We narrowed to 1,368 results for: Flp
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Plasmid#178514PurposeAAV vector for Flpo recombinase dependent jGCaMP7sDepositorInsertjGCaMP7s
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO/MACROD2
Plasmid#172579PurposeFor generation of FlpIn lines with untagged MACROD2DepositorInsertMACROD2 (MACROD2 Human)
UseTagsnoneExpressionMammalianMutationPromoterCMVAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMVTO-RBDK65E-R89L-nTEVp
Plasmid#179670PurposeMutant Ras Binding Domain split-nTEVPDepositorInsertCRaf RBD protein (RAF1 Human)
UseTagsnTEVPExpressionMammalianMutationK65E, R89LPromoterAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMVTO-RBDK65E-R89L-cTEVp
Plasmid#179671PurposeMutant Ras Binding Domain split-cTEVPDepositorInsertCRaf RBD protein (RAF1 Human)
UseTagscTEVPExpressionMammalianMutationK65E, R89LPromoterAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMVTO-HRAS-G12V
Plasmid#179652PurposeHRAS active mutant (G12V mutation)DepositorInsertHRAS (HRAS Human)
UseTagsExpressionMammalianMutationG12VPromoterAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-LAP-BUB1
Plasmid#115903PurposeUsed for expression of siRNA-resistant BUB1-WT from the FRT site of FlpIn cells upon addition of doxycyclin.DepositorInsertBUB1 (BUB1 Human)
UseTagsEYFPExpressionMammalianMutationPromoterAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-hygro-eGFP-STOP-HEC1
Plasmid#114026PurposeInducible expression of eGFP-tagged HEC1 in FLPin Trex cell lines. Due to insertion of a STOP-codon after the eGFP-tag, this plasmid only expressed eGFP.DepositorInserteGFP-STOP-HEC1 (NDC80 Human)
UseTagsExpressionMammalianMutationStart codon of Hec1 is mutated to stop codonPromoterAvailable sinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Kv4.2LL
Plasmid#114373Purposeexpresses Flpo recombinase-dependent GtACR2-Kv4.2LL, which targets GtACR2 to the somatodendritic compartmentDepositorInsertGtACR2-EYFP-Kv4.2LL (NEWENTRY )
UseAAVTagsEYFP and Kv4.2LLExpressionMammalianMutationPromoterEf1aAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Kv2.1C
Plasmid#114375Purposeexpresses Flpo recombinase-dependent GtACR2-Kv2.1C, which targets GtACR2 to the somatodendritic compartmentDepositorInsertGtACR2-EYFP-Kv2.1C (NEWENTRY )
UseAAVTagsEYFP and Kv2.1CExpressionMammalianMutationPromoterEf1aAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Nlgn1C
Plasmid#114372Purposeexpresses Flpo recombinase-dependent GtACR2-Nlgn1C, which targets GtACR2 to the somatodendritic compartmentDepositorInsertGtACR2-EYFP-Nlgn1C (NEWENTRY )
UseAAVTagsEYFP and Nlgn1CExpressionMammalianMutationPromoterEf1aAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-ARL5A
Plasmid#67378PurposeExpression of native hArl5 in E. coliDepositorInsertARL5A (ARL5A Human)
UseTagsExpressionBacterialMutationbp 70 C to T; silent mutationPromoterT7Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEF5/FRT/DEST-3xHA/mGli2
Plasmid#51250PurposeEncodes N-3xHA-TEV-mouseGli2-CDepositorInsertGli2 (Gli2 Mouse)
UseFlpin systemTags3xHA-TEVExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pK037.CAG-FRT-stop-FRT-RFP-ires-tTA-WPRE (Supernova)
Plasmid#85038PurposeFor Flpe/FRT-based Supernova-RFP, pK037 should be used with pK036.DepositorInsertFRT-stop-FRT
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-ArgiNLS-mGreenLantern
Plasmid#220596PurposeConstitutive expression of a single-cell discriminating version of mGreenLantern fluorescent protein.DepositorInsertmGreenLantern
UseAAVTagsArgiNLSExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-ARL5A-GFP
Plasmid#67399PurposeMammalian expression of hArl5 with C-term GFP tagDepositorInsertARL5A (ARL5A Human)
UseTagsGFPExpressionMammalianMutationbp 70 C to T; silent mutationPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Kv2.1C-TlcnC
Plasmid#114376Purposeexpresses Flpo recombinase-dependent GtACR2-Kv2.1C-TlcnC, which targets GtACR2 to the somatodendritic compartmentDepositorInsertGtACR2-EYFP-Kv2.1C-TlcnC (NEWENTRY )
UseAAVTagsEYFP, Kv2.1C, and TlcnCExpressionMammalianMutationPromoterEf1aAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDSHA-ARL5A-Myc
Plasmid#67357PurposeMammalian expression of hArl5 with C-term Myc tagDepositorInsertARL5A (ARL5A Human)
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
CA-0216 CMVTO-hIL6Ra_M4-rens-hIL6-rens-IL6Rb
Plasmid#231194PurposeMulti-cageIL-6DepositorInserthIL6Ra_M4-rens-hIL6-rens-IL6Rb (IL6 Human)
UseTagsExpressionMammalianMutationA228D, N230D, H280S, D281VPromoterAvailable sinceJan. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
CA-0251 CMVTO-c-myc-mono-hIL10 (R5A11M)-rens
Plasmid#231216Purposemyc-CleaveIL-10DepositorInsertc-myc-mono-hIL10 (R5A11M)-rens (IL10 Human)
UseTagsExpressionMammalianMutationN36I, N110I, K117N, F129LPromoterAvailable sinceJan. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
CA244 CMVTO_ERret-H2M-Renin_T2A_mRens-cageIL-10-c-myc_T2A-mCherry
Plasmid#231205PurposeSingle transcript cageIL-10 (mRens) + ER-renin (H2M)DepositorInsertERret-H2M-Renin_T2A_mRens-cageIL-10-c-myc_T2A-mCherry (IL10 Human)
