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Clover-ZO1-C-14

Plasmid

#56375

Purpose

Localization: Tight Junctions, Excitation: 505, Emission: 515

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

ZO1 (TJP1 Human)

Tags

Clover

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2014

Availability

Industry, Academic Institutions, and Nonprofits
mPapaya1-Tubulin-6

Plasmid

#56653

Purpose

Localization: Microtubules, Excitation: 530, Emission: 541

Depositor

Michael Davidson

Article

Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

Insert

Tubulin (TUBA1B Human)

Tags

mPapaya1

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only

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mPapaya1-Rab4a-7

Plasmid

#56652

Purpose

Localization: Ras GTPase, Excitation: 530, Emission: 541

Depositor

Michael Davidson

Article

Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

Insert

Rab4a (RAB4A Human)

Tags

mPapaya1

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only

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mCherry-miniSOG-C-Src-7

Plasmid

#55086

Purpose

Localization: Membrane/Vesicles, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

C-Src

Tags

mCherry-miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only
pMT3-RhoGC

Plasmid

#100031

Purpose

This plasmid is for expression of a rhodopsin/guanylyl cyclase fusion protein.

Depositor

Daniel Oprian

Article

Trieu et al J Biol Chem. 2017 Jun 23;292(25):10379-1

Insert

RhoGC

Tags

1D4 T9'A (AETSQVAPA) and C8 (PRGPDRPEGIEE)

Expression

Mammalian

Promoter

Adenovirus major late promoter

Available Since

Sept. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX Brk-SH2

Plasmid

#46411

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Brk (PTK6 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 74-176

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pCI-Flag-NEDD4L∆WW2

Plasmid

#166708

Purpose

expresses NEDD4L with inactivating mutation in second WW domain in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

Insert

NEDD4L W453A

Tags

FLAG

Expression

Mammalian

Mutation

changed tryptophan 453 to alanine

Promoter

CMV promoter

Available Since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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pCI-Flag-NEDD4L∆WW3

Plasmid

#166709

Purpose

expresses NEDD4L with inactivating mutation in third WW domain in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

Insert

NEDD4L W525A

Tags

FLAG

Expression

Mammalian

Mutation

changed tryptophan 525 to alanine

Promoter

CMV promoter

Available Since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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FnoCas12a-DR-GC-swap-cr in pBCSK+

Plasmid

#126644

Purpose

Cloning vector FnoCas12a-DR-GC-swap-crRNA for expression in mammalian cells

Depositor

Scot Wolfe

Article

Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

Insert

FnoCas12a Full Length Direct Repeat GC Swap crRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6 Promoter

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pET27b-ecDHFR-N23PP/S148A

Plasmid

#110829

Purpose

Bacterial expression of E.coli DHFR N23PP/S148A mutant

Depositor

Stephen Benkovic

Article

Bhabha et al Science. 2011 Apr 8;332(6026):234-8. doi

Insert

ecDHFR

Expression

Bacterial

Mutation

N23 mutated to P23; a proline residue inserted be…

Promoter

T7

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pET51b(+)-NADPH-LUCID-2 (C50 29 nM)

Plasmid

#117909

Purpose

Plasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 29 nM

Depositor

Kai Johnsson

Article

Yu et al Science. 2018 Sep 14;361(6407):1122-1126

Insert

SNAP_Pro30_[cpNLuc23_24]eDHFR(R98A)

Tags

His-tag and Strep-tag

Expression

Bacterial

Mutation

R98A on eDHFR

Promoter

T7

Available Since

Oct. 25, 2018

Availability

Academic Institutions and Nonprofits only

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mEmerald-PCNA-19-NLS-4

Plasmid

#54222

Purpose

Localization: Nucleus, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Lu et al J Cell Biol. 2010 Jun 14;189(6):967-80.

Insert

PCNA-19 NLS (PCNA Human)

Tags

SV40NLS and mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pGEX SHF-SH2

Plasmid

#46491

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

SHF (SHF Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 317-423

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX SHE-SH2

Plasmid

#46490

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

SHE

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 382-492

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX SHD-SH2

Plasmid

#46489

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

SHD (SHD Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 227-340

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX Rin3-SH2

Plasmid

#46476

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Rin3 (RIN3 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 55-157

Promoter

Tac

Available Since

Aug. 1, 2013

Availability

Academic Institutions and Nonprofits only

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mCherry-miniSOG-Paxillin-22

Plasmid

#55092

Purpose

Localization: Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Paxillin

Tags

mCherry-miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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mTFP1-Rab4a-7

Plasmid

#55507

Purpose

Localization: Ras GTPase, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

Rab4a (RAB4A Human)

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

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mCherry-miniSOG-VASP-5

Plasmid

#55098

Purpose

Localization: Actin/Focal Adhesions, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

VASP

Tags

mCherry-miniSOG

Expression

Mammalian

Mutation

A209T in VASP

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only
mCherry-miniSOG-Rab5a-7

Plasmid

#55094

Purpose

Localization: Ras GTPase, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Rab5a

Tags

mCherry-miniSOG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only