We narrowed to 15,795 results for: form

1. pLVX-EF1α-BHLHE40 aa1-297-IRES-ZsGreen1

   Plasmid

   #153062

   Purpose

   Lentiviral vector expressing a truncated form of human BHLHE40 (aa 1-297)

   Depositor

   Silvia Monticelli

   Article

   Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

   Insert

   Truncated form of human BHLHE40 (aa 1-297) (BHLHE40 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Mutagenesis to insert a stop codon at aa 298 in B…

   Promoter

   EF1α

   Available since

   July 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. sortase A pentamutant (eSrtA) in pET29

   Plasmid

   #75144

   Purpose

   evolved sortase A (eSrtA) pentamutant with improved kinetics and activity

   Depositor

   David Liu

   Article

   Chen et al Proc Natl Acad Sci U S A. 2011 Jul 12;10

   Insert

   sortase A pentamutant
   

   Use

   Tags

   6x His

   Expression

   Bacterial

   Mutation

   P94R, D160N, D165A, K190E, K196T. Deleted amino a…

   Promoter

   T7

   Available since

   Aug. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET21c(+)-mtLeuRS

   Plasmid

   #31079

   Depositor

   Linda Spremulli

   Article

   Bullard et al Biochim Biophys Acta. 2000 Feb 29. 1490(

   Insert

   Mature form human mitochondrial leucyl-tRNA synthetase (LARS2 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Includes coding sequence for the mature form. Nt …

   Promoter

   Available since

   Sept. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

4. pBz15_Strep_Rubisco

   Plasmid

   #140839

   Purpose

   wild-type Halothiobacillus neapolitanus Form I Rubisco with StrepII tag (CbbL-StrepII, CbbS), pET-14b backbone

   Depositor

   David Savage

   Article

   Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

   Insert

   Form IA Rubisco; CbbL, CbbS
   

   Use

   Tags

   Strep II

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDEST14-SpyCatcher

   Plasmid

   #35044

   Depositor

   Mark Howarth

   Article

   Zakeri et al Proc Natl Acad Sci U S A. 2012 Mar 20;10

   Insert

   SpyCatcher
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Deletion of C-terminal till I113, and point mutat…

   Promoter

   Available since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEGFPC1-GluR1Ctail

   Plasmid

   #32441

   Depositor

   Robert Malinow

   Article

   Rumpel et al Science. 2005 Apr 1;308(5718):83-8. Epub

   Insert

   GluR1 (GRIA1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Contains the last C-terminal 81 amino acids of th…

   Promoter

   CMV

   Available since

   Sept. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. HP138-neo

   Plasmid

   #134247

   Purpose

   Cas9 in a transposon array under a Tet inducible promoter. For making inducible Cas9 cell lines. Neo/G418 selection

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

   Insert

   Contains Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. GFAP-Cre

   Plasmid

   #24704

   Depositor

   Michael Sofroniew

   Article

   Bush et al Neuron. 1999 Jun . 23(2):297-308.

   Insert

   Cre
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   GFAP (glial fibrillary acidic protein)

   Available since

   July 14, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTet-On-TAP

   Plasmid

   #59017

   Purpose

   Construct allows tetracycline inducible expression of a protein of interest fused to a tandem affinity purification tag (TAP) in toxoplasma gondii.

   Depositor

   David Sibley

   Article

   Etheridge et al Cell Host Microbe. 2014 May 14;15(5):537

   Type

   Empty backbone

   Use

   Toxoplasma expression

   Tags

   Calmodulin Binding Peptide fused with a TEV Cleav…

   Expression

   Mutation

   Promoter

   RPS13 with Tet Operators Inserted

   Available since

   Sept. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.0-HA-UbcH13

    Plasmid

    #12461

    Depositor

    Dong-Er Zhang

    Article

    Zou et al Biochem Biophys Res Commun. 2005 Oct 14.

    Insert

    Ubc13 (UBE2N Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET11d_Rev

    Plasmid

    #119322

    Purpose

    Expression of protein Rev from HIV

    Depositor

    Robert Tycko, Francisco Blanco

    Article

    Blanco et al J Mol Biol. 2001 Nov 2;313(4):845-59. do

    Insert

    Rev (rev HIV B1)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGEX-Skp2

    Plasmid

    #19946

    Depositor

    Yue Xiong

    Article

    Michel et al Cell Growth Differ. 1998 Jun . 9(6):435-

    Insert

    S-phase kinase-associated protein 2 (p45) (SKP2 Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. RIP-Timer (DM#285)

    Plasmid

    #15109

    Depositor

    Douglas Melton

    Article

    Dor et al Nature. 2004 May 6. 429(6987):41-6.

    Insert

    insulin II promoter (Ins2 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEGFP-C1-hAtg14

    Plasmid

    #24295

    Depositor

    Noboru Mizushima

    Article

    Itakura et al Mol Biol Cell. 2008 Dec . 19(12):5360-72

    Insert

    autophagy related genes 14 (ATG14 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDEST14-SpyCatcher EQ

    Plasmid

    #35045

    Depositor

    Mark Howarth

    Article

    Zakeri et al Proc Natl Acad Sci U S A. 2012 Mar 20;10

    Insert

    SpyCatcher EQ
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Deletion of C-terminal till I113, and point mutat…

    Promoter

    Available since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pET23 P53DBD

    Plasmid

    #174503

    Purpose

    express P53 DNA binding domain (94-312) in E.coli without tag

    Depositor

    Stewart Loh

    Article

    Butler et al Biochemistry. 2003 Mar 4;42(8):2396-403.

    Insert

    P53 DNA binding domain (corresponds to amino acid 94-312) (TP53 Human)
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBlueRIP.TfrOVA

    Plasmid

    #69596

    Purpose

    contains membrane bound form of ovalbumin (OVA) cDNA in pBlueRIP (used to generate RIP-mOVA mice)

    Depositor

    Francis R. Carbone

    Article

    Kurts et al J Exp Med. 1996 Sep 1;184(3):923-30.

    Insert

    OVA (OVAL Chicken)
    

    Use

    Tags

    Transmembrane domain of Transferrin Receptor (TfR…

    Expression

    Mammalian

    Mutation

    Promoter

    rat insulin promoter (RIP)

    Available since

    Jan. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV-deltaR8.2(A14C,E45C,A92E)

    Plasmid

    #79048

    Purpose

    mammalian expression, lentiviral packaging

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFA-LEU2-I-SceI

    Plasmid

    #67595

    Purpose

    I-SceI expression regulated by tetracycline inducible system in S. pombe

    Depositor

    Kurt Runge

    Article

    Wang et al Mol Cell Biol. 2018 Jul 16;38(15). pii:

    Inserts

    nmt terminator
    I-SceI
    TetR
    

    Use

    Gene targeting in yeast

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MEIS1B-MSCV

    Plasmid

    #21022

    Depositor

    Corey Largman

    Article

    Shen et al Mol Cell Biol. 1999 Apr . 19(4):3051-61.

    Insert

    MEIS1B (Meis1 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 14, 2009

    Availability

    Academic Institutions and Nonprofits only