We narrowed to 13,600 results for: sequence
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Plasmid#214071PurposeLuminescent reporter system to measure EJC-independent NMD activity. No intron in 5'UTRDepositorInsertFirefly luciferase
ExpressionMammalianMutationLinker sequence present between firefly luciferas…Available SinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
ARTX_ctrl
Plasmid#214065PurposeFluorescent reporter system, NMD negative control. No intron in 5'UTRDepositorInsertTurboRFP
ExpressionMammalianMutationRemnant of linker sequence present at the end of …Available SinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGFAP-TetOn3G
Plasmid#133728PurposePlasmid contains the TetOn3G-transactivator sequence under control of a GFAP promoter.DepositorInsertTetOn3G
UseAAVExpressionMammalianPromoterpGFAPAvailable SinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-19:LAMP1-mEGFP
Plasmid#101782PurposeHomology arms and linker-mEGFP sequence for C-terminus tagging of human LAMP1DepositorInsertLAMP1 Homology Arms with linker-mEGFP (LAMP1 Human)
UseCRISPR; Donor templateTagslinker-mEGFPExpressionMammalianAvailable SinceNov. 15, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLenti-Cre-MS2_UbC-NLS-tdMCP-GFP (Cre_tdMCP-GFP)
Plasmid#219537Purposea. Expresses Cre recombinase tagged with 23x repeats of MS2 binding sequence under EF1A promotor and b. NLS-tdMCP_GFP reporter under UbC promotorDepositorAvailable SinceNov. 11, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
ART_RTctrl
Plasmid#214066PurposeFluorescent reporter system, readthrough control, NMD negative control. No intron in 5'UTRDepositorInsertTurboRFP
ExpressionMammalianMutationLinker sequence present between RFP and GFPAvailable SinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-WIPI3
Plasmid#42527DepositorInsertWIPI3 (WDR45B Human)
TagsGST-TEVExpressionMammalianMutationgene sequence is codon optimized for expression i…Available SinceJuly 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pTOX4
Plasmid#127449PurposeEncodes YhaV toxin and amilCP chromoprotein. CAM-RDepositorInsertsyhaV toxin
amilCP chromoprotein
ExpressionBacterialMutationCodon optimized from protein sequence in FPbasePromoterpRha and tacAvailable SinceJuly 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
EasyFusion T2A-H2B-TagBFP
Plasmid#113086PurposeGeneral donor vector with T2A-H2B-TagBFP coding sequence flanking by restriction sites for generating knock-in donorDepositorInsertT2A-H2B-TagBFP
UseSynthetic BiologyAvailable SinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
tetO-MyoD-T2A-GFPnls-mPGK-rtTA-IRES-Puro
Plasmid#69546PurposeTet-On system expressing MyoD-2A-GFP-2xNLS.DepositorInsertsUseLentiviralTags2x nuclear localization sequence and T2A "sk…MutationRemoved stop codon to fuse it to the 2A-eGFPnls i…Available SinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
R57C10-Flp2
Plasmid#63172PurposeR57C10 (N-synaptobrevin), "Pan-Neuronally," driven wild type yeast Flp2 promoter-fusionDepositorInsertyeast wild type containing a Glycine at amino acid 5 of the protein sequence
ExpressionInsectAvailable SinceJuly 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
GFP-eSIBR
Plasmid#71664PurposeEGFP coding region followed by enhanced synthetic inhibitory BIC/miR-155 RNA (eSIBR) backboneDepositorInsertEGFP coding sequence followed by eSIBR cassette
PromoterCMVAvailable SinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDonor_RB-TnV2
Plasmid#213907PurposeBarcoded mariner transposon with kan selection casette, and modified terminal sequences - barcodes can be added via FseI-SbfI restriction sitesDepositorInsertmariner transposon with kan resistance and FseI-SbfI sites for barcode insertion
ExpressionBacterialAvailable SinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTOX5
Plasmid#127450PurposeEncodes MqsR toxin and amilCP chromoprotein. CAM-RDepositorInsertsmqsR toxin
amilCP chromoprotein
ExpressionBacterialMutationCodon optimized from protein sequence in FPbasePromoterpRha and tacAvailable SinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGG-BC-TurboID-GOI
Plasmid#209390PurposeGolden Gate compatible plasmid for TurboID-based proximity labelling experiment in plantaDepositorTypeEmpty backboneExpressionPlantMutationTurboID gene sequence C249AAvailable SinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGG-GOI-DE-TurboID
Plasmid#209388PurposeGolden Gate compatible plasmid for TurboID-based proximity labelling experiment in plantaDepositorTypeEmpty backboneExpressionPlantMutationTurboID gene sequence C249AAvailable SinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMM764
Plasmid#68901PurposeIPTG-inducible CRISPRi vector targeting nonsense sequence (NS), constiutive NanoLuc, pNBU2 backbone, AmpRDepositorInsertsdCas9
LacI
sgRNA
NanoLuc
MutationL51PAvailable SinceOct. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGagMBPHis
Plasmid#42176DepositorInsertGag protein sequence is from HIV-1 clone NL4-3
Tags6xHIS and MBPExpressionBacterialAvailable SinceJuly 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
Plas-gRNA-LEU2
Plasmid#157656PurposeGFP and gRNA tag for protein and mRNA quantification, to attache to the 3' end of the gene coding sequenceDepositorInsertGFP-gRNA
UseInsert storage (replicative in e. coli)TagsGFP-gRNAAvailable SinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
TRF2-IRESS-eGFP
Plasmid#19798DepositorInsertTRF2 (TERF2 Human)
ExpressionMammalianMutationWhen compared to GenBank reference sequence NP_00…PromoterCMV-FAvailable SinceNov. 21, 2008AvailabilityAcademic Institutions and Nonprofits only