We narrowed to 75,668 results for: Rest

1. pAAV-U6-sgRNA(OXTR.1)-CMV-eGFP

   Plasmid

   #194015

   Purpose

   Expresses a gRNA that targets OXTR coding sequence in multiple (rodent) species and eGFP

   Depositor

   Larry Young

   Article

   Boender et al Sci Adv. 2023 Jun 2;9(22):eadf4950. doi:

   Inserts

   sgRNA(Oxtr.1)
   eGFP
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and u6

   Available since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pTL

   Plasmid

   #203176

   Purpose

   Centromeric yeast expression vector, leucine selection

   Depositor

   William Sessa

   Article

   Galosi et al Brain. 2022 Mar 29;145(1):208-223. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-U6-sgRNA(OXTR.2)-CMV-eGFP

   Plasmid

   #194016

   Purpose

   Expresses a gRNA that targets OXTR coding sequence in multiple (rodent) species and eGFP

   Depositor

   Larry Young

   Article

   Boender et al Sci Adv. 2023 Jun 2;9(22):eadf4950. doi:

   Inserts

   sgRNA(Oxtr.2)
   eGFP
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   cmb and u6

   Available since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3-FLAG-RPS14(89-151)

   Plasmid

   #122299

   Purpose

   expresses a part of RPS14 protein with a flag tag

   Depositor

   Gerardo Ferbeyre

   Article

   Lessard et al Nat Cell Biol. 2018 Jul;20(7):789-799. d

   Insert

   RPS14 (RPS14 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   deleted amino acids 1 to 88

   Promoter

   CMV

   Available since

   March 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3-FLAG-RPS14(2-88)

   Plasmid

   #122298

   Purpose

   expresses a part of RPS14 protein with a flag tag

   Depositor

   Gerardo Ferbeyre

   Article

   Lessard et al Nat Cell Biol. 2018 Jul;20(7):789-799. d

   Insert

   RPS14 (partial) (RPS14 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   deleted amino acids 89 to 151

   Promoter

   CMV

   Available since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGP-AAV-syn-FLEX-Volton2-ST-WPRE

   Plasmid

   #172907

   Purpose

   AAV-mediated expression of voltage sensor under the Syn promoter, Cre-dependent expression; soma localization targeting sequence

   Depositor

   GENIE Project

   Article

   Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

   Has Service

   AAV1

   Insert

   Voltron2-ST
   

   Use

   AAV and Cre/Lox

   Tags

   soma localization targeting sequence

   Expression

   Mammalian

   Mutation

   A122D

   Promoter

   hSynapsin1

   Available since

   Feb. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGP-AAV-syn-FLEX-Ace2N-4AA-mNeon-ST A122D WPRE

   Plasmid

   #172908

   Purpose

   AAV-mediated expression of voltage sensor under the Syn promoter, Cre-dependent expression; soma localization targeting sequence

   Depositor

   GENIE Project

   Article

   Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

   Has Service

   AAV1

   Insert

   Ace2N-4AA-mNeon-ST A122D
   

   Use

   AAV and Cre/Lox

   Tags

   soma localization targeting sequence

   Expression

   Mammalian

   Mutation

   A122D

   Promoter

   hSynapsin1

   Available since

   Nov. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. AV0hSynWS

   Plasmid

   #124966

   Purpose

   Express gene of interest in neurons

   Depositor

   Kazuhiro Oka

   Type

   Empty backbone

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHIV-dTomato

   Plasmid

   #21374

   Purpose

   self-inactivating lentiviral plasmid for co-expression of your gene of interest and dTomato

   Depositor

   Bryan Welm

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 19, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCDH-EF1

    Plasmid

    #72266

    Purpose

    Express gene of interest under EF-1 promoter

    Depositor

    Kazuhiro Oka

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 11, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. pJPN0018

    Plasmid

    #112083

    Purpose

    eCFP module CT

    Depositor

    Christian Chevalier

    Insert

    eCFP
    

    Use

    Tags

    Expression

    Plant

    Mutation

    BsaI and BpiI restriction sites have been remove…

    Promoter

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJPN0016

    Plasmid

    #112077

    Purpose

    tagRFP module CT

    Depositor

    Christian Chevalier

    Insert

    TagRFP
    

    Use

    Tags

    Expression

    Plant

    Mutation

    BsaI and BpiI restriction sites have been remove…

    Promoter

    Available since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pUltra-hot

    Plasmid

    #24130

    Purpose

    3rd generation Lentiviral vector for bi-cistronic expression of mCherry and the gene of interest

    Depositor

    Malcolm Moore

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 26, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCDH-CB

    Plasmid

    #72267

    Purpose

    Express gene of interest under CB promoter

    Depositor

    Kazuhiro Oka

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    chicken beta-actin + CMV enhancer

