We narrowed to 5,366 results for: pcDNA3.1+

Showing: 931 - 960 of 5366 results

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pcDNA3.1his p66shc S36A

Plasmid

#10973

Depositor

Toren Finkel

Article

Nemoto et al Science. 2002 Mar 29. 295(5564):2450-2.

Insert

p66shc (SHC1 Human)

Tags

His

Expression

Mammalian

Mutation

S36A

Available Since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-Firefly-ccdB

Plasmid

#87069

Purpose

Mammalian Gateway-compatible expression vector backbone with an N-terminal Firefly luciferase

Depositor

Mikko Taipale

Type

Empty backbone

Tags

Firefly luciferase

Expression

Mammalian

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-FluoSTEP-JNKAR

Plasmid

#181857

Purpose

Genetically encoded FRET-based sensor for monitoring JNK kinase activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

Depositor

Jin Zhang

Article

Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

Insert

FluoSTEP-JNKAR

Tags

mRuby2 and sfGFP(1-10)

Expression

Mammalian

Promoter

CMV

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-FluoSTEP-AktAR

Plasmid

#181856

Purpose

Genetically encoded FRET-based sensor for monitoring Akt activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

Depositor

Jin Zhang

Article

Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

Insert

FluoSTEP-AktAR

Tags

mRuby2 and sfGFP(1-10)

Expression

Mammalian

Promoter

CMV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CFP-KRAS4B

Plasmid

#112717

Purpose

Expresses CFP-KRAS4B fusion protein used for FRET studies in mammalian cells.

Depositor

Kenneth Westover

Article

Ambrogio et al Cell. 2018 Feb 8;172(4):857-868.e15. doi

Insert

CFP-KRAS4B (KRAS Human)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FLIPglu-3.2mDelta13V

Plasmid

#18011

Depositor

Wolf Frommer

Article

Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

Insert

FLIPglu F16A S112A Delta13

Tags

ECFP and Venus

Expression

Mammalian

Mutation

F16A, S112A in binding protein

Available Since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-dCas9-CibN

Plasmid

#60552

Purpose

Expresses dCas9-CibN in mammalian cells

Depositor

Charles Gersbach

Article

Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

Insert

dCas9-CibN

Use

CRISPR

Tags

FLAG and SV40 NLS

Expression

Mammalian

Mutation

inactivating Cas9 mutations D10A and H840A

Promoter

CMV

Available Since

Jan. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-Golgi-RasAR

Plasmid

#205570

Purpose

Golgi membrane-targeted RasAR.

Depositor

Jin Zhang

Article

Weeks et al J Am Chem Soc. 2022 Sep 28;144(38):17432

Insert

Golgi-RasAR (RAF1, GOLGB1 Human, Synthetic)

Tags

Golgin B1 (Giantin) residues 3131-3259

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pY012 (pcDNA3.1-hCMtCpf1)

Plasmid

#69984

Purpose

Expresses humanized CMtCpf1

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

hCMtCpf1

Use

CRISPR

Tags

NLS-3xHA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-sapphireAKAR-NLS

Plasmid

#118467

Purpose

cp-T-sapphire-based single-color PKA biosensor targeted to the nucleus.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

sapphireAKAR-NLS

Tags

Nuclear localization signal (NLS)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsMex3B_AD

Plasmid

#148478

Purpose

Mammalian Expression of HsMex3B

Depositor

Elisa Izaurralde

Insert

HsMex3B (MEX3B Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/HisC 14-3-3 epsilon wt

Plasmid

#11942

Depositor

Michael Yaffe

Article

Brunet et al J Cell Biol. 2002 Mar 4. 156(5):817-28.

Insert

Ywhae (Ywhae Rat)

Tags

His

Expression

Mammalian

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-hFluc-V5-6xbsMS2_U

Plasmid

#147639

Purpose

Mammalian Expression of 6xbsMS2

Depositor

Elisa Izaurralde

Insert

6xbsMS2

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 FLIPE-100u

Plasmid

#13543

Depositor

Wolf Frommer

Article

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Insert

FLIP-E S118W

Tags

ECFP and Venus

Expression

Mammalian

Mutation

S118W in binding protein

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pF153 pcDNA3.1(+)SOD1G37R

Plasmid

#26399

Depositor

Elizabeth Fisher

Article

Stevens et al PLoS One. 2010 . 5(3):e9541.

Insert

SOD1 (SOD1 Human)

Expression

Mammalian

Mutation

G37R

Available Since

Nov. 23, 2010

Availability

Academic Institutions and Nonprofits only
pY017 (pcDNA3.1-hPcCpf1)

Plasmid

#69989

Purpose

Expresses humanized PcCpf1

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

hPcCpf1

Use

CRISPR

Tags

NLS & 3xHA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 (-) hYAP 1-1delta

Plasmid

#124142

Purpose

Protein expression for funtional studies of cell growth promotion

Depositor

Megan Finch-Edmondson, Marius Sudol

Article

Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

Insert

YAP1 (YAP1 Human)

Use

Unspecified

Mutation

None (Wild Type)

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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mcherry Gamma 9 ---pcDNA3.1

Plasmid

#64203

Purpose

G protein Gamma cone(9) subunit tagged with mcherry on the N terminus

Depositor

Narasimhan Gautam

Article

Karunarathne et al Proc Natl Acad Sci U S A. 2013 Apr 23;11

Insert

G protein Gamma 9 (GNGT2 Human)

Tags

6xHis and mcherry

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-MS2-HA-HsMex3C_AD

Plasmid

#148481

Purpose

Mammalian Expression of HsMex3C

Depositor

Elisa Izaurralde

Insert

HsMex3C (MEX3C Human)

Expression

Mammalian

Mutation

L11P in Mex3C

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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FLAG-HA-BLMP-1-pcDNA3.1-

Plasmid

#52512

Purpose

expresses C. elegans BLMP-1 in mammalian cells with FLAG-HA tag at N-terminus

Depositor

Adam Antebi

Article

Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

Insert

Blmp-1 (blmp-1 Nematode)

Tags

FLAG-HA

Expression

Mammalian

Mutation

N75S mutation compared GenBank reference sequence…

Promoter

CMV

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Renilla-ccdB

Plasmid

#87068

Purpose

Mammalian Gateway-compatible expression vector backbone with an N-terminal Renilla luciferase

Depositor

Mikko Taipale

Type

Empty backbone

Tags

Renilla luciferase

Expression

Mammalian

Available Since

March 6, 2017

Availability

Academic Institutions and Nonprofits only

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Rat NCX1.7 (pcDNA3.1+)

Plasmid

#75192

Purpose

Mammalian expression of SLC8A1

Depositor

Jonathan Lytton

Article

Lee et al J Biol Chem. 1994 May 27;269(21):14849-5

Insert

SLC8A1

Expression

Mammalian

Promoter

CMV

Available Since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pY005 (pcDNA3.1-hLb3Cpf1)

Plasmid

#69977

Purpose

Expresses humanized Lb3Cpf1

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

hLb3Cpf1

Use

CRISPR

Tags

NLS & 3xHA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+)-RABIF-FLAG

Plasmid

#135558

Purpose

Express human RABIF in mammalian cells, FLAG at N terminal

Depositor

Jingshi Shen

Article

Gulbranson et al Proc Natl Acad Sci U S A. 2017 Sep 26;11

Insert

RABIF (RABIF Human)

Tags

Flag

Expression

Insect

Promoter

T7 promoter

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsUNR_AA

Plasmid

#148239

Purpose

Mammalian Expression of HsUNR

Depositor

Elisa Izaurralde

Insert

HsUNR

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(−) mCER-Prx2

Plasmid

#158224

Purpose

Genetically encoded HomoFRET-based probe to monitor peroxiredoxin2 (Prx2) oligomerization (for transient transfection in mammalian cells)

Depositor

Tobias Dick

Article

Pastor-Flores et al Proc Natl Acad Sci U S A. 2020 Jun 29. p

Insert

mCER-Prx2

Expression

Mammalian

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-aDTX-lumitoxin

Plasmid

#51686

Purpose

Expression vector for aDTX-lumitoxin

Depositor

Edward Boyden

Article

Schmidt et al Nat Commun. 2014 Jan 10;5:3019. doi: 10.

Insert

aDTX-lumitoxin

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 myc-PITPalpha

Plasmid

#58277

Purpose

mammalian expression of human PITP alpha

Depositor

Shamshad Cockcroft

Insert

PITP alpha (PITPNA Human)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 PA-mCit-BAD

Plasmid

#113459

Purpose

ProteinA-mCitrine-BAD expression vector (positive control together with pcDNA3.1 NL-BCL2L1)

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Insert

BCL2 associated agonist of cell death (BAD Human)

Tags

ProteinA-mCitrine

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_mGFP-FGFR3-K650E

Plasmid

#191757

Purpose

Expression of N-terminally mGFP labelled mutated FGFR3 (isoform IIIc)-K650E mutation (associated with TD2; target mutation: c.1948A>G; silent mutation added on purpose: c.1938C>T)

Depositor

Irene Tiemann-Boege

Article

Hartl et al J Biol Chem. 2023 Feb;299(2):102832. doi

Insert

mGFP-FGFR3IIIc

Tags

mGFP

Expression

Mammalian

Mutation

c.1948A>G (p.K650E) + silent mutation c.1938C&…

Available Since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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