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p-dCas9-SSAP-MS2-BB_BbsI

Plasmid

#183826

Purpose

pU6-MS2-gRNA-backbone(BbsI)-CBH-dSpCas9-T2A-EBFP

Depositor

Le Cong

Article

Wang et al Nat Cell Biol. 2022 Feb;24(2):268-278. d

Insert

U6-guideRNA-dCas9-T2A-EBFP

Use

CRISPR

Expression

Mammalian

Promoter

CBH

Available Since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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pET32a-hEGF

Plasmid

#199231

Purpose

Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

Depositor

Nilson Zanchin

Article

Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

Insert

Epidermal growth factor, partial (EGF Human)

Tags

TrxA-6xHis-S-tag

Expression

Bacterial

Promoter

T7

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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Braf

Plasmid

#40775

Depositor

Dustin Maly

Article

Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

Insert

Braf (BRAF Human)

Tags

FLAG

Expression

Mammalian

Mutation

Starting Met removed

Promoter

CMV

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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Paxillin-pEGFP

Plasmid

#15233

Depositor

Rick Horwitz

Article

Laukaitis et al J Cell Biol. 2001 Jun 25. 153(7):1427-40

Insert

paxillin (PXN Chicken)

Tags

GFP

Expression

Mammalian

Available Since

Sept. 13, 2007

Availability

Academic Institutions and Nonprofits only

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AXBEv2

Plasmid

#204606

Purpose

Mammalian expression of evolved adenine transversion base editor AXBEv2

Depositor

Dali Li

Article

Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103

Insert

bpNLS-TadA-8e-nSpCas9-mAAG (R165E/Y179F)-bpNLS-P2A-GFP

Use

CRISPR

Tags

6*His

Expression

Mammalian

Mutation

mAAG (R165E/Y179F); S. pyogenes Cas9 (D10A)

Available Since

Aug. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-Cas9-P2A-Puro

Plasmid

#110837

Purpose

Lentiviral vector for expression of Cas9 in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9

Use

Lentiviral

Mutation

WT

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pScaf-1512.1

Plasmid

#111402

Purpose

pScaf phagemid with insert for generating DNA origami scaffold 1512 bases in length

Depositor

Shawn Douglas

Article

Nafisi et al Synth Biol (Oxf). 2018 Jan;3(1). doi: 10

Insert

pScaf-1512

Expression

Bacterial

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV MCS mCherry-KASH

Plasmid

#139654

Purpose

Cloning template for AAV-based ORANGE knock-in constructs

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Type

Empty backbone

Use

AAV

Expression

Mammalian

Promoter

U6

Available Since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP Kindlin2

Plasmid

#105305

Purpose

To study effect of Kindlin 2 on Cell/ECM adhesion

Depositor

Kenneth Yamada

Article

Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

Insert

Kindlin2 (FERMT2 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-mCherry-CAAX

Plasmid

#129285

Purpose

Lentiviral plasmid for expression of fluorescent reporter mCherry targeted to the plasma membrane via CAAX polybasic sequence

Depositor

Carlos Carmona-Fontaine

Article

Janska et al Dis Model Mech. 2021 Aug 1;14(8). pii: 2

Insert

Fluorescent reporter mCherry fused to CAAX polybasic sequence

Use

Lentiviral

Tags

CAAX

Expression

Mammalian

Promoter

CMV promoter

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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