    Available since

    Jan. 11, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pJPN0003

    Plasmid

    #112080

    Purpose

    GFP module NT2

    Depositor

    Christian Chevalier

    Insert

    GFP
    

    Use

    Tags

    Expression

    Plant

    Mutation

    BsaI and BpiI restriction sites have been remove…

    Promoter

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJPN0015

    Plasmid

    #112081

    Purpose

    tagRFP module NT2

    Depositor

    Christian Chevalier

    Insert

    TagRFP
    

    Use

    Tags

    Expression

    Plant

    Mutation

    BsaI and BpiI restriction sites have been remove…

    Promoter

    Available since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCDH-PGK

    Plasmid

    #72268

    Purpose

    Express gene of interest under PGK (phosphoglycerate kinase I) promoter

    Depositor

    Kazuhiro Oka

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Jan. 11, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pORFE0011

    Plasmid

    #112075

    Purpose

    pcUBI promoter module Pro+5U

    Depositor

    Christian Chevalier

    Insert

    pcUBI promoter
    

    Use

    Tags

    Expression

    Plant

    Mutation

    BsaI and BpiI restriction sites have been remove…

    Promoter

    Available since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AV0-hSyn-SV40pA

    Plasmid

    #124965

    Purpose

    Express gene of interest under human Syn promoter

    Depositor

    Kazuhiro Oka

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AV0-BWS-2.0

    Plasmid

    #124976

    Purpose

    Express gene of interest under EF1 promoter

    Depositor

    Kazuhiro Oka

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. TY5_hisAI_2micron

    Plasmid

    #138622

    Purpose

    Ty5 restrotransposon with a reversed artificial intron used for Calling Cards experiment

    Depositor

    Rob Mitra

    Insert

    TY5
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Gal1_10

    Available since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pJPN0017

    Plasmid

    #112082

    Purpose

    eCFP module CDS1ns

    Depositor

    Christian Chevalier

    Insert

    eCFP without stop codon
    

    Use

    Tags

    Expression

    Plant

    Mutation

    BsaI and BpiI restriction sites have been remove…

    Promoter

    Available since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pUltra-Smurf

    Plasmid

    #48974

    Purpose

    3rd generation Lentiviral vector with bi-cistronic expression of the blue fluorescent protein AmCyan and the gene of interest.

    Depositor

    Malcolm Moore

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    AmCyan

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pRS416-Syf1

    Plasmid

    #234113

    Purpose

    Syf1 region PCR, restriction digest and ligation into pRS416 backbone

    Depositor

    Aaron Hoskins

    Insert

    Syf1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pRS414-Syf1

    Plasmid

    #234114

    Purpose

    Syf1 region PCR, restriction digest and ligation into pRS414 backbone

    Depositor

    Aaron Hoskins

    Insert

    Syf1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pOpen-EcoRV

    Plasmid

    #165548

    Purpose

    Type II restriction enzyme that recognizes the double-stranded sequence GATATC and cleaves after T-1.

    Depositor

    Jennifer Molloy, FreeGenes Project, Drew Endy

    Insert

    EcoRV
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 10, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. pOpen-SapIR

    Plasmid

    #165537

    Purpose

    Type II restriction enzyme that recognizes the double-stranded sequence GCTCTTC(N) and cleaves after N-1.

    Depositor

    Jennifer Molloy, FreeGenes Project, Drew Endy

    Insert

    SapI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 5, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. pOpen-EcoRIR

    Plasmid

    #165504

    Purpose

    Type II restriction enzyme that recognizes the double-stranded sequence GAATTC and cleaves after G-1.

    Depositor

    Jennifer Molloy, FreeGenes Project, Drew Endy

    Insert

    EcoRI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. pOpen-XbaIR

    Plasmid

    #165567

    Purpose

    Type II restriction enzyme that recognizes the double-stranded sequence TCTAGA and cleaves after T-1.

    Depositor

    Jennifer Molloy, FreeGenes Project, Drew Endy

    Insert

    XbaI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 18, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. pOpen-PstIR

    Plasmid

    #165526

    Purpose

    Restriction enzyme that recognizes the double-stranded sequence CTGCAG and cleaves after A-5.

    Depositor

    Jennifer Molloy, FreeGenes Project, Drew Endy

    Insert

    PstI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 5, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

31. pCS2+mScarlet3-C Cloning Vector

    Plasmid

    #220616

    Purpose

    To express a protein of interest fused to the C-terminus of mScarlet3 (unpublished)

    Depositor

    Ken-Ichi Takemaru

    Type

    Empty backbone

    Use

    Tags

    mScarlet3 fusion

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pOpen-HindIIIR

    Plasmid

    #165508

    Purpose

    Type II restriction enzyme that recognizes the double-stranded sequence AAGCTT and cleaves after A-1.

    Depositor

    Jennifer Molloy, FreeGenes Project, Drew Endy

    Insert

    HindIII
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

33. pOpen-NcoIR

    Plasmid

    #165568

    Purpose

    Type II restriction enzyme that recognizes the double-stranded sequence CCATGG and cleaves after C-1.

