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pMaCTag-P24

Plasmid

#120035

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with TAP

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

TAP

Use

Pcr template

Tags

TAP

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P17

Plasmid

#120028

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mEos4b

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mEos4b

Use

Pcr template

Tags

mEos4b

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P08

Plasmid

#120019

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mClover3

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mClover3

Use

Pcr template

Tags

mClover3

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-34

Plasmid

#119998

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with 3xFLAG

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

3xFLAG

Use

Pcr template

Tags

3xFLAG

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-15

Plasmid

#119994

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with miRFP670

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

miRFP670

Use

Pcr template

Tags

miRFP670

Promoter

None

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pOPINK-OTUD2 (OTU, aa 132-314)

Plasmid

#61410

Purpose

Expresses human OTUD2 (OTU domain) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUD2 (YOD1 Human)

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-131 and aa 315-348.

Promoter

T7

Available Since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

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pOPINK-OTUD6A (OTU, aa 128-288)

Plasmid

#61417

Purpose

Expresses human OTUD6A (OTU domain) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUD6A (OTUD6A Human)

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-127.

Promoter

T7

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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pOPINK-OTUD6B (OTU, aa 133-293)

Plasmid

#61419

Purpose

Expresses human OTUD6B (OTU domain) in E. coli.

Depositor

David Komander

Article

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

OTUD6B (OTUD6B Human)

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-132.

Promoter

T7

Available Since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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MAC-C SARS-CoV-2 Omicron spike protein

Plasmid

#180778

Purpose

Sars-CoV-2 variant protein

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

MAC-C SARS-CoV-2 Omicron spike protein

Tags

MAC-tag

Expression

Mammalian

Mutation

C64W , S371L, H69-V70-, S373P, S375F, G339D, N211…

Available Since

April 7, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pMaCTag-Z06

Plasmid

#120049

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with sfGFP

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

sfGFP

Use

Pcr template

Tags

sfGFP

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-06

Plasmid

#119985

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with sfGFP

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

sfGFP

Use

Pcr template

Tags

sfGFP

Promoter

None

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pPICZc-Ub-R-HsACTB*-thymosinB-8His

Plasmid

#111149

Purpose

Expresses arginylated human beta-actin fused with thymosin beta and a His tag.

Depositor

Mohan Balasubramanian

Article

Hatano et al J Cell Sci. 2018 Mar 13. pii: jcs.213827

Insert

ACTB (ACTB Human)

Use

Pichia pastoris integration plasmid

Tags

Thymosin beta and a His-tag

Expression

Yeast

Mutation

N-terminal arginylation

Promoter

AOX1

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pMGB

Plasmid

#139932

Purpose

M. magneticum/E. coli shuttle vector (partial sequence of pMGT and p15A)

Depositor

Christopher Voigt

Article

Furubayashi et al Adv. Funct. Mater. 2021, 31, 2004813

Insert

sfGFP

Expression

Bacterial

Promoter

PJ23101

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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small ubiquitin-like modifier 3 (SUMO3)

Plasmid

#221053

Purpose

Expression of small ubiquitin-like modifier 3 (SUMO3) protein under T7 promoter

Depositor

Michael Rosen

Article

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

SUMO 3 (SUMO3 Human)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Jan. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-mNeonGreen-NUT

Plasmid

#204693

Purpose

This plasmid allows for inducible expression of full-length NUT tagged with mNeonGreen, in mammalian cells.

Depositor

Michael Rosen

Article

Kosno et al Sci Rep. 2023 Jul 24;13(1):11907. doi: 1

Insert

NUT (NUTM1 Human)

Tags

mNeonGreen

Expression

Mammalian

Mutation

n/a

Promoter

CMV

Available Since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO-Egr3-EYFP-WPRE

Plasmid

#186417

Purpose

Overexpression of Egr3 transcript variant 1 CDS under EF-1α promoter

Depositor

Mary Kay Lobo

Article

Chandra et al J Neurosci. 2015 May 20;35(20):7927-37.

Insert

Early growth response 3 (Egr3 Mouse)

Use

AAV

Tags

EYFP

Promoter

EF-1-alpha

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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pNL1 Ecarin

Plasmid

#106289

Purpose

Expresses Ecarin in Ecoli off a T7 promoter in the NEB control cloning vector

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

Ecarin

Expression

Bacterial

Promoter

T7

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pVD_85

Plasmid

#149604

Purpose

(parent plasmid pCFB257) pX - 3 -LoxP -KlLEU2 -pTEF1 - XylR

Depositor

Michael Jensen

Article

Ambri et al ACS Synth Biol. 2020 Feb 21;9(2):218-226

Insert

XylR

Use

Synthetic Biology

Tags

SV40

Expression

Yeast

Promoter

TEF1

Available Since

Dec. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pMTTH+mEGFP-LANA-H1.4CTD

Plasmid

#157802

Purpose

Expression of mEGFP, LANA peptide, and human linker histone H1.4 CTD fusion protein

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

mEGFP-LANA-H1.4CTD (H1-4 Human)

Tags

His6 and MBP

Expression

Bacterial

Promoter

pTAC

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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PD-1-miSFIT-1-A, 5-A

Plasmid

#124682

Purpose

Tuning expression of PD-1

Depositor

Tudor Fulga

Article

Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

Insert

PD-1 (PDCD1 Human)

Expression

Mammalian

Promoter

minimal CMV

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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