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pEF5/FRT/DEST-3xHA/mGli2 P1-4A

Plasmid

#51252

Purpose

Encodes N-3xHA-TEV-mouseGli2-C; amino acids 789,805,817,848 mutated to Ala

Depositor

Rajat Rohatgi

Article

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Insert

Gli2 (Gli2 Mouse)

Use

Flpin system

Tags

3xHA-TEV

Expression

Mammalian

Mutation

amino acids 789,805,817,848 mutated to Ala

Promoter

EF1a

Available Since

Feb. 25, 2014

Availability

Academic Institutions and Nonprofits only
pGL2B -688/+64

Plasmid

#24944

Depositor

Stephen Smale

Article

Brightbill et al J Immunol. 2000 Feb 15. 164(4):1940-51.

Insert

IL-10 promoter (Il10 Mouse)

Use

Luciferase

Tags

Luciferase

Available Since

Aug. 18, 2010

Availability

Academic Institutions and Nonprofits only
pISH-Gucy1b2-probe1

Plasmid

#105454

Purpose

to generate a riboprobe for in situ hybridazation

Depositor

Peter Mombaerts

Article

Omura et al Mol Cell Neurosci. 2015 Mar;65:114-24. d

Insert

Gucy1b2 (Gucy1b2 Mouse)

Use

To ganerate riboprobe

Available Since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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Lenti-sgNotch2#2/Cre

Plasmid

#193226

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Notch2 gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgNotch2#2 (Notch2 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 9, 2022

Availability

Academic Institutions and Nonprofits only

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TrpV1 scFv [N221/17]

Plasmid

#182082

Purpose

Mammalian Expression of TrpV1 scFv. Derived from hybridoma N221/17.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting TrpV1 (Rattus norvegicus) (Trpv1 Mouse)

Tags

Sortase, His

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pBabe JunD-Venus puro

Plasmid

#58496

Purpose

mammalian expression of Venus-tagged mouse JunD

Depositor

Kevin Janes

Article

Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

Insert

JunD (Jund Mouse)

Tags

Venus

Expression

Mammalian

Available Since

Aug. 12, 2014

Availability

Academic Institutions and Nonprofits only
pGL3-24p3/LCN2-282kB mut-LUC

Plasmid

#46867

Purpose

murine 24p3 minimal promoter with NF-kB mutation as a luciferase reporter

Depositor

Sarah Gaffen

Article

Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

24p3/lipocalin 2 proximal promoter

Use

Luciferase

Mutation

NF- kB site is mutated (see comments)

Promoter

24p3 promoter (inactive)

Available Since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only
pCMV_FLAG-NFL(K211TAG)

Plasmid

#182660

Purpose

Expresses mouse neurofilament light chain with a TAG codon at the position K211 and an N-terminal FLAG tag (DYKDDDDK) in mammalian cells.

Depositor

Ivana Nikić-Spiegel

Article

Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

Insert

mouse neurofilament light chain with a K211TAG mutation (Nefl Mouse)

Tags

FLAG tag (DYKDDDDK)

Expression

Mammalian

Mutation

K211TAG

Promoter

CMV

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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B108-GFP-IRES-AP

Plasmid

#73988

Purpose

B108 enhancer of Blimp1 gene drives EGFP expression.

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

B108 enhancer

Expression

Mammalian

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pLPCX2-HA-GATA3 C1(259-443)

Plasmid

#89832

Purpose

Express GATA3 C1 in mammalian cells

Depositor

Shengyu Yang

Article

Sun et al J Biol Chem. 2013 Dec 27;288(52):36971-8

Insert

Gata3 (Gata3 Mouse)

Use

Retroviral

Tags

HA

Mutation

C1 (AA 259-443)

Promoter

CMV

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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SRS-MTBD(26/19) AB(monomer), wt

Plasmid

#22381

Depositor

Ron Vale

Article

Gibbons et al J Biol Chem. 2005 Jun 24. 280(25):23960-

Insert

Seryl tRNA synthetase (SRS) – mouse cytoplasmic dynein microtubule binding domain (MTBD) fusion protein

Tags

myc

Expression

Bacterial

Available Since

Nov. 24, 2009

Availability

Academic Institutions and Nonprofits only

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pSR-siKlf5

Plasmid

#41740

Depositor

Jonathan Katz

Article

Yang et al FASEB J. 2007 Feb;21(2):543-50. Epub 200

Insert

Klf5 (Klf5 Mouse)

Use

RNAi

Available Since

Jan. 18, 2013

Availability

Academic Institutions and Nonprofits only
pyc128m

Plasmid

#158778

Purpose

soluble GCaMP expression

Depositor

Jennifer Garrison, Zachary Knight

Article

Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

Insert

GCaMP6m

Use

AAV

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pGL2B -78/+64

Plasmid

#24947

Depositor

Stephen Smale

Article

Brightbill et al J Immunol. 2000 Feb 15. 164(4):1940-51.

Insert

IL-10 promoter (Il10 Mouse)

Use

Luciferase

Tags

Luciferase

Available Since

Aug. 18, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA6-5'UTR(mtIF3)-mtIF3-Dendra2-3'UTR(mtIF3)

Plasmid

#182370

Purpose

Dendra2-based translation reporter

Depositor

Chunghun Lim

Article

Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1

Insert

mtIF3 (Mtif3 Mouse)

Tags

Pal-Dendra2

Expression

Mammalian

Promoter

CMV promoter

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pPB CAG Cdk6 R31C-IRES-EGFP

Plasmid

#153946

Purpose

piggyBac transposon vector with CAG promoter expressing Cdk6 (R31C mutant) and EGFP

Depositor

Luca Tiberi

Article

Ballabio et al Nat Commun. 2020 Jan 29;11(1):583. doi:

Insert

Cdk6 (Cdk6 Mouse)

Expression

Mammalian

Mutation

R31C

Promoter

CAG

Available Since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1(-) TRIM12C

Plasmid

#87080

Purpose

Murine homologues of human Trim5

Depositor

Jeremy Luban

Article

Lascano et al J Virol. 2015 Oct 14;90(1):308-16. doi:

Insert

TRIM12C (Trim12c Mouse)

Expression

Mammalian

Available Since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pv6_MECP2_MBD-R106W

Plasmid

#179408

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MECP2_MBD(R106W)

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

MECP2_MBD(R106W) (Mecp2 Mouse)

Tags

AviTag and EGFP

Expression

Mammalian

Mutation

MBD R106W

Promoter

CAGGS

Available Since

Aug. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR-L1-CAMKII(1.3)-L4

Plasmid

#32577

Depositor

Matthew Nolan

Article

White et al Front Mol Neurosci. 2011;4:8. Epub 2011

Insert

CAMKIIalpha promoter 1.3kb version

Use

Gateway cloning vector

Available Since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

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pCMV10-Flag-Ovtm

Plasmid

#51888

Purpose

expression vectore in mammalian cells

Depositor

Joseph Takahashi

Article

Yoo et al Cell. 2013 Feb 28;152(5):1091-105. doi:

Insert

mFbxl3 (Fbxl3 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

ovtm (I364T)

Promoter

CMV

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only