We narrowed to 19,574 results for: Cre

1. SBP-mCherry-Ecadherin_puromycin

   Plasmid

   #65293

   Purpose

   Ecadherin reporter only (no hook) (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Ecadherin
   

   Tags

   IL-2 Signal Sequence and Streptavidin Binding Pro…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. FRB-polySH33-EGFP

   Plasmid

   #175792

   Purpose

   Bacterial expression and purification, fluorescently tagged scaffold for droplet visualization and volume quantification

   Depositor

   Michael Rosen

   Article

   Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

   Insert

   NCK1 (NCK1 Human)
   

   Tags

   His6 and MBP

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. YPRCΔ15α1

   Plasmid

   #101712

   Purpose

   GoldenBraid vector pDGB2α1, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YPRCΔ15 solo LTR loci

   Depositor

   Elena Caro Bernat

   Article

   Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

   Type

   Empty backbone

   Use

   Yeast expression

   Available Since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. p3E-rab5c-DN

   Plasmid

   #109737

   Purpose

   Multisite gateway vector for 3' tagging with dominant negative rab5c

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   rab5c (rab5c Zebrafish)
   

   Use

   Zebrafish plasmids

   Mutation

   dominant negative

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. CROP_C9_Puro_JAK2

   Plasmid

   #183303

   Purpose

   All-in-One CRISPRko system with a guide RNA that targets JAK2 gene

   Depositor

   Prashant Mali

   Article

   Hu et al Biomaterials. 2022 Jan;280:121276. doi:

   Insert

   JAK2
   

   Use

   Lentiviral

   Available Since

   May 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. Str-KDEL_flGalT-SBP-tagBFP

   Plasmid

   #65274

   Purpose

   synchronize trafficking of GalT from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   galactosidase
   

   Tags

   Streptavidin Binding Protein (SBP) and tagBFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. ManII-SBP-EGFP_puromycin

   Plasmid

   #65260

   Purpose

   ManII reporter only (no hook) (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   truncated Mannosidae II
   

   Tags

   EGFP and Streptavidin Binding Protein (SBP)

   Expression

   Mammalian

   Mutation

   contains only amino acids 1 to 116 of ManII

   Promoter

   CMV

   Available Since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOCC114

   Plasmid

   #118904

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal sortase site

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-HIS6-TEV-GG-NotI-ccdB-AscI-stop-HindIII cassette
   

   Tags

   HIS6, cleavable with TEV protease, two glycines

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEF-1α_ZF9LOV[C2]-VP64 (pLZA126)

   Plasmid

   #203911

   Purpose

   Encodes engineered opto-ZF9 with AsLOV2(408-543) inserted in C2 position in mammalian cells

   Depositor

   Jared Toettcher

   Article

   Zhu et al Nat Commun. 2023 Jun 2;14(1):3185. doi:

   Insert

   ZF9LOV[C2]-VP64
   

   Expression

   Mammalian

   Promoter

   EF1α

   Available Since

   July 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEF-1α_ZF9LOV[C1]-VP64 (pLZA125)

    Plasmid

    #203910

    Purpose

    Encodes engineered opto-ZF9 with AsLOV2(408-543) inserted in C1 position in mammalian cells

    Depositor

    Jared Toettcher

    Article

    Zhu et al Nat Commun. 2023 Jun 2;14(1):3185. doi:

    Insert

    ZF9LOV[C1]-VP64
    

    Expression

    Mammalian

    Promoter

    EF1α

    Available Since

    July 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDYU1G/mRuby3–histone H1; γ-tubulin-mEGFP

    Plasmid

    #154303

    Purpose

    Expresses mRuby3–histone H1 and γ-tubulin-mEGFP from actin15 promoter in Dictyostelium discoideum

    Depositor

    Shigehiko Yumura

    Article

    Kondo et al Biotechniques. 2020 Feb;68(2):91-95. doi

    Inserts

    histone H1
    γ-tubulin
    

    Use

    Dictyostelium expression

    Tags

    mEGFP and mRuby3

    Promoter

    actin15

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA-Wnt11-V5

    Plasmid

    #35941

    Depositor

    Marian Waterman

    Article

    Najdi et al Differentiation. 2012 Sep;84(2):203-13.

    Insert

    Wnt11 (wnt11f2 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pVenus-Sec16A

    Plasmid

    #36157

    Purpose

    Expresses Sec16A as a Venus FP fusion

    Depositor

    David Stephens

    Article

    Watson et al Traffic. 2006 Dec;7(12):1678-87. Epub 20

    Insert

    Sec16A (SEC16A Human)
    

    Tags

    Venus (EYFP derivative)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA-Wnt9B

    Plasmid

    #35919

    Depositor

    Marian Waterman

    Article

    Najdi et al Differentiation. 2012 Sep;84(2):203-13.

    Insert

    Wnt9B (WNT9B Human)
    

    Expression

    Mammalian

    Mutation

    SNP Met106-->Thr

    Promoter

    CMV

    Available Since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. YPRCΔ15Ω1

    Plasmid

    #101710

    Purpose

    GoldenBraid vector pDGB2Ω1, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YPRCΔ15 solo LTR loci

    Depositor

    Elena Caro Bernat

    Article

    Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

    Type

    Empty backbone

    Use

    Yeast expression

    Available Since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pT3TS-Dest_R1-R3

    Plasmid

    #140878

    Purpose

    166571: Multisite gateway destination vector for in vitro transcription of RNA using T3. Accepts one pME and one p3E entry clone.

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    ccdb/CAM
    

    Expression

    Mammalian

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX-4T-1-p19-T7-PLK1

    Plasmid

    #46312

    Depositor

    Judy Lieberman

    Article

    Huang et al Nat Biotechnol. 2013 Apr;31(4):350-6. do

    Inserts

    p19
    PLK1 DNA-1
    linker
    PLK1 DNA-2
    

    Use

    Pro-sirna

    Tags

    6XHis and GST

    Expression

    Bacterial

    Available Since

    Jan. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pME-AKT-PH

    Plasmid

    #67674

    Purpose

    Gateway entry clone containing AKT-PH with no stop codon

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    AKT-PH
    

    Use

    Gateway multisite middle entry clone

    Available Since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. actc1b-mKate2-CaaX(tH)

    Plasmid

    #109487

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to the CaaX domain from Hras

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    actc1b-mKate2-CaaX(tH)
    

    Use

    Zebrafish plasmids

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pV2A-T

    Plasmid

    #153022

    Purpose

    Single-plasmid multi-gene expression system suitable for fungi. The obtained polycistronic gene is expressed under the control of a TetON inducible promoter

    Depositor

    Vito Valiante

    Article

    Hoefgen et al Metab Eng. 2018 Jul;48:44-51. doi: 10.10

    Type

    Empty backbone

    Expression

    Bacterial and Yeast

    Available Since

    Oct. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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