We narrowed to 23,164 results for: his
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Plasmid#244826PurposeBacterial expression of SpG Cas9 for protein purificationDepositorInsertSpG Cas9
UseCRISPRTags10xHis-MBPExpressionBacterialMutationD1135L/S1136W/G1218K/E1219Q/R1335Q/T1337RPromoterT7Available SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHS24_10xHis_MBP_TEV_SpRY-Cas9
Plasmid#244827PurposeBacterial expression of SpRY Cas9 for protein purificationDepositorInsertSpRY Cas9
UseCRISPRTags10xHis-MBPExpressionBacterialMutationA61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1…PromoterT7Available SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHS25_10xHis_MBP_TEV_EQR-Cas9
Plasmid#244828PurposeBacterial expression of EQR Cas9 for protein purificationDepositorInsertEQR Cas9
UseCRISPRTags10xHis-MBPExpressionBacterialMutationD1135E/R1335Q/T1337RPromoterT7Available SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHS73_10xHis_MBP_TEV_SpRY-Cas9_2xNLS
Plasmid#244835PurposeBacterial expression of SpRY Cas9 with 2xSV40 nuclear localization sequence for protein purificationDepositorInsertSpRY Cas9
UseCRISPRTags10xHis-MBP and 2xSV40 NLSExpressionBacterialMutationA61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1…PromoterT7Available SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHS72_10xHis_MBP_TEV_SpG-Cas9_2xNLS
Plasmid#244834PurposeBacterial expression of SpG Cas9 with 2xSV40 nuclear localization sequence for protein purificationDepositorInsertSpG Cas9
UseCRISPRTags10xHis-MBP and 2xSV40 NLSExpressionBacterialMutationD1135L/S1136W/G1218K/E1219Q/R1335Q/T1337RPromoterT7Available SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a:His-RWT4Ca
Plasmid#237519PurposeRecombinant protein expressionDepositorInsertRwt4Ca
ExpressionBacterialAvailable SinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a:His-MBP-KD
Plasmid#237520PurposeRecombinant protein expressionDepositorInsertRwt4_kinase
ExpressionBacterialMutationKinase region of RWT4Available SinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET15bHis6-pG-Tnp
Plasmid#124884PurposeTo transform BL21-Gold(DE3) and induced by IPTG for His-proteinG-Tnp (hyperactive mutant) fusion protein.DepositorInsertprotein G's C1D1C2D2C3 domains contained in the protein G gBLOCK.
ExpressionBacterialPromoterT7Available SinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
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pML104-HIS3-3
Plasmid#232882PurposePlasmid expressing Cas9 and gRNA AAACCTTTTTACTCCACGCA which targets the HIS3 gene.DepositorAvailable SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML104-HIS3-2
Plasmid#232881PurposePlasmid expressing Cas9 and gRNA CCAAGTTCGACAACTGCGTA which targets the HIS3 gene.DepositorAvailable SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEND-443_Cacnes_HisB
Plasmid#225608PurposeSuicide C. acnes vector to generate HisB (PPA_RS05815) deletion leaving an Ery resistance cassette flanked by BxbI recombination sitesDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
CL7_2xNLS_iGeoCas9(C2)_2xNLS_His
Plasmid#222988PurposeBacterial expression of iGeoCas9(C2) constructDepositorInsertCL7_2xNLS_iGeoCas9(C2)_2xNLS_His
UseCRISPRTagsCL7 and HisExpressionBacterialPromotertacAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
SJZ6-HIS5
Plasmid#228259PurposeTemplate plasmid for yeast gene knockout, uses auxotrophic marker his5 (Schizosaccharomyces Pombe) (complements S.c. his3)DepositorInserthis5
ExpressionYeastPromoterTEF1 S.c.Available SinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSCrhaB2_ApyD_ RBS_ApyAOHIS
Plasmid#226247PurposeProduction of ApyA peptide modified by the B12-rSAM enzyme ApyD (optional, refer to publication), the cytochrome P450 enzyme ApyO, and the MNIO enzyme ApyHI, and the methyltransferase ApySDepositorInsertsHis6_ApyA
ApyD
ApyO
ApyH
ApyI
ApyS
TagsHexa-HistidineExpressionBacterialAvailable SinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(Ct)_3C-TS-His10
Plasmid#223893Purpose4G-cloning donor for C-terminal 3C-TwinSTrep-His10 tagDepositorInsert3C-TwinStrep-His10
UseGolden-gate donorAvailable SinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(Nt)_His6-TEV
Plasmid#223872Purpose4G-cloning donor for N-terminal His6-TEV tagDepositorInsertHis6-TEV
UseGolden-gate donorAvailable SinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(Nt)_His6-FLAG3
Plasmid#223874Purpose4G-cloning donor for N-terminal His6-FLAG3 tagDepositorInsertHis6-FLAG3
UseGolden-gate donorAvailable SinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(Nt)_His6-SUMO
Plasmid#223879Purpose4G-cloning donor for N-terminal His6-SUMO tagDepositorInsertHis6-SUMO
UseGolden-gate donorAvailable SinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(Ct)_3C-His8
Plasmid#223887Purpose4G-cloning donor for C-terminal 3C-His8 tagDepositorInsert3C-His8
UseGolden-gate donorAvailable SinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only