We narrowed to 16,749 results for: EMB

1. pGST2-yVps23-UEV_C133A

   Plasmid

   #45924

   Depositor

   James Hurley

   Article

   Ren et al EMBO J. 2011 Jun 1;30(11):2130-9. doi: 1

   Insert

   yeast Vps23-UEV(1-160) (STP22 Budding Yeast)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   C133A

   Promoter

   Available since

   July 29, 2013

   Availability

   Academic Institutions and Nonprofits only

2. HRE-dUnOHR

   Plasmid

   #201714

   Purpose

   Hypoxia responsive promoter driving UnaG and mOrange

   Depositor

   Friedemann Kiefer

   Article

   Erapaneedi et al EMBO J. 2016 Jan 4;35(1):102-13. doi: 10

   Insert

   UnaG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. HRE-dUnaG

   Plasmid

   #201711

   Purpose

   Hypoxia responsive promoter driving destablized UnaG fluorescent protein

   Depositor

   Friedemann Kiefer

   Article

   Erapaneedi et al EMBO J. 2016 Jan 4;35(1):102-13. doi: 10

   Insert

   UnaG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. MscL(G22S)61-cpGFP-ERexp

   Plasmid

   #195311

   Purpose

   Fluorescent reporter/biosensor for membrane tension

   Depositor

   Allen Liu

   Article

   Hsu et al ACS Sens. 2023 Jan 27;8(1):12-18. doi: 1

   Insert

   MscL(G22S)61-cpGFP-ERexp
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Flag Rab12 wild type

   Plasmid

   #86787

   Purpose

   mammalian expression of Rab12

   Depositor

   Peter McPherson

   Article

   Xu et al EMBO Rep. 2015 Jun;16(6):709-18. doi: 10

   Insert

   Rab12 (RAB12 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCAGGS-myrGFP

   Plasmid

   #115502

   Purpose

   expression of myristoylated, plasma membrane targeted GFP

   Depositor

   Thomas Biederer

   Article

   Stagi et al Proc Natl Acad Sci U S A. 2010 Apr 20;10

   Insert

   myristoylated GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   chicken beta-actin

   Available since

   Jan. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

7. MCS+

   Plasmid

   #90412

   Purpose

   A FRET sensor that report the electric potential at the inner leaflet of cell plasma membrane

   Depositor

   Katharina Gaus

   Article

   Ma et al Nat Biotechnol. 2017 Mar 13. doi: 10.103

   Insert

   Lck10+ Venus+mcherry+ strech of positively charged amino acids
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pccGFPG83I

   Plasmid

   #73650

   Purpose

   Fluorescent reporter for transmembrane protein dimerization negative control.

   Depositor

   Alessandro Senes

   Article

   Armstrong et al Biochim Biophys Acta. 2016 Jul 21. pii:

   Insert

   glycophorin A- G83I
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   glycine 83 to isoleucine

   Promoter

   Available since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. BASIC_SEVA_27_pKD46.10

   Plasmid

   #178922

   Purpose

   BASIC DNA assembly vector

   Depositor

   Paul Freemont

   Article

   Haines et al Synth Biol (Oxf). 2022 Oct 11;7(1):ysac0

   Insert

   mScarlet counter-selection cassette
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 7, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. GGE114

    Plasmid

    #120731

    Purpose

    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Destination vector for the insertion of the assembly. It already has some of the parts needed for transformation in Y. lipolytica

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

    Type

    Empty backbone

    Use

    Cloning/assembly plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. TC1154

    Plasmid

    #164876

    Purpose

    Split GFP reporter plasmid expressing GFP (b11beta

    Depositor

    Tian Chi

    Article

    Huang et al EMBO J. 2020 Nov 16;39(22):e104741. doi:

    Insert

    Split GFP(beta11)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMTB

    Plasmid

    #112225

    Purpose

    Expression of membrane-tagged super folder GFP from zebrafish beta-actin promoter.

