We narrowed to 6,524 results for: sas

1. pCAGG-GST-TEV-mCherry-NAP1 (WT)

   Plasmid

   #198036

   Purpose

   Used for the expression and purification of mCherry NAP1 in a mammalian expression system (SMC Internal No. 2065)

   Depositor

   Sascha Martens

   Article

   Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

   Insert

   NAP1 (AZI2 Human)
   

   Tags

   GST-TEV and mCherry

   Expression

   Mammalian

   Available Since

   March 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAGG-GST-TEV-NAP1 (WT)

   Plasmid

   #198034

   Purpose

   Used for the expression and purification of NAP1 in a mammalian expression system (SMC Internal No. 2064)

   Depositor

   Sascha Martens

   Article

   Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

   Insert

   NAP1 (AZI2 Human)
   

   Tags

   GST-TEV

   Expression

   Mammalian

   Available Since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFastBac_Dual_GST-TEV-EGFP-TBK1ΔCTD (res 1-666)

   Plasmid

   #198072

   Purpose

   Used for the expression and purification of EGFP labelled TBK1 carrying a mutation for the removal of the CTD (SMC Internal No. 1874)

   Depositor

   Sascha Martens

   Article

   Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

   Insert

   TBK1 (TBK1 Human)
   

   Tags

   GST-TEV-EGFP

   Expression

   Insect

   Mutation

   TBK1ΔCTD (res 1-666)

   Available Since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGST2-GST-TEV-OPTN S177D S473D

   Plasmid

   #187827

   Purpose

   Plasmid for the expression and purification of GST-TEV-OPTN S177D S473D. Internal Ref: SMC1588

   Depositor

   Sascha Martens

   Article

   Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

   Insert

   OPTN (OPTN Human)
   

   Tags

   GST-TEV

   Expression

   Bacterial

   Mutation

   S177D S473D

   Available Since

   Oct. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-Duet1-6xHis-TEV-NDP52

   Plasmid

   #190194

   Purpose

   Plasmid for the protein expression of 6xHis-TEV-NDP52 in a bacterial expression system. Internal reference: SMC401

   Depositor

   Sascha Martens

   Article

   Zaffagnini et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

   Insert

   NDP52 (CALCOCO2 Human)
   

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Available Since

   Oct. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. nes714Δ1388-1507tk/lacZ

   Plasmid

   #47616

   Purpose

   used to create transgenic mice expressing LacZ reporter with Nestin enhancer

   Depositor

   Urban Lendahl

   Article

   Lothian et al Exp Cell Res. 1999 May 1;248(2):509-19.

   Insert

   nestin 2nd intron fragment (NES Human)
   

   Use

   Enhancer reporter

   Tags

   HSV tk promoter and lacZ

   Mutation

   714 bp from 3' end of 2nd intron with a dele…

   Promoter

   HSV tk promoter

   Available Since

   Aug. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

7. ALDH18A1

   Plasmid

   #155402

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   ALDH18A1 (ALDH18A1 Human)
   

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFastBac_Dual-GST-TEV-SINTBAD-EGFP (codon optimized Sf9)

   Plasmid

   #198035

   Purpose

   Used for the expression and purification of EGFP labelled SINTBAD (SMC Internal No. 1613)

   Depositor

   Sascha Martens

   Article

   Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

   Insert

   SINTBAD (TBKBP1 Human)
   

   Tags

   EGFP and GST-TEV

   Expression

   Insect

   Available Since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLIB_GST-TEV-SRC(WT)

   Plasmid

   #223742

   Purpose

   Expression of recombinant protein for purification, codon optimized for Insect cells

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

   Insert

   Src Kinase (SRC Human)
   

   Tags

   GST-TEV

   Expression

   Insect

   Available Since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pETDuet-1_6xHis-TEV-mCh-NDP52

    Plasmid

    #187829

    Purpose

    Plasmid for the expression and purification of 6xHis-TEV-mCh-NDP52 in E. coli Rosetta pLysS. Internal Reference: SMC1148

