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Showing: 621 - 640 of 1019 results

  1. pML318_SEC23A-N-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid
    #227903
    Purpose
    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
    Depositor
    Manuel Leonetti
    Article
    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.
    Insert
    SEC23A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    227903-plasmid-map-sequence-id-452489

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  2. pML319_SEC31A-N-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid
    #227904
    Purpose
    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
    Depositor
    Manuel Leonetti
    Article
    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.
    Insert
    SEC31A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    227904-plasmid-map-sequence-id-452481

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  3. pML317_COPE-C-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid
    #227905
    Purpose
    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
    Depositor
    Manuel Leonetti
    Article
    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.
    Insert
    COPE homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    227905-plasmid-map-sequence-id-456011

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  5. AICSDP-167:CDH5-mEGFP

    Plasmid
    #217425
    Purpose
    Homology arms and mEGFP sequence for C-terminus tagging of human cadherin 5
    Depositor
    Allen Institute for Cell Science
    Insert
    CDH5 Homology Arms with mEGFP (CDH5 Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP
    Expression
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available since
    Nov. 25, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    217425-plasmid-map-sequence-id-454212

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  6. pcDNA5-FRT-TOgw-C-mEGFP

    Plasmid
    #223953
    Purpose
    Gateway cloning of genes of interest with a C-terminal mEGFP fusion; generation of stable cells with doxycycline inducible expression
    Depositor
    Karla Feijs, Roko Žaja
    Type
    Empty backbone
    Use
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    223953-plasmid-map-sequence-id-450835

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  7. pcDNA5-FRT-TOgw-N-mEGFP

    Plasmid
    #223949
    Purpose
    Gateway cloning of genes of interest with an N-terminal mEGFP fusion; generation of stable cells with doxycycline inducible expression
    Depositor
    Karla Feijs, Roko Žaja
    Type
    Empty backbone
    Use
    Tags
    mEGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    223949-plasmid-map-sequence-id-450830

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  20. pET28-mEGFP(A206K)-TwStrep

    Plasmid
    #208622
    Purpose
    for E. coli expression of C-ter Twin Strep tagged mEGFP(A206K), EGFP cloned from pEGFP-C1, A206K introduced by seamless (Gibson) clone
    Depositor
    Quanfu Ma
    Insert
    mEGFP(A206K)
    Use
    Tags
    6xHis and Twin Strep
    Expression
    Bacterial
    Mutation
    (compared with pEGFP-C1, protein level) A206K
    Promoter
    T7
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208622-plasmid-map-sequence-id-415916

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Showing: 621 - 640 of 1019 results