We narrowed to 16,859 results for: por
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pEF1a-T2-NLuc
Plasmid#135938PurposeExpresses NanoLuciferase targeted to the mitochondira in mammalian cells.DepositorInsertNanoLuciferase
UseTagsT2 mitochondrial localization sequenceExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
FLAG-SLC30A10-D248A
Plasmid#82346PurposeExpresses FLAG-tagged SLC30A10-D248A in mammalian cellsDepositorInsertSLC30A10 (SLC30A10 Human)
UseTags3xFLAGExpressionMammalianMutationD248APromoterCMVAvailable sinceSept. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPPC027.306
Plasmid#171149PurposeBiopterin production pathway with J306 scRNA on pBBR1-GmR plasmidDepositorInsertsJ306 scRNA
J3-BBa_J23117-GTPCH_J3-BBa_J23117-PTPS_J3-BBa_J23117-SR
UseCRISPRTagsExpressionBacterialMutationPromoterBBa_J23119 and J3-BBa_J23117Available sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
p.UTA.2.0-GL-2
Plasmid#82440PurposeDual Fluorescent endogenous miRNA sensor. Perfect complementary region of an siRNA control targeting firefly luciferase encoded by the pGL2DepositorInsertLuciferase
UseTagsExpressionMutationThe complementary region for GL2 siRNA control wa…PromoterAvailable sinceMarch 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMP472
Plasmid#134997PurposeInterspersed Reporter. Fluorescent reporter to be used with m6A readers and writers. (8xZF binding sites + 14xGATC sites)-pMinCMV-GFPd2-RbGpA pGK-PuroR-T2A-mCh-BGHpA AAVS1 donorDepositorInsertInters. (8XZFBS 14XGATC)-pMinCMV-GFPd2-RbGpA pGK-PuroR-T2A-mCh-BGHpA AAVS1 donor
UseAAV and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMP498
Plasmid#134998PurposeClustered Reporter. Fluorescent reporter to be used with m6A readers and writers. (5xZF binding sites + 63xGATC sites)-pMinCMV-GFPd2-RbGpA pGK-PuroR-T2A-mCh-BGHpA AAVS1 donorDepositorInsertClust. (5XZFBS 63XGATC)-pMinCMV-GFPd2-RbGpA pGK-PuroR-T2A-mCh-BGHpA AAVS1 donor
UseAAV and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIhh1.3K-Luc
Plasmid#53604PurposeReporter construct containing a 1.3 kb promoter region of the Ihh gene in front of a luciferase gene for in vitro DNA transfection assaysDepositorInsertIhh promoter
UseLuciferase; ReporterTagsExpressionMutationPromoterAvailable sinceJune 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pDGB1a2R+pNOS_Red-F_NOSt
Plasmid#170888PurposeGoldenBraid Transcriptional Unit - pNOS_Red-F_NOSt Reverse orientationDepositorInsertRed-F
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationp.S286Y Red mutantPromoterpNOSAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
TB:2xP53_RE:MiniP
Plasmid#133881Purpose2 copies of the p53 DNA binding motifDepositorInsert2 copies of the p53 DNA binding motif
UseLuciferase and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-EloC
Plasmid#31982DepositorInsertElongin C (ELOC Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pPD3C
Plasmid#86971PurposeExpresses PCNA3 protein fused phosphite dehydrogenase and P450cam in E. coliDepositorInsertPCNA3 fused to phosphite dehydrogenase and P450cam
UseTagsHis6 tagExpressionBacterialMutationE175A/A176R in PTDH, N106R in PCNA3, silent mutat…PromoterT7 promoterAvailable sinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
mCherry-FKBP-TPD54
Plasmid#172451PurposeExpression of Tumor Protein D52 like 2 (TPD54/TPD52L2) with N-terminal mCherry-FKBP tagDepositorInsertTPD54 (TPD52L2 Human)
UseTagsmCherry-FKBPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ERKsubDD-Venus-NES
Plasmid#84630PurposeEncodes C-terminal (substrate) fragment of bimolecular ERK activity reporter (bimEKAR); cytosol targetedDepositorInsertERKsubDD-Venus-NES
UseTags6xHis, Nuclear export signal (NES), T7 tag (gene …ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGREAT4
Plasmid#170892PurposeGoldenBraid Double Transcriptional Unit - pG10-90_E-Luc_NOSt+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…PromoterpG10-90Available sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWZL N-Myc-hPOT1_IRES_hygro
Plasmid#166406Purposeexpress hPOT1 in mammalian cellsDepositorInserthPOT1 (POT1 Human)
UseTagsmycExpressionMammalianMutationPromoterAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pET22b-ecDHFR-Y100F
Plasmid#110826PurposeBacterial expression of E.coli DHFR Y100F mutantDepositorInsertecDHFR
UseTagsExpressionBacterialMutationY100 mutated to F100PromoterT7Available sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only
