59,541 results

1. pcDNA3-LacR-TopBP1

   Plasmid

   #31317

   Depositor

   Aziz Sancar

   Article

   Lindsey-Boltz et al J Biol Chem. 2011 Jun 3. 286(22):19229-3

   Insert

   TopBP1 (TOPBP1 Human)
   

   Tags

   FLAG and LacR

   Expression

   Mammalian

   Available Since

   Nov. 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hSyn-RFP

   Plasmid

   #22907

   Depositor

   Edward Callaway

   Article

   Nathanson et al Neuroscience. 2009 Jun 30. 161(2):441-50

   Insert

   human Synapsin I promoter driving DsRedExpress (SYN1 Human)
   

   Use

   AAV; Adeno-associated virus

   Expression

   Mammalian

   Available Since

   April 27, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. mEmerald-Vimentin-N-18

   Plasmid

   #54301

   Purpose

   Localization: Intermediate Filaments, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Insert

   Vimentin (VIM Human)
   

   Tags

   mEmerald

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 16, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

4. GPRC5D-DuET

   Plasmid

   #213298

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   GPRC5D (GPRC5D Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-Myc-ASC

   Plasmid

   #73952

   Purpose

   Express c-Myc-tagged human ASC in mammalian cells

   Depositor

   Christian Stehlik

   Article

   Bryan et al J Immunol. 2009 Mar 1;182(5):3173-82. do

   Insert

   PYD and CARD domain containing, PYCARD (PYCARD Human)
   

   Tags

   c-myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. GFP-2xPH-TAPP1 WT

   Plasmid

   #161990

   Purpose

   Expresses two tandem repeats of Tapp1 PH domain that binds to PI(3,4)P2. Fused to eGFP

   Depositor

   David Bryant

   Article

   Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

   Insert

   PH-TAPP1 (PLEKHA1 Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Available Since

   April 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEM4Z-OMA1

   Plasmid

   #61718

   Purpose

   human Oma1 protease in the E.coli/in vitro translation vector

   Depositor

   Oleh Khalimonchuk

   Insert

   OMA1 (OMA1 Human)
   

   Expression

   Bacterial

   Available Since

   Dec. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pSUMO2

   Plasmid

   #52259

   Purpose

   Bacterial expression of human 6His-tagged SUMO2, SUMO-E1 and SUMO-E2

   Depositor

   Primo Schaer

   Article

   Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

   Inserts

   SUMO-2 (SUMO2 Human)
   UBE2l (UBE2I Human)
   SAE-2 (UBA2 Human)
   SAE-1 (SAE1 Human)
   

   Tags

   6His-tag

   Expression

   Bacterial

   Mutation

   C-terminal gly-gly

   Promoter

   T7 and rbs

   Available Since

   July 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCSC-ASCL1-IRES-GFP-T2A-Sox11

   Plasmid

   #182233

   Purpose

   To convert human skin fibroblasts into induced basal forebrain cholinergic neurons (hiBFCNs) in combination with LHX8, GBX1, FGF2 and two small molecules, forskolin and LDN-193189

   Depositor

   Chun-Li Zhang

   Article

   Ma et al Mol Neurodegener. 2020 Oct 21;15(1):61.

   Insert

   ASCL1-IRES-GFP-T2A-Sox11
   

   Use

   Lentiviral

   Promoter

   CMV

   Available Since

   March 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMXs-puro-RFP-ATG9A

    Plasmid

    #60609

    Purpose

    Expresses RFP-hATG9A in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

    Insert

    Homo sapiens autophagy related 9A (PPARG Human)
    

    Use

    Retroviral

    Tags

    RFP

    Expression

    Mammalian

    Available Since

    Feb. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. PKT2/CLP-AKT

    Plasmid

    #20281

    Depositor

    John Ohlfest

    Article

    Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

    Insert

    AKT (AKT1 Human)
    

    Use

    Transposon

    Available Since

    Dec. 3, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1/CALR wt-FLAG

    Plasmid

    #204532

    Purpose

    Mammalian expression of human CALR wt-FLAG

    Depositor

    Norio Komatsu

    Article

    Araki et al Blood. 2016 Jan 27. pii: blood-2015-09-6

    Insert

    CALR wt-FLAG (CALR Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR221_SLC8A1

    Plasmid

    #132162

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC8A1 (SLC8A1 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 22, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. Myc-PSF-WT

    Plasmid

    #35183

    Depositor

    Benjamin Blencowe

    Article

    Rosonina et al Mol Cell Biol. 2005 Aug;25(15):6734-46.

    Insert

    PSF (SFPQ Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMK382

    Plasmid

    #214373

    Purpose

    Tet-OsTIR1(F74G) (AAVS1 insertion donor)

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    Tet-OsTIR1(F74G)
    

    Expression

    Mammalian

    Promoter

    Tet ON 3G promoter

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. PE-SUMO CRYBB1

    Plasmid

    #217675

    Purpose

    For expression of SUMO tagged human beta B1 crystallin

    Depositor

    Larry David

    Insert

    CRYBB1 (CRYBB1 Human)
    

    Tags

    6XHis and Smt3

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. RNASEL gRNA (BRDN0001149212)

    Plasmid

    #77051

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RNASEL

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    RNASEL (RNASEL Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. RNASEL gRNA (BRDN0001149358)

    Plasmid

    #77052

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human RNASEL

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    RNASEL (RNASEL Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-DRD2-cpHalo∆-mEGFP

    Plasmid

    #194679

    Purpose

    CMV driven expression of D2R GPCR recorder in mammalian cell lines fused to mEGFP

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    DRD2_cpHalo∆
    

    Tags

    mEGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAC150-KEIMA-RAMP4

    Plasmid

    #201929

    Purpose

    PiggyBac Vector expressing KEIMA-RAMP4

    Depositor

    Wade Harper

    Article

    Hoyer et al Nat Cell Biol. 2024 Mar;26(3):378-392. d

    Insert

    RAMP4 (SERP1 Human)
    

    Expression

    Mammalian

    Available Since

    Jan. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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