We narrowed to 17,703 results for: puromycin
-
Plasmid#127374PurposeAllows for doxycycline-inducible BMP7 expression from the murine ROSA26 safe harbor locus upon CRISPR/Cas9-mediated genomic insertion and stable selection.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBMP7 (Bmp7 Mouse)
UseCRISPR and Mouse TargetingAvailable sinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-TurboID-V5-BRSK2_IDG-K
Plasmid#135272PurposeGateway destination clone of BRSK2 (human) tagged with N-terminal TurboID-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertTurboID-V5-BRSK2 (BRSK2 Human)
UseLentiviral; Gateway destinationTagsV5-TurboIDExpressionMammalianPromoterUbiquitinAvailable sinceJan. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
BII-ChPtW-smURFP
Plasmid#133399PurposePiggybac vector for constitutive expression of smURFP-NLSDepositorInsertsmURFP-NLS
ExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available sinceJan. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
p663-UBC-V5-BRSK1_IDG-K
Plasmid#135270PurposeGateway destination clone of BRSK1 (human) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomicsDepositorInsertV5-BRSK1 (BRSK1 Human)
UseLentiviral; Gateway destinationTagsV5ExpressionMammalianPromoterUbiquitinAvailable sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
BII-gR-PnTW
Plasmid#133356PurposePiggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications) coexpressed with tdTomato-NLSDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserttdTomato-NLS
TagsHA-tagged tdTomatoExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC57-PB-PGK-tetR-3xFLAG-HA-2A-Puro
Plasmid#129537PurposePiggyBac transposon carrying tetR-3xFLAG-HA-2A-Puro under the PGK promoter.DepositorInserttetR-3xFlag-HA-2A-Puro
UseSynthetic BiologyPromoterhPGK promoterAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro-U6-SpyCas9-TLR-MCV1-sgRNA1
Plasmid#117405PurposeSpyCas9 sgRNA 1 targeting TLR2.0DepositorInsertSpyCas9 sgRNA targeting TLR 2.0
UseLentiviralExpressionMammalianPromoterU6Available sinceDec. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC-STITCH
Plasmid#129535PurposeSTITCH (Synthetic Toplogical Insulator with TetO for CHromatin-engineering) with homology arms for MYC locus insertion.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSTITCH
UseCRISPR; Donor templateAvailable sinceDec. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
GWF180
Plasmid#133607PurposeCAG-DNCR2-VPR-IRES-Puro-WPRE-SV40PA-PGK-NS3aH1-tagBFP-SpdCas9DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertDNCR2-VPR, NS3aH1-tagBFP-SpdCas9
ExpressionMammalianAvailable sinceOct. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRv2-TRAF6-targeting sgRNA 2
Plasmid#131346PurposegRNA targeting mouse TRAF6DepositorAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAPM-D4 miR30-TRIM28 ts1
Plasmid#115862PurposeTRIM28 knockdownDepositorAvailable sinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Tsc2-g2)-PGKpuroBFP-W
Plasmid#105040PurposeLentiviral gRNA plasmid targeting mouse Tsc2 , co-expression of TagBFPDepositorAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL3-sgACSL3.C-Cas9-P2A-Puro
Plasmid#129412PurposeEncoding Cas9 and sgACSL3.C for CRISPR/Cas9 mediated HDR tagging of endogenous human ACSL3 C-terminusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSpCas9 and sgACSL3.C (ACSL3 Human)
UseCRISPRTagsP2A-PuroExpressionMammalianPromoterhU6Available sinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLC-CUL4A WT-P2A-Puro
Plasmid#124304PurposeLentiviral vector for expression of wild-type CUL4A-P2A-Puro casette from a CMV promoterDepositorAvailable sinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
library backbone 3'LTR gRNA and barcode
Plasmid#127397PurposeDigestion by BstXI and BamHI to release the backboneDepositorTypeEmpty backboneUseLentiviralTagsPuro-mCherryAvailable sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRSITEP-puro-shWRN1-C911
Plasmid#125784Purposea seed-matched control for pRSITEP-puro-shWRN1DepositorInsertshWRN1-C911 (WRN Human)
UseRNAiAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLKO.1-puro-shWRN2
Plasmid#125782Purposeconstitutive expression of a short-hairpin RNA targeting human WRNDepositorInsertshWRN2 (WRN Human)
UseRNAiAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVX-M-Flag-BAP1-G185R
Plasmid#125849Purposeexpress mutant BAP1-G185RDepositorAvailable sinceJune 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSLQ1371-sgSTAT3-1
Plasmid#121425PurposesgSTAT3-1 sequence: GTCAGGATAGAGATAGACCAG. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.DepositorInsertsgSTAT3-1
UseCRISPR and LentiviralTagsmCherryExpressionMammalianPromotermouse U6Available sinceApril 22, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Myog'-2A-mCherryNLS-PGK-Puro
Plasmid#69548PurposeDonor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsUseMouse TargetingTags2x nuclear localization signal and PVT1-2A "…MutationSilent V205V mutation in myogenin gene to prevent…Available sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only