    Depositor

    Sascha Martens

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    NDP52 (CALCOCO2 Human)
    

    Tags

    6xHisTag, TEV, and mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-Duet1_6xHis-TEV-eGFP-P62

    Plasmid

    #190929

    Purpose

    Plasmid for the expression and purification of GFP labelled p62. Internal reference: SMC390

    Depositor

    Sascha Martens

    Article

    Turco et al Nat Commun. 2021 Sep 1;12(1):5212. doi:

    Insert

    P62 (SQSTM1 Human)
    

    Tags

    EGFP

    Expression

    Bacterial

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. Tol2 8xSTAR-mNeonGreen. PGK-puro

    Plasmid

    #136255

    Purpose

    Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

    Depositor

    Hugo Snippert

    Article

    Oost et al Cell Rep. 2018 Feb 6;22(6):1600-1614. do

    Insert

    stem cell Ascl2 reporter, 8 repeats
    

    Use

    Tol2 transposase

    Tags

    mNeonGreen

    Expression

    Mammalian

    Available Since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLIB_GST-TEV-SRC (Y530F)

    Plasmid

    #223743

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

    Insert

    Src Kinase (SRC Human)
    

    Tags

    GST-TEV

    Expression

    Insect

    Mutation

    Y530F constitutively active mutant

    Available Since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG_GST-TEV-EGFP-ATG13

    Plasmid

    #223797

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens, James Hurley

    Article

    Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

    Insert

    ATG13 (ATG13 Human)
    

    Tags

    GST-TEV-EGFP

    Expression

    Mammalian

    Available Since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. PB-Neo-TetO-Cdk12_Nterm_FlagHa

    Plasmid

    #127179

    Purpose

    N-terminal Flag- HA- tandem epitope tagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into a dox-inducible piggybac destination vector

    Depositor

    Phil Sharp

    Article

    Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

    Insert

    N-terminally Flag- HA- Epitope Tagged Mouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)
    

    Use

    Piggybac transposase vector

    Tags

    Flag- HA- Tandem Epitopes

    Expression

    Mammalian

    Promoter

    TetO Promoter

    Available Since

    July 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. ReactionC_pBIG1a_ATG7_ATG10

    Plasmid

    #190860

    Purpose

    Plasmid for the expression and purification of ATG7 and ATG10 protein subunit complexes. Internal reference: SMC1179

    Depositor

    Sascha Martens

    Article

    Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

    Inserts

    ATG7 (ATG7 Human)
    ATG10 (ATG10 )
    

    Expression

    Bacterial and Insect

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJH061

    Plasmid

    #243722

    Purpose

    Expresses a chimeric form of ALDH18A1, where the region between residue 97 and 351 is replaced by a 250-residue XTEN linker

    Depositor

    Jonathan Weissman

    Article

    Luo et al Cell. 2025 Aug 25:S0092-8674(25)00916-X.

    Insert

    ALDH18A1(1-97aa)-XTEN250-ALDH18A1(351aa-stop) (ALDH18A1 Human)
    

    Use

    Lentiviral

    Mutation

    The region between residue 97 and 351 of ALDH18A1…

    Available Since

    Oct. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pETDuet_6xHis-FKBP8(1-391aa)-thrombin-GST

    Plasmid

    #227712

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

    Insert

    FKBP8(1-391aa) (FKBP8 Human)
    

    Expression

    Bacterial

    Available Since

    Aug. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pETDuet_6xHis-CCPG1(1-212aa)-thrombin-GST

    Plasmid

    #227713

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

    Insert

    CCPG1(1-212aa) (CCPG1 Human)
    

    Expression

    Bacterial

    Available Since

    Aug. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAG-MBP-OSF-TEVcs-GFP-hULK1

    Plasmid

    #239015

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

    Insert

    hULK1 (ULK1 Human)
    

    Tags

    MBP-OSF-TEV-GFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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