We narrowed to 1,082 results for: rbis

1. pcDNA3-FLAG_FBXL7

   Plasmid

   #171848

   Purpose

   pcDNA3-FLAG_FBXL7

   Depositor

   Michele Pagano

   Article

   Moro et al Nat Cell Biol. 2020 Sep;22(9):1130-1142.

   Insert

   F-box and leucine rich repeat protein 7 (FBXL7 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. plenti-As-Cas12a-2xNLS

   Plasmid

   #155047

   Purpose

   Lentiviral vector expressing human codon-optimized _C-terminal Myc-tagged _Acidaminococcus sp. BV3L6 (As)-Cas12a nuclease with N- and C-terminal NLS and Neomycin/G418/Geneticin resistance from EF-1alpha promoter

   Depositor

   Jason Moffat, Benjamin Blencowe

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   AsCas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   Nucleoplasmin NLS, Myc-tag and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. FUW-Flag-Mbd3

   Plasmid

   #52371

   Purpose

   Lentiviral expression vector for constitutive expression of double Flag tagged Mbd3

   Depositor

   Jacob Hanna

   Article

   Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

   Insert

   Mbd3 (Mbd3 Mouse)
   

   Use

   Lentiviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   Ub

   Available since

   Sept. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. REV human OCT4 TALEN

   Plasmid

   #52413

   Purpose

   TALEN expressing vector used to allow Knock-in of OCT4-GFP-2A-PURO knockin donor plasmid (generated by Jaenisch lab- Addgene Vector # 31939)

   Depositor

   Jacob Hanna

   Article

   Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

   Insert

   HD HD HD HD HD NI NG NG HD HD NG NI NN NI NI NN NN
   

   Use

   TALEN

   Tags

   Expression

   Mutation

   Promoter

   CAGGS

   Available since

   June 6, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA3.1-2xFLAG-2xSTREP-CDK2

   Plasmid

   #172616

   Purpose

   Expresses 2xFLAG-2xSTREP-tagged CDK2 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CDK2 (CDK2 Human)
   

   Use

   Tags

   2xFLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-eSpCas9-2A-GFP-sgRNA-HSD17B11

   Plasmid

   #161923

   Purpose

   To generate HSD17B11 KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against HSD17B11 exon 1.

   Depositor

   Sébastien Britton

   Article

   Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

   Insert

   sgRNA targeting HSD17B11 exon 1
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pnEA-vH_His-TEV-SEPT2-msfGFP_SEPT6

   Plasmid

   #174498

   Purpose

   bacterial co-expression of human SEPT2 fused to monomeric superfolder GFP and of human SEPT6

   Depositor

   Manos Mavrakis

   Article

   Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

   Inserts

   SEPTIN2 (SEPTIN2 Human)
   SEPTIN6 (SEPTIN6 Human)
   

   Use

   Tags

   His6-TEV and msfGFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. FUW-Mbd3

   Plasmid

   #52356

   Purpose

   Lentiviral expression vector for constitutive expression of Mbd3

   Depositor

   Jacob Hanna

   Article

   Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

   Insert

   Mbd3 (Mbd3 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   Sept. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-2xFLAG-2xSTREP-CDK4

   Plasmid

   #172615

   Purpose

   Expresses 2xFLAG-2xSTREP-tagged CDK4 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CDK4 (CDK4 Human)
   

   Use

   Tags

   2xFLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pnCS_SEPT7_SEPT9_i3-TEV-Strep

    Plasmid

    #174501

    Purpose

    bacterial co-expression of human SEPT7 and of human SEPT9_i3

    Depositor

    Manos Mavrakis

    Article

    Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

    Inserts

    SEPTIN7 (SEPTIN7 Human)
    SEPTIN9 (SEPTIN9 Human)
    

    Use

    Tags

    TEV-Strep

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1-2xFLAG-2xSTREP-CCND3

    Plasmid

    #172623

    Purpose

    Expresses 2xFLAG-2xSTREP-tagged cyclin D3 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND3 (CCND3 Human)
    

    Use

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1-2xFLAG-2xSTREP-CCND2

    Plasmid

    #172629

    Purpose

    Expresses 2xFLAG-2xSTREP-tagged cyclin D2 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND2 (CCND2 Human)
    

    Use

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. FUW-CAGGS-ERAS

    Plasmid

    #52416

    Purpose

    Lentiviral vector encoding constitutive expression of human ERAS cDNA

    Depositor

    Jacob Hanna

    Article

    Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

    Insert

    ERAS (ERAS Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1-CCND1(T286A)

    Plasmid

    #172631

    Purpose

    Expresses untagged cyclin D1(T286A) in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND1 (CCND1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Thr286Ala

    Promoter

    CMV

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. FUW-TetO-lox-ERAS

    Plasmid

    #52417

    Purpose

    Doxycycline inducible lentiviral vector of human ERAS cDNA with excisable insert

    Depositor

    Jacob Hanna

    Article

    Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

    Insert

    ERAS (ERAS Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pB-TR-TTHA

    Plasmid

    #232479

    Purpose

    Tetracycline inducible PiggyBac vector expressing bacterial TTHA1718 (TTHA) gene gene including flag-tag (DYKDDDDK) on the 3' end

    Depositor

    Lukas Trantirek

    Article

    Rynes et al Commun Biol. 2025 Feb 7;8(1):194. doi: 1

    Insert

    Thermus thermophilus HB8
    

    Use

    Transposon system

    Tags

    DYKDDDDK (FLAG-tag)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TetO

    Available since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUC19-U6-[BsmBI_pegRNA_entry]-tevopreQ1-term (LM1138)

    Plasmid

    #223137

    Purpose

    pU6 entry plamid for cloning prime editor tevopreQ1 epegRNAs (requires BsmBI or Esp3I digest and ligation of duplexed oligos)

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    etevopreQ1 entry plasmid backbone for epegRNA expression via human U6 promoter
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-U6-SpCas9_gRNA(RHO-P23H)-CMV-mTagBFP2_(RAS3613)

    Plasmid

    #228252

    Purpose

    pU6 (human U6) expression of SpCas9 sgRNA targeting RHO P23H mutation and pCMV expression of mTagBFP2

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    SpCas9 P23H sgRNA, mTagBFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    CMV and U6

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pUC19-U6-BsmBI_epegRNA_entry-mpknot_(LM1140)

    Plasmid

    #228253

    Purpose

    pU6 entry plamid for cloning prime editor mpknot epegRNAs (requires BsmBI or Esp3I digest and ligation of duplexed oligos)

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    mpknot entry plasmid backbone for epegRNA expression via human U6 promoter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    U6

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pnEA-vH_His-TEV-DSep1-msfGFP

    Plasmid

    #174494

    Purpose

    bacterial expression of Drosophila Sep1 fused to monomeric superfolder GFP

    Depositor

    Manos Mavrakis

    Article

    Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

    Insert

    Sep1 (Sep1 Fly)
    

    Use

    Tags

    His6-TEV and msfGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge