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pRC/CMV-TYK2P1104A-VSV

Plasmid

#139350

Purpose

Expression of the TYK2-P1104A variant, rs34536443

Depositor

Sandra Pellegrini

Article

Li et al J Immunol. 2013 Mar 1;190(5):2335-44. do

Insert

TYK2 cDNA with 267nt of 5' UTR (TYK2 Human)

Tags

VSV

Expression

Mammalian

Mutation

V362F-P1104A

Promoter

CMV

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-IP3KA

Plasmid

#133582

Purpose

Expresses IP3KA GFP tagged

Depositor

Christophe Erneux

Article

Dewaste et al Biochem J. 2003 Aug 15;374(Pt 1):41-9.

Insert

Human inositol 1,4,5-trisphosphate 3-kinase A GFP tagged (ITPKA Human)

Tags

GFP

Expression

Mammalian

Available Since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only
pGEX-6P-1-INTS3-C

Plasmid

#128417

Purpose

Express INTS3 C-termini (514-1042 aa) in E. coli with a GST tag (N-terminal)

Depositor

Yuliang Wu

Article

Vidhyasagar et al Biochem J. 2018 Jan 2;475(1):45-60. doi:

Insert

INTS3 (INTS3 Human)

Tags

GST

Expression

Bacterial

Mutation

INTS3 C-terminal region (514-1042 aa)

Promoter

Tac promoter

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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PB09-TRE-ZNF787_G379A

Plasmid

#117320

Purpose

Overexpression of ZNF787

Depositor

Volker Busskamp

Article

Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

Insert

ZNF787 (ZNF787 Human)

Use

Transposon-mediated integration

Expression

Mammalian

Mutation

G379A

Promoter

Tet-inducible promoter

Available Since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-Nup35-mut

Plasmid

#87341

Purpose

To express human Nup35 in mammalian cells. It contains synonymous mutations that are not targeted by shRNA

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

NUP35 (NUP35 Human)

Tags

EGFP

Expression

Mammalian

Mutation

shRNA resistant changes

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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E1b-GFP-Tol2-Gateway dre SE-zic2a P

Plasmid

#89385

Purpose

Vector for reporter assay of one zebrafish super-enhancer region

Depositor

Alena Shkumatava

Article

Perez-Rico et al Genome Res. 2017 Feb;27(2):259-268. doi:

Insert

zic2a (zic2a Zebrafish)

Available Since

April 7, 2017

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmCaf40

Plasmid

#79251

Purpose

Expresses EGFP-tagged DmCaf40 in S2 cells

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmCaf40 (Rcd-1 Fly)

Use

Schneider 2 cell expression

Tags

EGFP

Promoter

Ac5 promoter

Available Since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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MO70-N-Flag-hCRACR2A-isoform-a-Q604L

Plasmid

#79595

Purpose

Transient and retroviral expression

Depositor

Yousang Gwack

Article

Srikanth et al Sci Signal. 2016 Mar 22;9(420):ra31. doi

Insert

CRACR2A-isoform-a (CRACR2A Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Q604L

Promoter

CMV

Available Since

June 29, 2016

Availability

Academic Institutions and Nonprofits only
MO70-N-Flag-hCRACR2A-isoform-a-T559N

Plasmid

#79594

Purpose

Transient and retroviral expression

Depositor

Yousang Gwack

Article

Srikanth et al Sci Signal. 2016 Mar 22;9(420):ra31. doi

Insert

CRACR2A-isoform-a (CRACR2A Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

T559N

Promoter

CMV

Available Since

June 29, 2016

Availability

Academic Institutions and Nonprofits only
pCMV5-Flag-Ago2-FDM1

Plasmid

#72208

Purpose

Mammalian expression of aa 1-372 of human Ago2

Depositor

Mien-Chie Hung

Article

Shen et al Nature. 2013 May 16;497(7449):383-7. doi

Insert

argonaute RISC catalytic component 2 (AGO2 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Δ 373-860

Promoter

CMV

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only
pCMV5-Flag-Ago2-FDM2

Plasmid

#72209

Purpose

Mammalian expression of aa 1-517 of human Ago2

Depositor

Mien-Chie Hung

Article

Shen et al Nature. 2013 May 16;497(7449):383-7. doi

Insert

argonaute RISC catalytic component 2 (AGO2 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Δ 518-860

Promoter

CMV

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only
pES005 (pTet-qacR wt/pQacA-deGFP)

Plasmid

#69034

Purpose

reporter with wild-type qacR on single plasmid

Depositor

Richard Murray

Article

de Los Santos et al ACS Synth Biol. 2015 Aug 19.

