We narrowed to 11,247 results for: SHA

1. pET15b-Fab1 (Francisella tularensis)

   Plasmid

   #61690

   Purpose

   Inducible expression of the FabI enzyme (enoyl reductase) from Francisella tularensis

   Depositor

   Michael Johnson

   Article

   Hevener et al J Med Chem. 2012 Jan 12;55(1):268-79. do

   Insert

   enoyl-[acyl-carrier-protein] reductase (NADH)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 10, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pFA6a SVEM hphMX6 lys1-I-SceI cleavage site

   Plasmid

   #67648

   Purpose

   I-SceI cleavage site at lys1-hphMX6

   Depositor

   Kurt Runge

   Article

   Wang et al Mol Cell Biol. 2018 Jul 16;38(15). pii:

   Inserts

   lys1 (lys1 Fission Yeast)
   hphMX6
   

   Use

   To test i-scei cleavage efficiency

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRS315-48nt-telo-I-SceI

   Plasmid

   #67623

   Purpose

   telomere cassette for telomere formation system containing 48 bp of S. pombe telomere repeats

   Depositor

   Kurt Runge

   Article

   Wang et al Mol Cell Biol. 2018 Jul 16;38(15). pii:

   Inserts

   3' gal1 unique sequence
   ura4 (ura4 Fission Yeast)
   48 bp telomere repeats
   hph
   

   Use

   Yeast cen/ars plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PGEX-DDX21-D2+C

   Plasmid

   #179243

   Purpose

   protein purification in E. coli

   Depositor

   Ling-Ling Chen

   Article

   Wu et al Science. 2021 Jul 30;373(6554):547-555.

   Insert

   DDX21-D2+C (DDX21 Human)
   

   Use

   Tags

   GST-flag

   Expression

   Bacterial

   Mutation

   aa396-782

   Promoter

   Available since

   March 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFA6a-kanMX6-P41nmt1-GST

   Plasmid

   #39287

   Depositor

   John Pringle, Jurg Bahler

   Article

   Bahler et al Yeast. 1998 Jul;14(10):943-51.

   Insert

   nmt1 promoter (nmt1 Fission Yeast)
   

   Use

   Yeast genomic targeting

   Tags

   A. gossypii translation elongation factor 1a gene…

   Expression

   Mutation

   -1163 to +6 of nmt1 locus with a 4bp deletion (TA…

   Promoter

   Available since

   Sept. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pCaSpeR4 cyto Grx1-roGFP2

   Plasmid

   #64999

   Purpose

   Expression of cytosolic Grx1-roGFP2 under the alphaTub84B promoter

   Depositor

   Tobias Dick

   Article

   Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

   Insert

   Glutaredoxin-1 (GLRX Human)
   

   Use

   Tags

   roGFP2

   Expression

   Insect

   Mutation

   Promoter

   Available since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. R9-WT

   Plasmid

   #125210

   Purpose

   Expressing wildtype human Rad9b gene

   Depositor

   Richard Finnell

   Article

   Cao et al Hum Mutat. 2020 Apr;41(4):786-799. doi:

   Insert

   Rad9b (RAD9B Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLKO.1 Puro shRNA INPP5E KD1

   Plasmid

   #162004

   Purpose

   INPP5E shRNA

   Depositor

   David Bryant

   Article

   Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

   Insert

   INPP5E (INPP5E Canis Lupus)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. 1095=Rcd-1r-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40

   Plasmid

   #159673

   Purpose

   Plasmid supports Cas9 expression in both males and females, Opie2-dsRed tagged, and can be integrated with pBac or phC31.

   Depositor

   Omar Akbari

   Article

   Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

   Insert

   Rcd-1r-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40
   

   Use

   Tags

   T2A-GFP

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3 HRPT2 L64P

    Plasmid

    #11052

    Depositor

    Matthew Meyerson

    Article

    Rozenblatt-Rosen et al Mol Cell Biol. 2005 Jan . 25(2):612-20.

