We narrowed to 14,439 results for: eng
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Plasmid#118800PurposeTo test the effect of sequence on nuclear body formation and splicing of full-length TDP43DepositorInsertTARDBP mutant (TARDBP Human)
UseLentiviralMutationAll FYW to S in CTD of full-length TDP43Available sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBS1412 GFP-TDP43 FYW-L
Plasmid#118801PurposeTo test the effect of sequence on nuclear body formation and splicing of full-length TDP43DepositorInsertTARDBP mutant (TARDBP Human)
UseLentiviralMutationAll FYW to L in CTD of full-length TDP43Available sinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSUPER-hHSF2 shRNA
Plasmid#118346PurposeThis plasmid expresses shRNA against hHSF2.DepositorAvailable sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0112
Plasmid#117548PurposeLevel 1 Golden Gate Cassette: xCas9 3.7 expression cassette for dicotyledonous plantsDepositorInsert2x35S+5'UTR OMEGA (pICH51288) + Sp-xCas9 3.7 (no stop codon) (pEPOR0SP0011) + YFP (pICSL50005) + Nos (pICH41421)
ExpressionPlantPromoter2x35S+5'UTR OMEGA (pICH51288)Available sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-RSV-GFP-U6-EMX1 gRNA (SpyCas9 scaffold)
Plasmid#113040PurposeAAV vector; encodes GFP as well as a U6-driven EMX1-targeting gRNA (SpyCas9 scaffold)DepositorInsertgRNA EMX1 (SpCas9 scaffold), co-expressed GFP (transfection marker)
UseAAV, CRISPR, and Synthetic BiologyExpressionMammalianAvailable sinceNov. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
4xHRE_MLP-sfGFP-CMV_dsRed Exp
Plasmid#117403PurposesfGFP expressed from hypoxia-inducible MLP promoter and dsRed expressed from consitutive CMV promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSuperFolder GFP
ExpressionMammalianAvailable sinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAVPR2_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1
Plasmid#101111PurposeEncodes dCas9(C)-VP64 with SAM components fused to AVPR2DepositorInsertAVPR2-dCas9(C)-VP64
UseCRISPR and Synthetic BiologyTagsFlag and HAExpressionMammalianAvailable sinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLPAR1_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1
Plasmid#101115PurposeEncodes dCas9(C)-VP64 with SAM components fused to LPAR1DepositorInsertLPAR1-dCas9(C)-VP64
UseCRISPR and Synthetic BiologyTagsFlag and HAExpressionMammalianAvailable sinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSUPER-retro-puro-shNup133-1
Plasmid#87334PurposeTo express shRNA against human Nup133DepositorInsertshRNA against human Nup133
UseRNAiExpressionMammalianPromoterH1Available sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSUPER-retro-puro-shNup133-2
Plasmid#87333PurposeTo express shRNA against human Nup133DepositorInsertshRNA against human Nup133
UseRNAiExpressionMammalianPromoterH1Available sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHR-FRB:Akt1(D274A)
Plasmid#86634PurposeExpresses FRB-tagged human Akt1(D274A) kinase-inactive mutantDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFRB-Akt1(D274A) (AKT1 Human)
ExpressionMammalianAvailable sinceApril 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLITMUS-rpoN-cI5G6T,P-J23106-geneIII
Plasmid#80862PurposePhagemid (encodes evolved cI5G6T,P and M13 gene III)DepositorInsertsM13 gene III
cI5G6T,P
ExpressionBacterialPromoterBBa_J23106 and rpoNAvailable sinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLITMUS-rpoN-cI4A5T6T,P-J23106-geneIII
Plasmid#80863PurposePhagemid (encodes evolved cI4A5T6T,P and M13 gene III)DepositorInsertsM13 gene III
cI4A5T6T,P
ExpressionBacterialPromoterBBa_J23106 and rpoNAvailable sinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEGB 35s:dCas:VP64:tNos (GB1189)
Plasmid#75403PurposeTranscriptional unit of (human codon optimized) inactivated Cas9 fused to the VP64 Transcriptional ActivatorDepositorInsertdCas:VP64
UseCRISPR and Synthetic BiologyExpressionPlantPromoter35SAvailable sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
tRNA-gRNA position [n] (GB1207)
Plasmid#75408PurposetRNA and scaffold for the assembly of GBoligomers for the last position (positon [n]) of a polycistronic tRNA-gRNA (2- and 3-part multiplexing)DepositorInserttRNA-gRNA position [n]
UseCRISPR and Synthetic BiologyExpressionPlantAvailable sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
PX381 Filifactor alocis Cas9
Plasmid#68318PurposeFilifactor alocis Cas9DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFaCas9
UseCRISPRTagsHA and NLSExpressionMammalianPromoterCMVAvailable sinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
PX390 Flavobacterium columnare Cas9
Plasmid#68327PurposeFlavobacterium columnare Cas9DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFcCas9
UseCRISPRTagsHA and NLSExpressionMammalianPromoterCMVAvailable sinceAug. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
mCherry_LD 0_CS A_tTA-BFP
Plasmid#58873PurposeMESA target chain with mCherry ectodomain, no linker domain, A cleavage sequence, and tTA-BFP fusionDepositorPublicationInsertMESA target chain with mCherry ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (A in P1')
UseAAVTagsEBFPExpressionMammalianPromoterCMVAvailable sinceNov. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
mCherry_LD 0_CS M_tTA-BFP
Plasmid#58874PurposeMESA target chain with mCherry ectodomain, no linker domain, M cleavage sequence, and tTA-BFP fusionDepositorPublicationInsertMESA target chain with mCherry ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (M in P1')
UseAAVTagsEBFPExpressionMammalianPromoterCMVAvailable sinceSept. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
p14-9VNA5
Plasmid#50931PurposeExpresses human SRP14A5 and SRP9 proteins as a fusion protein in mammalian cells.DepositorTagsVN tag and flag tagExpressionMammalianMutationAmino acids 96-100 of SRP14 changed to alaninePromoterCMVAvailable sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only