    Depositor

    Jennifer Molloy, FreeGenes Project, Drew Endy

    Insert

    NcoI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 18, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

34. pRS414-Fyv6-CFLAG

    Plasmid

    #234133

    Purpose

    Restriction digest from pUC18-CFLAG-Fyv6 and ligation into pRS414

    Depositor

    Aaron Hoskins

    Insert

    Fyv6
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pRS414-NFLAG-Fyv6

    Plasmid

    #234139

    Purpose

    Restriction digest from pUC18-NFLAG-Fyv6 and ligation into pRS414

    Depositor

    Aaron Hoskins

    Insert

    Fyv6
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pUltra-Plus

    Plasmid

    #161775

    Purpose

    3rd generation lentiviral multicistronic vector for mammalian expression of three genes of interest

    Depositor

    Jorge Luis Arias-Arias

    Type

    Empty backbone

    Use

    Lentiviral; Multicistronic

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UbC

    Available since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pOpen-Eco31I

    Plasmid

    #165506

    Purpose

    Type II restriction enzyme that recognized the double-stranded sequence. GGTCTC (N) and cleaves after N-1; isoschizomer of BsaI.

    Depositor

    Jennifer Molloy, FreeGenes Project, Drew Endy

    Insert

    Eco31I
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

38. pUC-L3L2

    Plasmid

    #114019

    Purpose

    Create Entry clones for Gateway LR reactions through simple restriction enzyme based cloning

    Depositor

    Christopher Grefen

    Insert

    ccdB gene, Chloramphenicol resistance gene
    

    Use

    Gateway

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pUC-L1L4

    Plasmid

    #114018

    Purpose

    Create Entry clones for Gateway LR reactions through simple restriction enzyme based cloning

    Depositor

    Christopher Grefen

    Insert

    ccdB gene, Chloramphenicol resistance gene
    

    Use

    Gateway

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pUC-L1L2

    Plasmid

    #114017

    Purpose

    Create Entry clones for Gateway LR reactions through simple restriction enzyme based cloning

    Depositor

    Christopher Grefen

    Insert

    ccdB gene, Chloramphenicol resistance gene
    

    Use

    Gateway

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pOpen-NotIR (GC)

    Plasmid

    #165507

    Purpose

    Type II restriction enzyme that recognizes the double-stranded sequence GCGGCCGC and cleaves after C-1.

    Depositor

    Jennifer Molloy, FreeGenes Project, Drew Endy

    Insert

    NotI (GC-rich recognition site)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

42. 371-A

    Plasmid

    #97026

    Purpose

    yORF

    Depositor

    Chris Jeans

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pLenti-sgRNA

    Plasmid

    #71409

    Purpose

    There is no gene/insert. It is a backbone plasmid that others can use to insert sgRNAs of interest. The cloning site for this BsmBI

    Depositor

    Eric Lander, David Sabatini

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. Flag-APEX3-C Cloning Vector

    Plasmid

    #220615

    Purpose

    To express a protein of interest fused to the C-terminus of Flag-APEX3 for proximity labeling or TEM (unpublished)

    Depositor

    Ken-Ichi Takemaru

    Type

    Empty backbone

    Use

    Tags

    Flag-APEX3 fusion

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pOpen-SfiIR (cDNA)

    Plasmid

    #165528

    Purpose

    Type II restriction enzyme that recognizes the double-stranded sequence GGCCNNNNNGGCC and cleaves after N-4.

    Depositor

    Jennifer Molloy, FreeGenes Project, Drew Endy

    Insert

    SfiI (used to create cDNA libraries)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 5, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

46. HA-MAO-N Mitochondrial Membrane-Targeting Cloning Vector

    Plasmid

    #139015

    Purpose

    To express a protein of interest fused to the N-terminus of HA-MAO for mitochondrial outer membrane targeting

    Depositor

    Ken-Ichi Takemaru

    Type

    Empty backbone

    Use

    Tags

    HA-MAO

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pSLfa[Tc'hsp5'3'UTR]fa

    Plasmid

    #86450

    Purpose

    Heat shock-inducible promoter including hsp-UTRs, ubiquitous expression upon heat-shock of any inserted gene of interest in Tribolium; (Shuttle plasmid)

    Depositor

    Gregor Bucher

    Insert

    Tc'hsp5'3'UTR
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. Flag-2xAPEX2-C Cloning Vector

    Plasmid

    #136991

    Purpose

    To express a protein of interest fused to the C-terminus of Flag-2xAPEX2 for proximity labeling or TEM

    Depositor

    Ken-Ichi Takemaru

    Type

    Empty backbone

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. 371-U

    Plasmid

    #97036

    Purpose

    MYFQSNA-yORF

    Depositor

    Chris Jeans

    Type

    Empty backbone

    Use

    Tags

    MYFQSNA

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pLKO.3G_X7

    Plasmid

    #171213

    Purpose

    3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses eGFP for selection. Please see cloning information for suggested restriction sites

    Depositor

    Jenny Wang

    Insert

    N/A; empty backbone
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge