171,784 results

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mCherry-hLC3B-pcDNA3.1

Plasmid

#40827

Depositor

David Rubinsztein

Article

Jahreiss et al Traffic. 2008 Apr;9(4):574-87. Epub 2008

Insert

LC3B (MAP1LC3B Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-Gcn5

Plasmid

#63706

Purpose

retroviral expression of mouse Gcn5 in mammalian cells

Depositor

Kai Ge

Article

Jin et al EMBO Rep. 2014 Nov;15(11):1192-201. doi:

Insert

Gcn5 (Kat2a Mouse)

Use

Retroviral

Tags

None

Expression

Mammalian

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only
p-dCas9-SSAP-MS2-BB_BbsI

Plasmid

#183826

Purpose

pU6-MS2-gRNA-backbone(BbsI)-CBH-dSpCas9-T2A-EBFP

Depositor

Le Cong

Article

Wang et al Nat Cell Biol. 2022 Feb;24(2):268-278. d

Insert

U6-guideRNA-dCas9-T2A-EBFP

Use

CRISPR

Expression

Mammalian

Promoter

CBH

Available Since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223-PDGFRA

Plasmid

#23892

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PDGFRA (PDGFRA Human)

Use

Gateway donor vector

Mutation

M260I

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pBetaActin-Halo-CAMSAP3

Plasmid

#191329

Purpose

Expresses the fluorescently labeled microtubule minus end marker CAMSAP3 to measure microtubule retrograde flow

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

Halo-CAMSAP3 (Camsap3 Mouse)

Expression

Mammalian

Available Since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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MAC_C_SFXN1

Plasmid

#111676

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

SFXN1 (SFXN1 Human)

Expression

Mammalian

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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ABE8.8-d

Plasmid

#136295

Purpose

expresses ABE8.8-d in mammalian cells

Depositor

Nicole Gaudelli

Article

Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

Insert

ABE8.8-d

Tags

C-terminal BPNLS

Expression

Mammalian

Mutation

D10A in S. pyogenes Cas9, TadA mutations describe…

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pSWEET-bgaB

Plasmid

#210272

Purpose

Xylose-dependent expression system for tight and modulated expression of cloned genes in Bacillus subtilis.

Depositor

Eric Brown

Article

Bhavsar et al Appl Environ Microbiol. 2001 Jan;67(1):4

Insert

bgaB

Expression

Bacterial

Promoter

PxylA

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pET-Sac-Abeta(M1-40)

Plasmid

#71876

Purpose

Expresses the amyloid-beta protein (40 aa), containing an exogenous methionine (Abeta(M1-40)), when expressed in BL21* DE3 p LysS E. coli cells

Depositor

Dominic Walsh

Article

Walsh et al FEBS J. 2009 Mar;276(5):1266-81. doi: 10

Insert

Abeta(M1-40) (APP Human)

Expression

Bacterial

Promoter

T7

Available Since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

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p817-mgammaFcry-EGFP

Plasmid

#23156

Depositor

Zbynek Kozmik

Article

Vopalensky et al Biotechniques. 2010 Mar . 48(3):235-6.

Insert

mouse gammaF crystallin promoter:EGFP (eGFP mouse gammaF promoter:EGFP, Mouse)

Use

Multicloning site flanked by i-scei sites

Mutation

mouse gammaF crystallin promoter (-225 - +73) pre…

Available Since

April 13, 2010

Availability

Academic Institutions and Nonprofits only

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35S-GWY-VP16/pAM

Plasmid

#201232

Purpose

Used to produce and express in planta C-terminal fusion proteins with the VP16 activation domain

Depositor

Michael Ayliffe

Article

Chen et al Plant J. 2023 Jun;114(6):1209-1226. doi:

Insert

35S-GWY-VP16

Expression

Plant

Promoter

CaMV 35S

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-Cepheid1b-ST-WPRE

Plasmid

#214967

Purpose

Red fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter; soma-targeted; for brighter fluorescence

Depositor

Peng Zou

Article

Han et al Sci Adv. 2023 Nov 24;9(47):eadi4208. doi

Insert

Cepheid1b-ST

Use

AAV

Promoter

human Synapsin

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS2708-CMV-Nme2Smu-ABE8e-i1-L10-E932R

Plasmid

#228729

Purpose

Expresses domain inlaid Nme2Smu nCas9 (D16A)-i1-ABE8e base editor with linker-10 and E932R mutation in mammalian cells

Depositor

Erik Sontheimer

Article

Bamidele et al bioRxiv [Preprint]. 2024 Nov 7:2024.10.3

Insert

Domain inlaid Nme2Smu nCas9 (D16A)-i1-L10-E932R

Use

CRISPR

Expression

Mammalian

Mutation

Nme2-Cas9, Smu PID, D16A E932R

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCDH-CMV-Nluc-P2A-copGFP-T2A-Puro

Plasmid

#73037

Purpose

Express Nluc and copGFP dual reporter

Depositor

Kazuhiro Oka

Insert

Nluc-P2A-copGFP-T2A-Puro

Use

Lentiviral and Luciferase

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA-hISG15

Plasmid

#12447

Depositor

Dong-Er Zhang

Article

Kim et al Mol Cell Biol. 2004 Nov . 24(21):9592-60

Insert

ISG15 fragment (ISG15 Human)

Expression

Mammalian

Mutation

ISG15 up to LRLRGG

Available Since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only

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pUC57-Curlcake1

Plasmid

#139340

Purpose

Expresses Curlcake1 (CC1) in E.coli

Depositor

Eva Maria Novoa

Article

Liu et al Nat Commun. 2019 Sep 9;10(1):4079. doi:

Insert

Curlcake1 with T7 promoter

Expression

Bacterial

Promoter

T7

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pUC57-Curlcake2

Plasmid

#139341

Purpose

Expresses Curlcake2 (CC2) in E.coli

Depositor

Eva Maria Novoa

Article

Liu et al Nat Commun. 2019 Sep 9;10(1):4079. doi:

Insert

Curlcake2 with T7 promoter

Expression

Bacterial

Promoter

T7

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pUC57-Curlcake4

Plasmid

#139343

Purpose

Expresses Curlcake4 (CC4) in E.coli

Depositor

Eva Maria Novoa

Article

Liu et al Nat Commun. 2019 Sep 9;10(1):4079. doi:

Insert

Curlcake4 with T7 promoter

Expression

Bacterial

Promoter

T7

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_AktPH-mCherry

Plasmid

#67301

Purpose

Fluorescent indicator for PI(3,4,5)P3

Depositor

Moritoshi Sato

Article

Kawano et al Nat Commun. 2015 Feb 24;6:6256. doi: 10.

Insert

AktPH-mCherry (AKT1 Human, Synthetic)

Expression

Mammalian

Promoter

CMV

Available Since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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pBbE6k-RFP

Plasmid

#35288

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

RFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

LlacO-1

Available Since

Feb. 16, 2012

Availability

Academic Institutions and Nonprofits only

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pTRIP-MND-HsMAN1C1-1-630-cMycFlag-hPGK-GFP

Plasmid

#183505

Purpose

Express GFP and MAN1C1 proteins

Depositor

Olivier Guipaud

Article

Ladaigue et al iScience. 2022 Nov 2;25(12):105482. doi:

Inserts

man1C1 (MAN1C1 Human)
EGFP

Use

Lentiviral

Tags

Myc-DDK

Expression

Mammalian

Promoter

MND and hPGK

Available Since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-hIFITM3

Plasmid

#58397

Purpose

Expressed human IFITM3 with an N-terminal HA tag in mammalian cells

Depositor

Howard Hang, Jacob Yount

Article

Yount et al Nat Chem Biol. 2010 Aug;6(8):610-4. doi:

Insert

IFITM3 (IFITM3 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV IE

Available Since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pHR_5x Gal4 UAS

Plasmid

#79119

Purpose

Base vector for cloning Gal4DBDVP64 inducible genes

Depositor

Wendell Lim

Article

Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

Type

Empty backbone

Use

Lentiviral

Available Since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

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pPB-EF1a-MegaGate-HA-Hygro

Plasmid

#175268

Purpose

MegaGate destination with C-terminal HA tag and hygromycin resistance

Depositor

George Church

Article

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Tags

HA

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pFFLuc-IVT

Plasmid

#205147

Purpose

IVT template of FireFly luciferase stop codon between the ORF and MCS and polyA

Depositor

Igor Ulitsky

Insert

FireFly luciferase

Use

Luciferase; In-vitro transcription

Available Since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CBh-mKate2-IRES-MCS (WT-WT)

Plasmid

#105921

Purpose

AAV-mediated gene expression from CBh promoter. Contains canonical left and right AAV2 ITRs

Depositor

Marcella Patrick

Has Service

AAV2

Inserts

mKate2
IRES-Multiple cloning site

Use

AAV

Mutation

codon optimized

Promoter

CBh and same CBh promoter as mKate2

Available Since

Feb. 15, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3-HA-Sumo1

Plasmid

#21154

Purpose

Mammalian expression of human Sumo1 with HA tag

Depositor

Junying Yuan

Article

Terui et al J Biol Chem. 2004 Jul 2. 279(27):28257-6

Insert

pcDNA3-HA-Sumo1 (SUMO1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

May 11, 2009

Availability

Academic Institutions and Nonprofits only

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His6_MBP_FMRP_P451S

Plasmid

#198693

Purpose

Expression of human FMRP protein in E. coli

Depositor

Simpson Joseph

Article

Edwards et al Sci Rep. 2020 Sep 28;10(1):15858. doi: 1

Insert

FMR1 (FMR1 Human)

Tags

MBP

Expression

Bacterial

Mutation

changed proline 451 to serine

Promoter

T7

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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HaloTag-A2A-adenosine receptor

Plasmid

#66995

Purpose

A2A-adenosine receptor tagged with HaloTag on amino terminus

Depositor

Catherine Berlot

Insert

HaloTag-A2A-adenosine receptor (ADORA2A Human)

Tags

Human Growth hormone Signal Sequence- HaloTag

Expression

Mammalian

Mutation

The signal sequence from human growth hormone was…

Promoter

CMV

Available Since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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pKISox1-P2A-eGFP-Hygro

Plasmid

#115685

Purpose

Knock-in of P2A-eGFP at the mouse Sox1 gene

Depositor

David Suter

Article

Deluz et al Genes Dev. 2016 Nov 15;30(22):2538-2550.

Insert

P2A-eGFP-loxP-PGK-Hygro-loxP

Expression

Mammalian

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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