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pCDNA5/FRT/TO_CEP41_HaloTag9_T2A_EGFP

Plasmid

#169328

Purpose

Expression of HaloTag9 located at the microtubules of mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

CEP41-HaloTag9 (CEP41 Human)

Tags

HaloTag9

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Available Since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-TGFα-GSY-SPM

Plasmid

#163615

Purpose

Expresses mature human Transforming Growth Factor alpha fused to SPM in bacterial cells for calcium-induced covalent coupling to Epidermal Growth Factor Receptor.

Depositor

Mark Howarth

Article

Scheu et al Nat Commun. 2021 Jan 29;12(1):717. doi:

Insert

TGFa-GSY-SPM (TGFA Synthetic, Human)

Tags

C-tag and His6

Expression

Bacterial

Promoter

T7

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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PLEX-PGK-ANXA11-mCerulean

Plasmid

#164214

Purpose

pLEX lentivirus backbone expresses mCerulean tagged ANXA11 under PGK promoter

Depositor

Jennifer Lippincott-Schwartz

Article

Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

Insert

ANXA11 (ANXA11 Human)

Use

Lentiviral

Tags

mCerulean

Promoter

PGK

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pGWKS9

Plasmid

#139416

Purpose

Fluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised mTurquoise2 marker.

Depositor

James Fraser

Article

Spencer et al Fungal Genet Biol. 2020 May;138:103365.

Insert

mTurquoise2

Expression

Yeast

Promoter

TEF1

Available Since

Oct. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-DIO-GRAB_Ado1.0med

Plasmid

#153292

Purpose

Express the medium-affinity adenosine sensor GRAB_Ado1.0med in Cre positive cells

Depositor

Yulong Li

Article

Wu et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Adenosine sensor GRAB_Ado1.0med (medium affinity)

Use

AAV

Promoter

EFS

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pGWKS5

Plasmid

#139412

Purpose

Fluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised Citrine marker.

Depositor

James Fraser

Article

Spencer et al Fungal Genet Biol. 2020 May;138:103365.

Insert

Citrine

Expression

Yeast

Promoter

TEF1

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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pLC-HA-Nedd8-P2A-Hygro

Plasmid

#124306

Purpose

Lentiviral vector for expression of HA tagged Nedd8-P2A-Hygro casette from a CMV promoter

Depositor

Eva Gottwein

Article

Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

Insert

Nedd8 (NEDD8 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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pCMVtight-RFP-IRES-CD

Plasmid

#126678

Purpose

Doxycycline inducible expression of CD along with reporter RFP

Depositor

Joan Massague

Article

Basnet et al Elife. 2019 Mar 26;8. pii: 43627. doi: 1

Insert

RFP-IRES-CD (FCY1 Budding Yeast)

Promoter

tight TRE

Available Since

June 14, 2019

Availability

Academic Institutions and Nonprofits only

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MS2_adRNA-MCP_ADAR1 DD (E1008Q)_NLS

Plasmid

#124626

Purpose

Expresses MS2_adRNA targeting the RAB7A transcript along with the MCP_ADAR1 DD(E1008Q)_NLS

Depositor

Prashant Mali

Article

Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

Insert

MCP_ADAR1 DD (E1008Q)_NLS

Use

AAV

Tags

NLS

Expression

Mammalian

Mutation

E1008Q hyperactive mutant

Promoter

CMV

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pCF525-EF1a-Hygro-P2A-AcrVA5-lenti

Plasmid

#115664

Purpose

Expresses AcrVA5 in human cells, contained in a lentiviral vector.

Depositor

Jennifer Doudna

Article

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

AcrVA5

Use

Lentiviral

Expression

Bacterial and Mammalian

Promoter

EF-1a promoter

Available Since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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GW1-mito-mRuby2-roGFP1

Plasmid

#107219

Purpose

Mammalian expression plasmid to target the rmRuby1-roGFP1 sensor to the mitochondrial matrix.

Depositor

Mathew Tantama

Article

Norcross et al ACS Sens. 2017 Nov 22;2(11):1721-1729. d

Insert

mRuby2-roGFP1

Tags

4xCox8

Expression

Mammalian

Promoter

CMV

Available Since

March 15, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-P-RAN-H2A2B

Plasmid

#106412

Purpose

P-RAN-H2A2B (RANbody H2A2B::vHRP)

Depositor

Joshua Sanes

Article

Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

Insert

P-RAN-H2A2B

Tags

HA and His

Expression

Mammalian

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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RabV CVS-N2c(deltaG)-APEX2-Venus

Plasmid

#73467

Purpose

Expresses APEX2 fused to Venus for transsynaptic tracing and the EM analysis of infected neurons.

Depositor

Thomas Jessell

Article

Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

Insert

APEX2-Venus

Use

Neurotropic virus

Available Since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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RabV CVS N2c(deltaG)-synaptophysin-GFP

Plasmid

#73492

Purpose

Expresses EGFP for monosynaptic circuit mapping at the synapse

Depositor

Thomas Jessell

Article

Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

Insert

synaptophysin-GFP

Use

Neurotropic virus

Available Since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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pMAL-c5X-Darpin E_01

Plasmid

#66996

Purpose

Expresses malE-Darpin E_01 in E. coli

Depositor

Ashutosh Chilkoti

Article

Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

Insert

malE-Darpin E_01

Tags

His6 and malE-Factor Xa

Expression

Bacterial

Promoter

tac

Available Since

Jan. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pGC629

Plasmid

#71364

Purpose

proximal somatic gonad expression of DAF-16 in C.elegans

Depositor

Jane Hubbard

Article

Qin et al Nat Commun. 2015 May 11;6:7107. doi: 10.

Insert

daf-16 (daf-16 Nematode)

Tags

GFP(65C)

Expression

Worm

Promoter

fos1a promoter

Available Since

Dec. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pSR47

Plasmid

#69258

Purpose

pmyo-3::CRE for integration on ttTi14024, Chr X

Depositor

Sander van den Heuvel

Article

Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

Insert

Cre recombinase

Use

Cre/Lox; Mossci

Expression

Worm

Mutation

codon-optimzed index 1.0

Promoter

myo-3

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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PS-CFP2-H2B-6

Plasmid

#57228

Purpose

Localization: Membrane Adaptor, Excitation: 400 / 490, Emission: 468 / 511

Depositor

Michael Davidson

Insert

H2B (H2BC11 Human)

Tags

PS-CFP2

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

April 1, 2015

Availability

Industry, Academic Institutions, and Nonprofits
DsRed2-H2B-6

Plasmid

#55837

Purpose

Localization: Nucleus/Histones, Excitation: 558, Emission: 583

Depositor

Michael Davidson

Insert

H2B (H2BC11 Human)

Tags

DsRed2

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

March 12, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mPA-GFP-Fibrillarin-7

Plasmid

#57134

Purpose

Localization: Nucleoli, Excitation: 400 / 504, Emission: 515 / 517

Depositor

Michael Davidson

Insert

Fibrillarin (FBL Human)

Tags

mPA-GFP

Expression

Mammalian

Mutation

R36Q, S66F

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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