    Insert

    HRPT2 L64P (CDC73 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    L64P

    Promoter

    Available since

    Dec. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

11. pVLAD6-mmPtch1-B-SBP

    Plasmid

    #120886

    Purpose

    BacMam vector for Ptch1-B expression

    Depositor

    Philip Beachy

    Article

    Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

    Insert

    ptch1 (Ptch1 Mouse)
    

    Use

    Tags

    SBP

    Expression

    Mammalian

    Mutation

    deleted amino acids 620-643, truncated at 1291

    Promoter

    CMV

    Available since

    Jan. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. hC73A-pEGFP_N1

    Plasmid

    #111533

    Purpose

    Expresses human KCNB1 C73A in mammalian cells for imaging

    Depositor

    Federico Sesti

    Article

    Yu et al Cell Death Dis. 2017 Apr 6;8(4):e2737. d

    Insert

    human C73A-Kv2.1 (KCNB1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    C73A

    Promoter

    CMV

    Available since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCLXSN(GFP)-HA-Tbkbp1

    Plasmid

    #125177

    Purpose

    Retroviral expression of mouse Tbkbp1

    Depositor

    Shao-Cong Sun

    Article

    Zhu et al Nat Commun. 2018 Jul 18;9(1):2812. doi:

    Insert

    Tbkbp1 (Tbkbp1 Mouse)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA4/TO-myc NES-mCE(K294A) NLS-Flag

    Plasmid

    #82469

    Purpose

    Expresses cytoplasmic restricted form of N-terminal myc tagged and C-terminal flag tagged mRNA capping enzyme, inactive form

    Depositor

    Daniel Schoenberg

    Article

    Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

    Insert

    mRNA capping enzyme (Rngtt Mouse)
    

    Use

    Tags

    Flag and Myc

    Expression

    Mammalian

    Mutation

    changed Lysine 294 to Alanine

    Promoter

    CMV

    Available since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA4/TO-myc NES-mCE NLS-Flag

    Plasmid

    #82468

    Purpose

    Expresses cytoplasmic restricted form of N-terminal myc tagged and C-terminal flag tagged mRNA capping enzyme

    Depositor

    Daniel Schoenberg

    Article

    Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

    Insert

    Myc-NES-mCE-Flag (Rngtt Mouse, HIV)
    

    Use

    Tags

    Flag and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRS315-0nt-telo-I-SceI

    Plasmid

    #67624

    Purpose

    telomere cassette for telomere formation system containing 0 bp of S. pombe telomere repeats

    Depositor

    Kurt Runge

    Article

    Wang et al Mol Cell Biol. 2018 Jul 16;38(15). pii:

    Inserts

    3' gal1 unique sequence
    ura4 (ura4 Fission Yeast)
    telomere cassette and I-SceI cut site
    hph
    

    Use

    Yeast cen/ars plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV10/3xflag-Clock L57E

    Plasmid

    #47337

    Purpose

    Mutation used for co-IPs

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    Clock (Clock Mouse)
    

    Use

    Tags

    3x Flag

    Expression

    Mammalian

    Mutation

    L57E

    Promoter

    CMV

    Available since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pRAN357

    Plasmid

    #120898

    Purpose

    E. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of cyan fluorescent protein (CfpOpt), multiple-cloning site for fusion to N-terminus of target protein

    Depositor

    David S. Weiss, Craig Ellermeier

    Article

    Ransom et al J Bacteriol. 2014 Jun;196(12):2290-300.

    Insert

    cyan fluorescent protein, codon-optimized for low GC organisms, multiple-cloning site for protein fusion
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptet

    Available since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV10/3xflag-Clock L74E

    Plasmid

    #47338

    Purpose

    Mutation used for co-IPs

    Depositor

    Joseph Takahashi

    Article

    Huang et al Science. 2012 Jul 13;337(6091):189-94. d

    Insert

    Clock (Clock Mouse)
    

    Use

    Tags

    3x Flag

    Expression

    Mammalian

    Mutation

    L74E

    Promoter

    CMV

    Available since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pZLCv2-gD4Z4-2-3xFLAG-dCas9-HA-2xNLS

    Plasmid

    #106353

    Purpose

    CRISPR/Cas9 engineered chromatin immunoprecipitation of D4Z4 locus

    Depositor

    Stephen Tapscott

    Article

    Campbell et al Elife. 2018 Mar 13;7. pii: 31023. doi: 1

    Insert

    D4Z4 gRNA-2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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