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pDONR223_TPK1_p.P152T

Plasmid

#82797

Purpose

Gateway Donor vector containing TPK1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

TPK1 (TPK1 Human)

Use

Gateway Cloning

Mutation

P152T

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_TPK1_p.K111M

Plasmid

#82798

Purpose

Gateway Donor vector containing TPK1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

TPK1 (TPK1 Human)

Use

Gateway Cloning

Mutation

K111M

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_ZBTB24_p.G568C

Plasmid

#82783

Purpose

Gateway Donor vector containing ZBTB24, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

ZBTB24 (ZBTB24 Human)

Use

Gateway Cloning

Mutation

G568C

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PPP2R1A_p.W140C

Plasmid

#82784

Purpose

Gateway Donor vector containing PPP2R1A , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

PPP2R1A (PPP2R1A Human)

Use

Gateway Cloning

Mutation

W140C

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PPP2R1A_p.A541G

Plasmid

#82771

Purpose

Gateway Donor vector containing PPP2R1A , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

PPP2R1A (PPP2R1A Human)

Use

Gateway Cloning

Mutation

A541G

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PIK3R3_p.M295T

Plasmid

#82776

Purpose

Gateway Donor vector containing PIK3R3, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

PIK3R3 (PIK3R3 Human)

Use

Gateway Cloning

Mutation

M295T; D338A

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PIK3R3_p.R245C

Plasmid

#82777

Purpose

Gateway Donor vector containing PIK3R3, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

PIK3R3 (PIK3R3 Human)

Use

Gateway Cloning

Mutation

R245C; D338A

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_ZNF597_p.L283V

Plasmid

#82755

Purpose

Gateway Donor vector containing ZNF597, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

ZNF597 (ZNF597 Human)

Use

Gateway Cloning

Mutation

L283V

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PIK3R3_p.F380L

Plasmid

#82764

Purpose

Gateway Donor vector containing PIK3R3, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

PIK3R3 (PIK3R3 Human)

Use

Gateway Cloning

Mutation

D338A; F380L

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PIK3R3_p.P127L

Plasmid

#82767

Purpose

Gateway Donor vector containing PIK3R3, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

PIK3R3 (PIK3R3 Human)

Use

Gateway Cloning

Mutation

P127L; D338A

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_ZNF597_p.V21L

Plasmid

#82753

Purpose

Gateway Donor vector containing ZNF597, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

ZNF597 (ZNF597 Human)

Use

Gateway Cloning

Mutation

V21L

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_RIT1_p.R122L

Plasmid

#82737

Purpose

Gateway Donor vector containing RIT1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

RIT1 (RIT1 Human)

Use

Gateway Cloning

Mutation

R122L

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_AKR1B1_p.F252L

Plasmid

#82743

Purpose

Gateway Donor vector containing AKR1B1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

AKR1B1 (AKR1B1 Human)

Use

Gateway Cloning

Mutation

F252L

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_STC2_p.R5W

Plasmid

#82744

Purpose

Gateway Donor vector containing STC2, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

STC2 (STC2 Human)

Use

Gateway Cloning

Mutation

R5W

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pTRIPZ-p21-HA

Plasmid

#215110

Purpose

Expresses p21-HA in mammalian cells from an inducible lentiviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

CDKN1A (CDKN1A Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV (Tet-inducible)

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSYN1-NES-OCaMP-WPRE

Plasmid

#224934

Purpose

pAAV (non-flex) for expressing OCaMP, an orange calcium indicator, in mammalian cells and in vivo. Contains hSyn promoter and RSET leader sequence peptide

Depositor

Alexander Lohman

Article

Aggarwal et al bioRxiv 2025.07.28.667269; doi: https://

Insert

OCaMP

Use

AAV

Tags

6xHis

Expression

Mammalian

Promoter

hSynI

Available Since

July 28, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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Tet-pLKO-puro-Scrambled

Plasmid

#47541

Purpose

Tet inducible scrambled shRNA.

Depositor

Charles Rudin

Article

Rudin et al Nat Genet. 2012 Oct;44(10):1111-6. doi:

Insert

Scrambled shRNA

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

H1

Available Since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

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pAAVS1-NDi-CRISPRi (Gen1)

Plasmid

#73497

Purpose

Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus with mCherry marker

Depositor

Bruce Conklin

Article

Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

Inserts

dCas9-KRAB-P2A-mCherry
rtTA

Use

CRISPR

Tags

HA, KRAB, and NLS

Expression

Mammalian

Mutation

D10A, H840A (catalytically deactivated Cas9)

Promoter

CAG and TRE3G

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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rAAV9-CAP-in-cis-Lox-AAV

Plasmid

#205990

Purpose

rAAV9 cap acceptor vector for CREATE libraries

Depositor

Viviana Gradinaru

Article

Chuapoco et al Nat Nanotechnol. 2023 Jul 10. doi: 10.10

Insert

rAAV9 cap acceptor vector for CREATE libraries

Use

AAV

Mutation

Deletion between XbaI and AgeI sites

Available Since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-UbC-Tet1v4-dCas9

Plasmid

#232180

Purpose

Tet1v4-dCas9 from Nuñez et al. 2021, in a lentiviral cassette with blasticidin resistance, for expression in mammalian cells

Depositor

Charles Gersbach

Article

Rohm et al Cell Genom. 2025 Feb 12;5(2):100770. doi

Insert

Tet1 catalytic domain - XTEN80 linker - dead Cas9 - 2A - BlastR (TET1 S. pyogenes, Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

D10A, H840A

Promoter

hUbC

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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