    Depositor

    Sean Megason

    Article

    Wagner et al Science. 2018 Apr 26. pii: science.aar43

    Insert

    Super-folder GFP
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    beta actin 2 (zebrafish)

    Available since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. p3xFLAG-mSlug

    Plasmid

    #34584

    Depositor

    Celeste Nelson

    Article

    Lee et al EMBO J. 2011 May 24;30(13):2662-74. doi:

    Insert

    Slug (Snai2 Mouse)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

14. p3xFLAG-mSnail

    Plasmid

    #34583

    Depositor

    Celeste Nelson

    Article

    Lee et al EMBO J. 2011 May 24;30(13):2662-74. doi:

    Insert

    Snail (Snai1 Mouse)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pXF89

    Plasmid

    #139030

    Purpose

    MosSCI SapTrap assembly: Cassette 2 for 4- or 5-cassette system

    Depositor

    Erik Griffin

    Article

    Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

    Insert

    halotag (no STOP codon, PATC introns)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJF5

    Plasmid

    #139031

    Purpose

    MosSCI SapTrap assembly: Cassette 2 for 4- or 5-cassette system

    Depositor

    Erik Griffin

    Article

    Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

    Insert

    gfp (no STOP codon, PATC introns)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSDH60

    Plasmid

    #139029

    Purpose

    MosSCI SapTrap assembly: Cassette 1 for 4- or 5-cassette system

    Depositor

    Erik Griffin

    Article

    Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

    Insert

    spe-11 promoter (spe-11 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSM08

    Plasmid

    #139042

    Purpose

    MosSCI SapTrap assembly: Cassette 3 for 4-cassette system

    Depositor

    Erik Griffin

    Article

    Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

    Insert

    mscarlet (includes STOP codon)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPB-CAG-T-V5-GR-2A-H2BmCherry-IP

    Plasmid

    #171500

    Purpose

    Overexpression of T(Brachyury)-GR

    Depositor

    Hans Schöler

    Article

    Aramaki et al EMBO Rep. 2021 Aug 4;22(8):e52553. doi:

    Insert

    T
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA5/FRT/TO-Venus-Flag (865)

    Plasmid

    #40998

    Depositor

    Jonathon Pines

    Article

    Sedgwick et al EMBO J. 2013 Jan 23;32(2):303-14. doi: 1

    Type

    Empty backbone

    Use

    Tags

    3xFLAG (DYKDHDGDYKDHDIDYKDDDDK) and Venus YFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

21. pXF88

    Plasmid

    #139039

    Purpose

    MosSCI SapTrap assembly: Cassette 3 for 4-cassette system

    Depositor

    Erik Griffin

    Article

    Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

    Insert

    halotag (includes STOP codon, PATC introns)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. OmpT

    Plasmid

    #213137

    Purpose

    OmpT_native membrane expression

    Depositor

    Sebastian Hiller

    Article

    Rath et al Nat Commun. 2023 Sep 13;14(1):5648. doi:

    Insert

    OmpT
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. BASIC_SEVA_17_pKD46.10

    Plasmid

    #178917

    Purpose

    BASIC DNA assembly vector

    Depositor

    Paul Freemont

    Article

    Haines et al Synth Biol (Oxf). 2022 Oct 11;7(1):ysac0

    Insert

    mScarlet counter-selection cassette
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 2, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. pJF6

    Plasmid

    #139040

    Purpose

    MosSCI SapTrap assembly: Cassette 3 for 4-cassette system

    Depositor

    Erik Griffin

    Article

    Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

    Insert

    gfp (includes STOP codon, PATC introns)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCMV-TTP(C147R)-GG-PUF

    Plasmid

    #52138

    Purpose

    Receiving vector for PUM-HD assembly and expression in mammalian cells

    Depositor

    Huimin Zhao

    Article

    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.

    Type

    Empty backbone

    Use

    Tags

    (GGGGS)3GGGG linker, 3xFlag, PUM-HD flanking regi…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. PCS2+ Cas9

    Plasmid

    #122948

    Purpose

    For in vitro transcription to produce Cas9 mRNA for microinjection into mouse embryos

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    Cas9
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    SP6

    Available since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSM03

    Plasmid

    #139043

    Purpose

    MosSCI SapTrap assembly: Cassette 3 for 4-cassette system

    Depositor

    Erik Griffin

    Article

    Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

    Insert

    dendra2 (includes STOP codon)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. MCS Lck only

    Plasmid

    #90411

    Purpose

    A control of MCS+ that associated with cell plasma membrane only by one hydrophobic interaction at the N terminus of the FRET pair.

    Depositor

    Katharina Gaus

    Article

    Ma et al Nat Biotechnol. 2017 Mar 13. doi: 10.103

    Insert

    Lck10+ Venus+mcherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDJSVT91

    Plasmid

    #109305

    Purpose

    Express periplasmic, outer membrane, or secreted proteins in E. coli - SufI signal (TAT) and His6 tag

    Depositor

    Daniel Slade

    Article

    Casasanta et al Methods Mol Biol. 2019;1960:123-138. doi

    Type

    Empty backbone

    Use

    Tags

    SufI 1-30, 6His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. MCS Hras FI

    Plasmid

    #90413

    Purpose

    A control of MCS+ that associated with cell plasma membrane by two hydrophobic interaction at N and C terminus of the FRET pair.

    Depositor

    Katharina Gaus

    Article

    Ma et al Nat Biotechnol. 2017 Mar 13. doi: 10.103

    Insert

    Lck10+ Venus+mcherry + H-ras C terminus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. Venus-KRasCT

    Plasmid

    #99016

    Purpose

    Membrane marker

    Depositor

    Narasimhan Gautam

    Article

    O'Neill et al Dev Cell. 2018 Jun 15. pii: S1534-5807(1

    Insert

    Venus KRasCT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CM

    Available since

    June 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. mCherry-KRasCT

    Plasmid

    #99017

    Purpose

    Membrane marker

    Depositor

    Narasimhan Gautam

    Article

    O'Neill et al Dev Cell. 2018 Jun 15. pii: S1534-5807(1

    Insert

    mCherry KRas CT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCZGY2845

    Plasmid

    #87763

    Purpose

    Pnmr-1-PH::miniSOG, expression of membrane targeted PH-miniSOG in subset of interneurons.

    Depositor

    Andrew Chisholm

    Article

    Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

    Insert

    Pnmr-1-PH-miniSOG
    

    Use

    Tags

    PH

    Expression

    Worm

    Mutation

    Promoter

    Pnmr-1

    Available since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCZGY2838

    Plasmid

    #87759

    Purpose

    Pmyo-3-PH::miniSOG, expression of membrane targeted PH-miniSOG in body wall muscles.

    Depositor

    Andrew Chisholm

    Article

    Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

    Insert

    Pmyo-3-PH-miniSOG
    

    Use

    Tags

    PH

    Expression

    Worm

    Mutation

    Promoter

    Pmyo-3

    Available since

    March 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. MAC_C_DUX4

    Plasmid

    #187799

    Purpose

    MAC-tagged gene expression of human DUX4

    Depositor

    Markku Varjosalo

    Article

    Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

    Insert

    DUX4 (DUX4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pUC57-Rluc-BoxB-A114-N40

    Plasmid

    #50562

    Purpose

    Template DNA for in vitro transcription using T7 polymerase

    Depositor

    Yukihide Tomari

    Article

    Fukaya et al EMBO J. 2011 Nov 25;30(24):4998-5009. do

    Insert

    Luciferase-BoxB-A114-N40
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    lac

    Available since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCZGY2842

    Plasmid

    #87762

    Purpose

    Punc-25-PH::miniSOG, expression of membrane targeted PH-miniSOG in GABAergic motor neurons.

    Depositor

    Andrew Chisholm

    Article

    Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

    Insert

    Punc-25-PH-miniSOG
    

    Use

    Tags

    PH

    Expression

    Worm

    Mutation

    Promoter

    Punc-25

    Available since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. lacI-Ptac-lacO-BsaI-BsaI-IMBB4-pUC

    Plasmid

    #170772

    Purpose

    test plasmid for Golden Gate assembly, expresses lacI repressor

    Depositor

    Ichiro Matsumura

    Article

    Matsumura et al Biotechniques. 2022 May;72(5):185-193. d

    Insert

    lacI
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    lacI promoter

    Available since

    Feb. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pMXLK_aFb

    Plasmid

    #114230

    Purpose

    POC12368, MetClo assembly vector with p15A replication origin and kanamycin resistance for BsaI-based MetClo, adaptor sequence type a-b

    Depositor

    Christopher A. O'Callaghan

    Article

    Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

    Insert

    LacZalpha
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pGBW-m4046310

    Plasmid

    #149022

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 M (membrane)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Use

    Tags

    Cleavable thrombin;MBP

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pTEF1

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge