purpose

    human CD81-TEV cleavage site-FKBP-Myc tag

    Depositor

    Masaharu Somiya

    Article

    Somiya et al Mol Pharm. 2022 May 20. doi: 10.1021/acs

    Insert

    human CD81 (CD81 Human, Synthetic)
    

    Use

    Tags

    FKBP, Myc tag, and TEV protease cleavage site

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV6-G1-ATP8-Myc-FLAG (G418)

    Plasmid

    #86850

    Purpose

    mammalian expression of ATP8 with mitochondrial targeting sequence, Myc, and FLAG tags

    Depositor

    Matthew O'Connor

    Article

    Boominathan et al Nucleic Acids Res. 2016 Nov 2;44(19):934

    Insert

    ATP8 (ATP8 Human)
    

    Use

    Tags

    ATP5G1 (G1) (MQTAGALFISPALIRCCTRGLIRPVSASFLNS PVN…

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    CMV

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pClneoMyc human MST2

    Plasmid

    #37022

    Depositor

    Yutaka Hata

    Article

    Hirabayashi et al Oncogene. 2008 Jul 17;27(31):4281-92. Ep

    Insert

    MST2 (STK3 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 22, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pVC299 VEGF Site#2

    Plasmid

    #47506

    Purpose

    human gRNA expression vector targeting VEGF

    Depositor

    Keith Joung

    Article

    Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

    Insert

    gRNA-VEGF site 2 (VEGFA Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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