UseTagsExpressionMammalianMutationL147I, R148H, I203V, L290VPromoterAvailable sinceJan. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
CA-0091 CMVTO-mono-hIL10-R5A11M-rens-3GS-IL10Ra
Plasmid#231191PurposeCageIL-10-monomerDepositorInsertmono-hIL10-R5A11M-rens-3GS-IL10Ra (IL10 Human)
UseTagsExpressionMammalianMutationN36I, N110I, K117N, F129LPromoterAvailable sinceJan. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
CA-0252 CMVTO-c-myc-mono-hIL10 (R5A11M)-rens-cleaved
Plasmid#231215PurposeCleaved-myc-IL-10DepositorInsertc-myc-mono-hIL10 (R5A11M)-rens-cleaved (IL10 Human)
UseTagsExpressionMammalianMutationN36I, N110I, K117N, F129LPromoterAvailable sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
CA173 CMVTO-mono-hIL10-R5A11M-rens-3GS-IL10Ra-c-myc
Plasmid#231214PurposeCageIL-10-mycDepositorInsertmono-hIL10-R5A11M-rens-3GS-IL10Ra-c-myc (IL10 Human)
UseTagsExpressionMammalianMutationN36I, N110I, K117N, F129LPromoterAvailable sinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-ArgiNLS-EGFP
Plasmid#220594PurposeCre-dependent expression of a single-cell discriminating version of EGFP fluorescent protein.DepositorInsertEGFP
UseAAVTagsArgiNLSExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-ArgiNLS-miRFP670
Plasmid#220600PurposeConstitutive expression of a single-cell discriminating version of miRFP670 fluorescent protein.DepositorInsertmiRFP670
UseAAVTagsArgiNLSExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-ArgiNLS-mKate2
Plasmid#220599PurposeConstitutive expression of a single-cell discriminating version of mKate2 fluorescent protein.DepositorInsertmKate2
UseAAVTagsArgiNLSExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-ArgiNLS-AausFP1
Plasmid#220595PurposeConstitutive expression of a single-cell discriminating version of AausFP1 fluorescent protein.DepositorInsertAausFP1
UseAAVTagsArgiNLSExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFORF3030
Plasmid#144506PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertZNF415 (ZNF415 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF2184
Plasmid#141959PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertZNF415 (ZNF415 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF2182
Plasmid#142922PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertZNF415 (ZNF415 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF2183
Plasmid#141958PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertZNF415 (ZNF415 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-ARL5A
Plasmid#67462PurposeMammalian expression of hArl5 with stop codon (native expression)DepositorInsertARL5A (ARL5A Human)
UseTagsExpressionMammalianMutationbp 70 C to T; silent mutationPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-ARL5A-V5-His6
Plasmid#67441PurposeMammalian expression of hArl5 with C-term V5 and His6 tagDepositorInsertARL5A (ARL5A Human)
UseTagsHis and V5ExpressionMammalianMutationbp 70 C to T; silent mutationPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-DEST42-ARL5A-V5-His6
Plasmid#67420PurposeExpression of hArl5 with C-term V5 and His6 tag in E. coliDepositorInsertARL5A (ARL5A Human)
UseTagsHis and V5ExpressionBacterialMutationbp 70 C to T; silent mutationPromoterT7/lacOAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-ARL5A
Plasmid#67324PurposehArl5a with stop codon in Gateway Entry vectorDepositorInsertARL5A (ARL5A Human)
UseGateway entry vectorTagsExpressionBacterialMutationbp 70 C to T; silent mutationPromoterNoneAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-ARL5A-no stop
Plasmid#67325PurposehArl5a without stop codon in Gateway Entry vectorDepositorInsertARL5A (ARL5A Human)
UseGateway entry vectorTagsExpressionBacterialMutationbp 70 C to T; silent mutationPromoterNoneAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEF5/FRT/DEST-3xHA/mGli3/Flag
Plasmid#51246PurposeEncodes N-3xHA-TEV-mouseGli3-Flag-CDepositorInsertGli3 (Gli3 Mouse)
UseFlpin systemTags3xHA-TEV and FlagExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEF5/FRT/DEST-3xHA/mGli2 P1-6A
Plasmid#51251PurposeEncodes N-3xHA-TEV-mouseGli2-C; amino acids 789,805,817,848,923,956 mutated to AlaDepositorInsertGli2 (Gli2 Mouse)
UseFlpin systemTags3xHA-TEVExpressionMammalianMutationamino acids 789,805,817,848,923,956 mutated to AlaPromoterEF1aAvailable sinceFeb. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Kv2.1C-linker-TlcnC
Plasmid#114377Purposeexpresses Flpo recombinase-dependent GtACR2-Kv2.1C-linker-TlcnC, which targets GtACR2 to the somatodendritic compartment. Messier et al found this hybrid construct was the most effective at targetingDepositorInsertGtACR2-EYFP-Kv2.1C-linker-TlcnC (NEWENTRY )
UseAAVTagsEYFP and Kv2.1C-linker-TlcnCExpressionMammalianMutationPromoterEf1aAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCol1a1-frt (FVB)
Plasmid#63575PurposeTargeting vector for the Col1a1 locus that contains a Neo resistance cassette flanked by FRT sites, followed by an ATG-less Hygromycine resistance gene. The homology arms match the sequence of FVB.DepositorInsertCol1A-frt-hygro-pA
UseMouse Targeting; Frt/flpeTagsExpressionBacterial and MammalianMutationPromoterNoneAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only