We narrowed to 11,618 results for: SHA

1. pETSUMO2_Gillisia-bGSDM

   Plasmid

   #212128

   Purpose

   For expression of the bacterial gasdermin (bGSDM) encoded by a Gillisia species. Protein is expressed with an N-terminal SUMO2 tag.

   Depositor

   Philip Kranzusch

   Article

   Johnson et al Science. 2022 Jan 14;375(6577):221-225.

   Insert

   Gillisia bGSDM
   

   Use

   Tags

   6xHis-SUMO2

   Expression

   Bacterial

   Mutation

   N-terminal methionine deleted and replaced by sin…

   Promoter

   T7

   Available since

   Jan. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. PBLO CasX-gRNA

   Plasmid

   #126419

   Purpose

   E. coli vector for CasX gRNA --- Used for In Vitro Transcription

   Depositor

   Jennifer Doudna

   Article

   Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

   Insert

   casX gRNA
   

   Use

   CRISPR; In vitro transcription

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRGEB-VirD2-Cas9

   Plasmid

   #139803

   Purpose

   Expressing VirD-Cas9 fusion under ubiquitin promoter

   Depositor

   Magdy Mahfouz

   Article

   Ali et al Commun Biol. 2020 Jan 23;3(1):44. doi: 1

   Insert

   VirD2-Cas9
   

   Use

   Tags

   VirD2-Cas9

   Expression

   Plant

   Mutation

   Promoter

   Ubiquitin

   Available since

   April 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. YFP-Rpp21-C1

   Plasmid

   #134542

   Purpose

   Expresses YFP-Rpp21 fusion protein

   Depositor

   Susan Janicki

   Article

   Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

   Insert

   Rpp21 (RPP21 Human)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pENTR-ATXN1

   Plasmid

   #16133

   Depositor

   Huda Zoghbi

   Article

   Lim et al Cell. 2006 May 19. 125(4):801-14.

   Insert

   Ataxin1 (ATXN1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 18, 2008

   Availability

   Academic Institutions and Nonprofits only

6. RSRC1

   Plasmid

   #155983

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   RSRC1 (RSRC1 Human)
   

   Use

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available since

   Sept. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pC1300_pUB10_pco-vCASphi_E9t_V2_pUB10_E9t_ribozyme_AtPDS3_gRNA10

   Plasmid

   #197979

   Purpose

   T-DNA binary vector to express pco-vCasphi driven by UBQ10 gene promoter, and the AtPDS3 guide RNA 10 driven by UBQ10 gene promoter, flanked by ribozymes.

   Depositor

   Steven Jacobsen

   Article

   Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

   Inserts

   pco-vCasphi-2
   AtPDS3 gRNA10
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   pUBQ10

   Available since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. YFP-Rpp14-C1

   Plasmid

   #134540

   Purpose

   Expresses YFP-Rpp14 fusion protein

   Depositor

   Susan Janicki

   Article

   Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

   Insert

   Rpp14 (RPP14 Human)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. YFP-Rpp38-C1

   Plasmid

   #134548

   Purpose

   Expresses YFP-Rpp38 fusion protein

   Depositor

   Susan Janicki

   Article

   Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

   Insert

   Rpp38 (RPP38 Human)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. 6xHis(TEV)-dDrp1-StrepII

    Plasmid

    #174421

    Purpose

    Expresses D. melanogaster Drp1 in bacteria

    Depositor

    Thomas Pucadyil

    Article

    Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

    Insert

    Drp1 (Drp1 Fly)
    

    Use

    Tags

    6xHis(TEV) and StrepII

    Expression

    Bacterial

    Mutation

    None

    Promoter

    T7

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKnockinDonor-humanCTCF-miniAID-mClover3

    Plasmid

    #136883

    Purpose

    knockin donor vector of miniAID-mClover3 to C-terminus of endogenous human CTCF

    Depositor

    Chunliang Li

    Article

    Hyle et al Nucleic Acids Res. 2019 Jul 26;47(13):66

    Insert

    CTCF-knockin-homology arm-miniAID-mClover3
    

    Use

    Tags

    miniAID-mClover3

    Expression

    Mammalian

    Mutation

    Promoter

    no promoter

    Available since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. OA-1067K

    Plasmid

    #164848

    Purpose

    Expresses four gRNA targeting Mhc gene with 3xp3-tdTomato marker

    Depositor

    Omar Akbari

    Article

    Li et al Nat Commun. 2021 Sep 10;12(1):5374. doi:

    Insert

    4 gRNAs and 3xP3-tdTomato
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. OA-874PA

    Plasmid

    #164846

    Purpose

    Expresses AAEL005635-Cas9 with OpIE2-CFP marker

    Depositor

    Omar Akbari

    Article

    Li et al Nat Commun. 2021 Sep 10;12(1):5374. doi:

    Insert

    P10-3'UTR OpIE2-CFP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pXPR_BRD003 sgKEAP1-2

    Plasmid

    #74986

    Purpose

    sgKEAP1-2, puromycin selection

    Depositor

    William Hahn

    Article

    Krall et al Elife. 2017 Feb 1;6:e18970. doi: 10.7554

    Insert

    KEAP1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGMEH-T38M-750-luxAB-DAS+4

    Plasmid

    #49520

    Purpose

    Hygromycin resistant; expresses luxAB-DAS+4 under the control of a promoter containing PtetO-4C5G; constitutively expresses reverse TetR (tetR38); replicates episomally

    Depositor

    Dirk Schnappinger

    Article

    Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

    Insert

    T38M-P750-luxAB-DAS+4
    

    Use

    Tags

    DAS+4 (AANDENYSENYADAS)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. PB-CAG-DD-3xFLAG-hSOX17-GS-TagBFP-BGHpA

    Plasmid

    #172226

    Purpose

    Expression vector with Shield-1 stabilizable hSOX17 fused to TagBFP.

    Depositor

    Alexander Meissner

    Article

    Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

    Insert

    SOX17 (SOX17 Human)
    

    Use

    Piggybac transposon

    Tags

    3XFLAG, DD-domain, GS-linker, and TagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTET2-lucCP

    Plasmid

    #198353

    Purpose

    Part of Renilla reporter system, encodes Firefly luciferase

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    Firefly Luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pTET2

    Available since

    April 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. MSCV FLIPi P2G_Thy1.1 p53

    Plasmid

    #19748

    Depositor

    Richard Hynes

    Article

    Stern et al Proc Natl Acad Sci U S A. 2008 Sep 16. 1

    Insert

    oligo targeting p53 (Trp53 Mouse)
    

    Use

    Cre/Lox, RNAi, and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 18, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. PPAPDC2

    Plasmid

    #155836

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    PPAPDC2 (PLPP6 Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGZ110 (pFA6a-3FLAG-MNase-TRP1)

    Plasmid

    #70233

    Purpose

    ChEC-tagging vector encoding a 3xFLAG tag and aa 83-231 of Mnase in pFA6a-3HA-Trp1 replacing the 3xHA tag. These vectors retain compatibility with the F2/R1 primer pairs commonly used for epitope tag

    Depositor

    Steven Henikoff

    Article

    Zentner et al Nat Commun. 2015 Oct 22;6:8733. doi: 10.

    Insert

    3xFLAG tag and aa 83-231 of Mnase
    

    Use

    Yeast genomic targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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