Inserts

qacR (qacR Synthetic)
deGFP

Tags

His6 tag

Expression

Bacterial

Promoter

pQacA and pTet

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

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FLAG-HA-BLMP-1-pcDNA3.1-

Plasmid

#52512

Purpose

expresses C. elegans BLMP-1 in mammalian cells with FLAG-HA tag at N-terminus

Depositor

Adam Antebi

Article

Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

Insert

Blmp-1 (blmp-1 Nematode)

Tags

FLAG-HA

Expression

Mammalian

Mutation

N75S mutation compared GenBank reference sequence…

Promoter

CMV

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pSR29

Plasmid

#69255

Purpose

pelt-2::CRE for integration on ttTi14024, Chr X

Depositor

Sander van den Heuvel

Article

Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

Insert

Cre recombinase

Use

Cre/Lox; Mossci

Expression

Worm

Mutation

codon-optimzed index 1.0, tbb-2 UTR is in wrong o…

Promoter

elt-2

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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6his R.3-AR GUSpET28a+/pLysS

Plasmid

#61157

Purpose

N-terminal His tagged round 3 aldehyde-resistant GUS expression vector

Depositor

Andrew Ellington, Ichiro Matsumura

Article

Matsumura et al Nat Biotechnol. 1999 Jul;17(7):696-701.

Insert

GUSAR

Tags

6x His

Expression

Bacterial

Mutation

N66D, D151N, A219V, I396T, T480A, Q498R, D508E an…

Promoter

T7

Available Since

March 17, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-myc-AP1S3 F4C

Plasmid

#58293

Purpose

mammalian expression of AP1S3 F4C mutant

Depositor

Francesca Capon

Article

Setta-Kaffetzi et al Am J Hum Genet. 2014 May 1;94(5):790-7.

Insert

AP1S3 (AP1S3 Human)

Tags

6xHis and myc

Expression

Mammalian

Mutation

F4C: c.11T>G, p.Phe4Cys

Promoter

CMV

Available Since

Aug. 1, 2014

Availability

Academic Institutions and Nonprofits only
pFA6a-GFP (F64L, S65T, V163A)-TRP1

Plasmid

#53184

Purpose

chromosomal tagging with stable GFP

Depositor

John Pringle

Article

Ko et al Mol Biol Cell. 2007 Dec;18(12):5139-53.

Insert

TRP1 (TRP1 Budding Yeast)

Use

Yeast genomic targeting

Tags

GFP (F64L, S65T, V163A)

Available Since

May 27, 2014

Availability

Academic Institutions and Nonprofits only
pET15b-At 14-3-3 phi

Plasmid

#44939

Depositor

Robert Ferl

Article

Sehnke et al Plant Physiol. 2006 Feb;140(2):647-60. E

Insert

14-3-3 phi (GF14 PHI Mustard Weed)

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

May 14, 2013

Availability

Academic Institutions and Nonprofits only
pEF5/FRT-DEST EVC2dW-Flag

Plasmid

#41007

Depositor

Rajat Rohatgi

Article

Dorn et al Dev Cell. 2012 Sep 12. pii: S1534-5807(1

Insert

Ellis van Creveld syndrome 2 (EVC2 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

deleted amino acids 1177-1220

Available Since

Nov. 14, 2012

Availability

Academic Institutions and Nonprofits only
HL 3722 (=HL 716 + pHL 404)

Plasmid

#30084

Depositor

Han Lim

Article

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Insert

pLtetO-1:MicC, pLlacO-1:hfq, pLtetO-1:ompC::gfp

Use

Synthetic Biology

Tags

GFP

Expression

Bacterial

Available Since

Sept. 28, 2011

Availability

Academic Institutions and Nonprofits only