We narrowed to 31,065 results for: LIS;

1. pET21-pelB-LaG94-15

   Plasmid

   #228971

   Purpose

   For bacterial expression of anti-GFP nanobody LaG94-15, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   LaG94-15
   

   Tags

   6xHIS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYPQ-CBE-SpRYn-Act3.0

   Plasmid

   #178958

   Purpose

   It consists of a SpRY nickase (D10A) fused with a cytidine deaminase (A3A/Y130F) and MS2 -SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous C to T conversion and gene activation.

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

   Insert

   hA3A-SpRY-UGI-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-HaloAKAR2.2-T2A-EGFP

   Plasmid

   #216490

   Purpose

   Expression of chemigenetic PKA biosensor (low basal brightness, extremly high dynamic range) and EGFP transfection marker in mammalian cells

   Depositor

   Jin Zhang

   Article

   Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

   Insert

   HaloAKAR2.2
   

   Tags

   T2A-EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. IKK-2 S177E S181E

   Plasmid

   #11105

   Purpose

   Mammalian expression of constitutively active human IKK-2

   Depositor

   Anjana Rao

   Article

   Mercurio et al Science. 1997 Oct 31. 278(5339):860-6.

   Insert

   IKK-2 (IKBKB Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   S177E and S181E, constitutively active

   Available Since

   Feb. 14, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSLQ1658-dCas9-EGFP

   Plasmid

   #51023

   Purpose

   Template for NLS-dCas9-NLS-EGFP fusion protein for CRISPR imaging (the recipient vector can be TetON 3G promoter system)

   Depositor

   Bo Huang, Stanley Qi

   Article

   Chen et al Cell. 2013 Dec 19;155(7):1479-91. doi: 1

   Insert

   dCas9 fuse to EGFP
   

   Use

   CRISPR and Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   MSCV LTR promoter

   Available Since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. MCP-MSN

   Plasmid

   #210704

   Purpose

   This plasmid express EF1a promoter driven MCP-MSN transactivation module

   Depositor

   Isaac Hilton

   Article

   Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

   Insert

   MCP fused to transactivation domain from Human transcription factor MRTF-A, STAT-1 and NRF2
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   N55K in MCP

   Promoter

   EF1a

   Available Since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSLQ1651-sgTelomere(F+E)

   Plasmid

   #51024

   Purpose

   Lentiviral vector that contains an optimized S. pyogenes sgRNA targeting human telomeres

   Depositor

   Bo Huang, Stanley Qi

   Article

   Chen et al Cell. 2013 Dec 19;155(7):1479-91. doi: 1

   Insert

   Optimized sgRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   mouse U6 promoter

   Available Since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. MCP-NMS

   Plasmid

   #210705

   Purpose

   This plasmid express EF1a promoter driven MCP-NMS transactivation module

   Depositor

   Isaac Hilton

   Article

   Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

   Insert

   MCP fused to transactivation domain from Human transcription factor NRF2, MRTF-A and STAT1
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   N55K in MCP

   Promoter

   EF1a

   Available Since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCS2-CRY2-mCherry

   Plasmid

   #140571

   Purpose

   For use in light-induced protein inactivation through the interaction with CIB1

   Depositor

   Heidi Hehnly

   Article

   Rathbun et al Nat Commun. 2020 Mar 9;11(1):1269. doi:

   Insert

   CRY2 (CRY2 Mustard Weed)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV IE94

   Available Since

   Oct. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCCLc-MND-A0201-Mart1-SABR

    Plasmid

    #119052

    Purpose

    3rd gen transfer vector. HLA-A0201 linked to b2-microglobulin and CD28-CD3z signaling domain. Contains the Mart1 epitope "ELAGIGILTV" cloned via BsmBI

    Depositor

    David Baltimore

    Article

    Joglekar et al Nat Methods. 2019 Feb;16(2):191-198. doi

    Insert

    A0201-Mart1-SABR
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    MND

    Available Since

    Jan. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. DOP1B_pcDNA6.2/EmGFP-Bsd

    Plasmid

    #176979

    Purpose

    Mammalian expression vector encoding DOP1B and EmGFP-Bsd

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    DOP1B (DOPEY2 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. VPS13C^mClover3

    Plasmid

    #118760

    Purpose

    VPS13C internally tagged with mClover3 at residue 1914.

