We narrowed to 8,596 results for: Set

1. pRDA_888

   Plasmid

   #201165

   Purpose

   Lentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' p65, HSF1 and NLS

   Depositor

   John Doench

   Article

   Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

   Insert

   Puromycin resistance
   

   Use

   CRISPR and Lentiviral

   Promoter

   EFS

   Available Since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMSCVpuroATT

   Plasmid

   #47539

   Purpose

   Retroviral Gateway destination vector constructed by inserting Reading Frame Cassette A into pMSCVpuro (Clontech) using the Gateway Vector Conversion System (Invitrogen)

   Depositor

   David Weinstock

   Article

   Shindoh et al PLoS One. 2012;7(11):e49201. doi: 10.137

   Type

   Empty backbone

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Sept. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pAC150-PBLHL-4xHS-EF1a-DEST

   Plasmid

   #48234

   Purpose

   PiggyBac expression gateway destination vector with Insulator-flanked EF1a promoter gateway cassette and floxed Hygro selection marker

   Depositor

   Rudolf Jaenisch

   Article

   Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

   Type

   Empty backbone

   Use

   Piggybac

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   May 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. Spacer Switch Plasmid

   Plasmid

   #215346

   Purpose

   Facilitates the change in spacer sequence to Neisseria gonorrhoeae encoding the Nla CRISPRi system.

   Depositor

   Hank Seifert

   Article

   Geslewitz et al mBio. 2023 Dec 21:e0302523. doi: 10.1128

   Insert

   OpaD spacer; Chloramphenicol Resistance Cassette
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   April 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSLS.402

   Plasmid

   #187202

   Purpose

   Expresses retron Eco1 RT

   Depositor

   Seth Shipman

   Article

   Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

   Insert

   Retron Eco1 Reverse Transcriptase
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   mphR

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSEED-mScarlet3:ALFA

   Plasmid

   #219641

   Purpose

   Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in mScarlet3:ALFA using the SEED/Harvest method

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   mScarlet:ALFA SEED Cassette
   

   Available Since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. p3E-FRT-KanR-FRT pA

   Plasmid

   #82601

   Purpose

   3' entry vector containing FRT-flanked kanamycin resistance cassette (KanR) with pA for FLP-induced kanamycin resistance, drug selection

   Depositor

   Kryn Stankunas

   Article

   Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

   Insert

   FRT-Kan-FRT
   

   Promoter

   None

   Available Since

   Dec. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pR26 iBFP Dest

   Plasmid

   #74284

   Purpose

   Gene targeting vector for the mouse Rosa26 locus, including a loxP flanked stop cassette and BFP, for gateway cloning

   Depositor

   Ralf Kuehn

   Article

   Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

   Insert

   Rosa26 5-homology region
   

   Available Since

   May 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJ151-HDR

   Plasmid

   #113631

   Purpose

   Contains two recombination homology arms and loxP-flanked cassette encoding puromycin resistance gene, Venus fluorescence marker, thymidine kinase suicide gene, for scarless chromosomal engineering.

   Depositor

   Alex Kentsis

   Inserts

   Left homology arm
   Puromycin resistance gene
   Venus fluorescence gene
   Thymidine kinase
   Right homology arm
   

   Use

   Cre/Lox; Homology recombination selection vector

   Expression

   Bacterial and Mammalian

   Promoter

   EF1a promoter, EF1a promoter (T2A cleaving sequen…

   Available Since

   Sept. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMarVF1

    Plasmid

    #99579

    Purpose

    Minitransposon delivery vector pMarVF1 (suicide vector) in V. fischeri ES114

    Depositor

    Mark Mandel

    Article

    Brooks JF et al Proc Natl Acad Sci U S A. 2014 Dec 2;111

    Insert

    V. fischeri mariner transposon (with erythromycin-resistance (ErmR) cassette)
    

    Use

    Suicide vector in v. fischeri es114

    Available Since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pICH47742:FCP:Cas9YFP

    Plasmid

    #85986

    Purpose

    Golden Gate Level 1 cassette encoding a Cas9-YFP fusion under the FCP promoter

    Depositor

    Thomas Mock

    Article

    Hopes et al Plant Methods. 2016 Nov 24;12:49. doi: 1

    Insert

    Cas9
    

    Use

    CRISPR; Golden gate assembly

    Tags

    SV40 NLS, YFP

    Promoter

    FCP

    Available Since

    Jan. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMA-SpCas9-g1

    Plasmid

    #80784

    Purpose

    BsaI-based cloning of SpCas9 gRNA guide sequence, position 1/11/21 in the array

    Depositor

    Yonglun Luo

    Article

    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

    Insert

    CRISPR gRNA expression cassette (for SpCas9)
    

    Use

    CRISPR

    Tags

    na

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJAT11

    Plasmid

    #204290

    Purpose

    For inserting a phiC31 attP site. HDR integrant is marked with removable ie1-DsRed cassette, which is expressed in abdomen and mouthparts.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Insert

    ie1-DsRed
    

    Use

    CRISPR

    Promoter

    ie1

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMA-SpCas9-g4

    Plasmid

    #80787

    Purpose

    BsaI-based cloning of SpCas9 gRNA guide sequence, position 4/14/24 in the array

    Depositor

    Yonglun Luo

    Article

    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

    Insert

    CRISPR gRNA expression cassette (for SpCas9)
    

    Use

    CRISPR

    Tags

    na

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSLS.400

    Plasmid

    #187201

    Purpose

    Expresses retron Eco4 RT

    Depositor

    Seth Shipman

    Article

    Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

    Insert

    Retron Eco4 Reverse Transcriptase
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    mphR

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. Nme2sgRNA_pLKO

    Plasmid

    #119926

    Purpose

    Nme2Cas9 U6-driven sgRNA cassette

    Depositor

    Erik Sontheimer

    Article

    Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

    Insert

    NmeCas9 sgRNA
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMA-SpCas9-g2

    Plasmid

    #80785

    Purpose

    BsaI-based cloning of SpCas9 gRNA guide sequence, position 2/12/22 in the array

    Depositor

    Yonglun Luo

    Article

    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

    Insert

    CRISPR gRNA expression cassette (for SpCas9)
    

    Use

    CRISPR

    Tags

    na

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLP2

    Plasmid

    #209989

    Purpose

    Entry vector to store Level 0 resistance cassette parts and E. coli shuttle part (containing no E. coli ori)

    Depositor

    Georg Fritz, Thorsten Mascher

    Article

    Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. MTK234_008

    Plasmid

    #123902

    Purpose

    Encodes the GFP dropout cassette for multi-TU destination vectors as a type 234 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    GFP dropout for multiTU destination vectors
    

    Expression

    Mammalian

    Available Since

    Feb. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. LentiGuide_CjCas9-Puro

    Plasmid

    #202564

    Purpose

    hU6-BsmBI-gRNA-Puro

    Depositor

    Gerald Schwank

    Article

    Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

    Insert

    CjCas9 sgRNA cassette
    

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Sept. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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