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Showing: 1551 - 1600 of 12534 results

  1. DAPK3_HUMAN_D0

    Plasmid
    #79721
    Purpose
    This plasmid encodes the kinase domain of DAPK3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
    Depositor
    John Chodera, Markus Seeliger, Nicholas Levinson
    Article
    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/
    Insert
    DAPK3 (DAPK3 Human)
    Use
    Tags
    His10-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  5. MAPK3_HUMAN_D0

    Plasmid
    #79702
    Purpose
    This plasmid encodes the kinase domain of MAPK3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
    Depositor
    John Chodera, Markus Seeliger, Nicholas Levinson
    Article
    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/
    Insert
    MAPK3 (MAPK3 Human)
    Use
    Tags
    His10-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  6. pGFP-HsSyx3

    Plasmid
    #179289
    Purpose
    E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 393-792) with N-terminal His6 + GFP + TEV protease cleavage site
    Depositor
    Debra Hansen
    Article
    Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.
    Insert
    Syx
    Use
    Tags
    His6 + GFP + TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    residues 393-792 from accession number NP_0010361…
    Promoter
    T7
    Available since
    June 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    179289-plasmid-map-sequence-id-354922

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  7. pHsSyx3-MBP

    Plasmid
    #179286
    Purpose
    E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 393-792) with C-terminal TEV protease cleavage site + mature maltose binding protein + His6
    Depositor
    Debra Hansen
    Article
    Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.
    Insert
    Syx
    Use
    Tags
    TEV protease cleavage site + mature maltose bindi…
    Expression
    Bacterial
    Mutation
    residues 393-792 from accession number NP_0010361…
    Promoter
    T7
    Available since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    179286-plasmid-map-sequence-id-354920

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  29. E2AK2_HUMAN_D0

    Plasmid
    #79712
    Purpose
    This plasmid encodes the kinase domain of E2AK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
    Depositor
    John Chodera, Markus Seeliger, Nicholas Levinson
    Article
    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/
    Insert
    E2AK2 (EIF2AK2 Human)
    Use
    Tags
    His10-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  32. KC1G1_HUMAN_D0

    Plasmid
    #79691
    Purpose
    This plasmid encodes the kinase domain of KC1G1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
    Depositor
    John Chodera, Markus Seeliger, Nicholas Levinson
    Article
    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/
    Insert
    KC1G1 (CSNK1G1 Human)
    Use
    Tags
    His10-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  33. CSK22_HUMAN_D0

    Plasmid
    #79704
    Purpose
    This plasmid encodes the kinase domain of CSK22. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.
    Depositor
    John Chodera, Markus Seeliger, Nicholas Levinson
    Article
    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/
    Insert
    CSK22 (CSNK2A2 Human)
    Use
    Tags
    His10-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Nov. 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  34. T7-VEE-OKS-iG

    Plasmid
    #58974
    Purpose
    RNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2 separated by 2A peptides followed by an IRES then GLIS1 and a second IRES and PuroR ORF
    Depositor
    Steven Dowdy
    Article
    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.
    Inserts
    Oct4 (POU5F1 Human)
    Klf4 (KLF4 Human)
    Sox2 (SOX2 Human)
    GLIS (GLIS1 Human)
    Use
    Venezuelan equine encephalitis (vee) virus rna re…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    58974-plasmid-map-sequence-id-240741

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  35. SP6-VEE-OKS-iM

    Plasmid
    #58973
    Purpose
    RNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2, separated by 2A peptides followed by an IRES then c-myc and a second IRES and PuroR ORF
    Depositor
    Steven Dowdy
    Article
    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.
    Inserts
    Oct4 (POU5F1 Human)
    Klf4 (KLF4 Human)
    Sox2 (SOX2 Human)
    cMyc (MYC Human)
    Use
    Venezuelan equine encephalitis (vee) virus rna re…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    58973-plasmid-map-sequence-id-91416

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  36. SP6-VEE-OKS-iG

    Plasmid
    #58975
    Purpose
    RNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2 separated by 2A peptides followed by an IRES then GLIS1 and a second IRES and PuroR ORF
    Depositor
    Steven Dowdy
    Article
    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.
    Inserts
    Oct4 (POU5F1 Human)
    Klf4 (KLF4 Human)
    Sox2 (SOX2 Human)
    GLIS (GLIS1 Human)
    Use
    Venezuelan equine encephalitis (vee) virus rna re…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    58975-plasmid-map-sequence-id-91419

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  37. Dynein-2_complex

    Plasmid
    #132536
    Purpose
    Full length human (Hs) IFT dynein (dynein-2) complex for Sf9 expression. Contains His-ZZ-TEV-SNAPf-HC, WDR60, WDR34-Strep, LIC3, TCTEX-1, TCTEX1D2, LC8-1, LC8-2, TCTEX-3, Rbl-1, Rbl-2, LC8-like.
    Depositor
    Andrew Carter
    Article
    Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1
    Inserts
    DYNC2H1 (DYNC2H1 Human)
    DYNC2LI1 (DYNC2LI1 Human)
    WDR34 (WDR34 Human)
    WDR60 (WDR60 Human)
    DYNLL1 (DYNLL1 Human)
    DYNLL2 (DYNLL2 Human)
    DYNLRB1 (DYNLRB1 )
    DYNLRB2 (DYNLRB2 Human)
    DYNLT1 (DYNLT1 Human)
    TCTEX1D2 (TCTEX1D2 Human)
    DYNLT3 (DYNLT3 Human)
    LC8-like (LOC392067 Human)
    Use
    Tags
    8x His, Linker-TEV-linker-TEV, SNAPf, Strep, and …
    Expression
    Insect
    Mutation
    Codon optimised for Sf9 expression
    Promoter
    Available since
    Feb. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    132536-plasmid-map-sequence-id-270901

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  38. pHS12-mCherry

    Plasmid
    #25444
    Depositor
    Benjamin Glick
    Insert
    mCherry
    Use
    Tags
    COX4 Presequence
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 24, 2010
    Availability
    Academic Institutions and Nonprofits only
    25444-plasmid-map-sequence-id-43419

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  39. pmECFP-1

    Plasmid
    #38772
    Depositor
    Benjamin Glick
    Type
    Empty backbone
    Use
    Tags
    mECFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 15, 2012
    Availability
    Academic Institutions and Nonprofits only
    38772-plasmid-map-sequence-id-48799

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  40. pmEYFP-1

    Plasmid
    #38773
    Depositor
    Benjamin Glick
    Type
    Empty backbone
    Use
    Tags
    mEYFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 15, 2012
    Availability
    Academic Institutions and Nonprofits only
    38773-plasmid-map-sequence-id-48800

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  41. T7-VEE-OKS-iM

    Plasmid
    #58972
    Purpose
    RNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2, separated by 2A peptides followed by an IRES then c-myc and a second IRES and PuroR ORF
    Depositor
    Steven Dowdy
    Article
    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.
    Inserts
    Oct4 (POU5F1 Human)
    Klf4 (KLF4 Human)
    Sox2 (SOX2 Human)
    cMyc (MYC Human)
    Use
    Venezuelan equine encephalitis (vee) virus rna re…
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    26S subgenomic promoter and IRES
    Available since
    Oct. 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    58972-plasmid-map-sequence-id-466890

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  43. pDG03769

    Plasmid
    #195996
    Purpose
    Nanobody Proj-Nano28 production
    Depositor
    Dirk Görlich
    Insert
    H14-NEDD8- ProjNano28-Cys
    Use
    E.coli expression
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    195996-plasmid-map-sequence-id-385940

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  44. pDG04135

    Plasmid
    #196013
    Purpose
    Nanobody Zasp-Nano65 production
    Depositor
    Dirk Görlich
    Insert
    H14-SUMO-Cys- ZaspNano65-Cys
    Use
    E.coli expression
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196013-plasmid-map-sequence-id-385939

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  45. pDG03781

    Plasmid
    #195995
    Purpose
    Nanobody Sls-Nano48 production
    Depositor
    Dirk Görlich
    Insert
    H14-NEDD8- SlsNano48-Cys
    Use
    E.coli expression
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    195995-plasmid-map-sequence-id-385938

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  46. pDG04136

    Plasmid
    #196014
    Purpose
    Nanobody Zasp-Nano66 production
    Depositor
    Dirk Görlich
    Insert
    H14-SUMO-Cys- ZaspNano66-Cys
    Use
    E.coli expression
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196014-plasmid-map-sequence-id-385937

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  47. pDG03772

    Plasmid
    #196000
    Purpose
    Nanobody Proj-Nano33 production
    Depositor
    Dirk Görlich
    Insert
    H14-NEDD8- ProjNano33-Cys
    Use
    E.coli expression
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196000-plasmid-map-sequence-id-385935

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  48. pDG04092

    Plasmid
    #196009
    Purpose
    Nanobody Obs-Nano59 production
    Depositor
    Dirk Görlich
    Insert
    H14-SUMO-Cys- ObsNano59-Cys
    Use
    E.coli expression
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196009-plasmid-map-sequence-id-385930

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  49. pDG03770

    Plasmid
    #195998
    Purpose
    Nanobody Proj-Nano29 production
    Depositor
    Dirk Görlich
    Insert
    H14-NEDD8- ProjNano29-Cys
    Use
    E.coli expression
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    195998-plasmid-map-sequence-id-385926

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  50. pDG04091

    Plasmid
    #196008
    Purpose
    Nanobody Obs-Nano58 production
    Depositor
    Dirk Görlich
    Insert
    H14-SUMO-Cys- ObsNano58-Cys
    Use
    E.coli expression
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196008-plasmid-map-sequence-id-385923

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Showing: 1551 - 1600 of 12534 results