    Depositor

    Pietro De Camilli

    Article

    Kumar et al J Cell Biol. 2018 Oct 1;217(10):3625-363

    Insert

    VPS13C (VPS13C Human)
    

    Tags

    mClover3 inserted at position aa 1914

    Expression

    Mammalian

    Available Since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV hUbC-dCas9-MQ1(Q147L)-EGFP

    Plasmid

    #89793

    Purpose

    Targeted CpG methylation

    Depositor

    Margaret Goodell

    Article

    Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

    Insert

    site-specific DNA-methyltransferase SssI
    

    Use

    CRISPR and Lentiviral

    Tags

    3*FLAG and T2A-EGFP

    Expression

    Mammalian

    Mutation

    Q147L, CAA-->CTA

    Promoter

    hUbC

    Available Since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. MCP-eN3x9

    Plasmid

    #210706

    Purpose

    This plasmid express EF1a promoter driven MCP-eN3x9 transactivation module

    Depositor

    Isaac Hilton

    Article

    Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

    Insert

    MCP fused to transactivation domain comprising eNRF2 and 3x 9aa Transactivation domain
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    N55K in MCP

    Promoter

    EF1a

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-HaloAktKAR2.2-T2A-EGFP

    Plasmid

    #216494

    Purpose

    Expression of chemigenetic Akt biosensor (low basal brightness, extremly high dynamic range) and EGFP transfection marker in mammalian cells

    Depositor

    Jin Zhang

    Article

    Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

    Insert

    HaloAktKAR2.2
    

    Tags

    T2A-EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. MCP-3x9aaTAD

    Plasmid

    #210707

    Purpose

    This Plasmid express EF1a promoter driven MCP-3x 9aa transactivation module

    Depositor

    Isaac Hilton

    Article

    Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

    Insert

    MCP fused to 3x 9aa transactivation domain derived from MRTF-B and MYOCD1
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    N55K in MCP

    Promoter

    EF1a

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. scFv-sfGFP-DNMT3A1

    Plasmid

    #102278

    Purpose

    The plasmid encodes a single-chain antibody that binds to the GCN4 peptide from the SunTag system, and is fused to a DNA methyltransferase DNMT3A1

    Depositor

    Margaret Goodell

    Article

    Huang et al Genome Biol. 2017 Sep 18;18(1):176. doi:

    Insert

    DNMT3A1 (DNMT3A Human)
    

    Use

    Lentiviral

    Tags

    scFV-sfGFP

    Expression

    Mammalian

    Available Since

    Dec. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-HaloAKAR2.1-T2A-EGFP

    Plasmid

    #216489

    Purpose

    Expression of chemigenetic PKA biosensor (medium basal brightness, high dynamic range) and EGFP transfection marker in mammalian cells

    Depositor

    Jin Zhang

    Article

    Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

    Insert

    HaloAKAR2.1
    

    Tags

    T2A-EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. HA-HIF1alpha P402A/P564A-pBabe-puro

    Plasmid

    #19005

    Depositor

    William Kaelin

    Article

    Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

    Insert

    Hypoxia inducible factor 1 alpha (HIF1A Human)
    

    Use

    Retroviral

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    cDNA changed amino acids: Proline 402 to Alanin…

    Available Since

    Aug. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBS31-cCARLIN

    Plasmid

    #160928

    Purpose

    Contains 10 constitutively expressed guide RNAs and CAG-GFP-CARLIN array sequence for DNA barcoding (when used in combination with Cas9)

    Depositor

    Fernando Camargo

    Article

    Bowling et al Cell. 2020 Jun 11;181(6):1410-1422.e27.

    Insert

    CARLIN gRNAs and CAG-GFP-CARLIN array
    

    Use

    Mouse Targeting

    Expression

    Mammalian

    Promoter

    U6 promoter for gRNAs; CAG promoter for GFP

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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