We narrowed to 11,647 results for: emb
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Plasmid#227147PurposeVector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with two copies of UGI and hA3A* variant and HMG-D domain was inserted between hA3A* and eIscBn driven by CMV promoterDepositorInsertbpNLS-hA3A*-linker-HMG-D-linker-nOgeuIscB-v3-nucleoplasmin NLS-2XUGI-bpNLS
MutationD96R/E84R/V159R/H339APromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-SLC25A46-P2A-Electra1 (pAH9)
Plasmid#227614PurposeOverexpression of SLC25A46 in mammalian cell linesDepositorAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-myr-mTagBFP2-Flag (JDW 1183)
Plasmid#224492PurposeGateway compatible middle entry clone containing a Myristoylated mTagBP2 with a c-terminal Flag tag (cell membrane BFP marker)DepositorInsertmyr-mTagBFP2-Flag
UseGateway CloningAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-3xNLS-(G2SG2)2-Cre (JDW 1149)
Plasmid#224514PurposeA Gateway compatible middle entry clone containing a 3x nuclear localization signal followed by a multiple cloning site and a flexible linker fused to CreDepositorInsert3xNLS-(G2SG2)2-Cre
UseCre/LoxAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Tras light chain
Plasmid#216294PurposeExpresses anti-HER2 Tras Light chain in mammalian cells. To be paired with Tras heavy chain to form the anti-HER2 Tras FabDepositorInsertTras light chain
UseAffinity Reagent/ AntibodyTagsSignal peptideExpressionMammalianPromoterT7 promoterAvailable sinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
plentiCRISPR-v2 Puro KIAA0141/DELE1_sg1
Plasmid#218524PurposesgRNA targeting human KIAA0141/DELE1DepositorInsertDELE1 (DELE1 Human)
UseCRISPR and LentiviralAvailable sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
LBR-FKBP-GFP
Plasmid#222413PurposeTo express LBR with C-terminal GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cellsDepositorAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-lyn10-hLOV-ssrA-P2A-LOVPLD-mCherry-sspB
Plasmid#219557PurposePM-targeted iLID-LOVPLDDepositorInsertlyn10-iLID-LOVPLD
TagsmCherryExpressionMammalianAvailable sinceMay 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-Ctdnep1_Cter
Plasmid#196522PurposeEntry vector for Gateway with Ctdnep1_CterDepositorInsertCtdnep1-Cter (CTDNEP1 Human)
ExpressionBacterialMutationNo transmembrane domainPromoterCMV promoter; T7 promoterAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
p4118
Plasmid#198151Purposebacterial expression of sseF::HaloTag fusionDepositorInsertPsseA::sscB sseF::L16::HaloTag::HA (sseF Salmonella enterica serovar Typhimurium)
TagsHaloTag-HAExpressionBacterialAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pfa6a mScarlet-FKBP::KANMX
Plasmid#185905PurposePlasmid for cloning mScarlet fused to FKBP into yeast.DepositorInsertmScarlet-FKBP
TagsmScarlet fused to FKBPExpressionYeastPromoterSP6Available sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T Doc2beta C2AB(125) WT
Plasmid#134690PurposeEncodes cytoplasmic domain of Doc2beta for bacterial expression and purificationDepositorAvailable sinceNov. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-CαN(E12,14K)-mPAGFP
Plasmid#168495PurposeMammalian expression of the E12,14K mutant of the N-terminal 47 residues of mouse PKA Catalytic subunit α C-terminally tagged by monomeric paGFP.DepositorInsertE12,14K mutant of the N-terminal 47 residues of mouse PKA Catalytic subunit α C-terminally tagged by monomeric paGFP
ExpressionMammalianAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-CαN (K29,30A)-mVenus
Plasmid#168485PurposeMammalian expression of the K29,30A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus.DepositorInsertthe K29,30A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus
ExpressionMammalianAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-SRGAP3FBAR-EGFP
Plasmid#168506PurposeExpression of F-BAR domain (aa 1-492) of SRGAP3 with a C-terminal EGFP fusionDepositorAvailable sinceApril 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 SV40-AcrIIA4 (GB3333)
Plasmid#160605PurposeSV40-AcrIIA4 codon optimized for N.benthamianaDepositorInsertAcrIIA4
UseSynthetic BiologyMutationBsaI and BsmBI sites removedAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
UBQ10:sXVE:ER-GFP1–10
Plasmid#125664PurposeBinary vector for the inducible expression of ER-targeted GFP1–10 fragment that can be used to detect a protein tagged with GFP11DepositorInsertER-GFP1–10
UseSynthetic BiologyExpressionPlantPromoterUBQ10:sXVE:Available sinceAug. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
px335-sgRNA-EF(Cas9wt)-Rif1
Plasmid#122306PurposeExpresses sgRNA targeting mouse Rif1 and Cas9 in mammalian cellsDepositorInsertsgRNA for mouse Rif1 (Rif1 Synthetic)
ExpressionMammalianAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIRES:mTREK-1(K271Q)
Plasmid#133273PurposeMammalian expression vector. It will generate the mouse TREK-1 channel full lenght K271Q mutant fused to a N-terminal HA tagDepositorInsertPotassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 Mouse)
TagsHAExpressionMammalian and YeastMutationK271QAvailable sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTagBFP2-C1-AKAP1N31-FKBP-PI-PLCN187
Plasmid#139309PurposeN-terminal half of split PI-PLC targeted to mitochondriaDepositorInsertMus musculus Akap1(1-31) M16L: PTRSANS[GA]4ILSRM:TagBFP2: FKBP1A(3-108): [GGSA]4GG:L. monocytogenes PI-PLC(1-187)
ExpressionMammalianAvailable sinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only