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pCW57-mCherry-2A-BRD4 Iso C

Plasmid

#137721

Purpose

Doxycycline Inducible Expression of mCherry-2A-FLAG-BRD4 short isoform

Depositor

Scott Floyd

Article

Edwards et al Cell Rep. 2020 Sep 22;32(12):108166. doi

Insert

BRD4 short isoform (BRD4 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Tight TRE promoter

Available since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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SMC2-mAC donor (Hygro)

Plasmid

#140648

Purpose

SMC2 tagging with mAID-Clover

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

SMC2 (SMC2 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-BRD4ΔN-miRFP670-Cry2WT

Plasmid

#122439

Purpose

Expresses disordered protein BRD4(462-1362) fused with fluorescent protein miRFP670 and optogenetic protein Cry2WT

Depositor

Clifford Brangwynne

Article

Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

Insert

BRD4 (BRD4 Human)

Use

Lentiviral

Tags

Cry2WT and miRFP670

Expression

Mammalian

Mutation

Deleted amino acids 1-461

Promoter

SFFV

Available since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-ET

Plasmid

#72542

Purpose

expresses FLAG tagged human BRD4 ET domain (608-699 aa)

Depositor

Christopher Vakoc

Article

Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

Insert

BRD4 ET (BRD4 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pORANGE GFP-CADPS KI

Plasmid

#131482

Purpose

Endogenous tagging of CAPS1: N-terminal (amino acid position: S39)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Cadps Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 and Cbh

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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KHBD00210

Plasmid

#34361

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG17894 (cnc Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pColdI-DDX3_helicase_core

Plasmid

#233618

Purpose

Expression of His-tagged helicase core region of DDX3X in E.coli

Depositor

Shintaro Iwasaki

Article

Chen et al Cell Chem Biol. 2021 Apr 15;28(4):475-48

Insert

DDX3X helicase core WT (DDX3X Human)

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pAPM-miR30-DDX3X-ts3

Plasmid

#174237

Purpose

DDX3X knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

DDX3X shRNA (DDX3X Human)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

SFFV

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAPM-miR30-DDX3X-ts1

Plasmid

#174235

Purpose

DDX3X knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

DDX3X shRNA (DDX3X Human)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

SFFV

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAPM-miR30-DDX3X-ts2

Plasmid

#174236

Purpose

DDX3X knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

DDX3X shRNA (DDX3X Human)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

SFFV

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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sgRNA1_Cas9-hGem_Smc4

Plasmid

#217667

Purpose

expresses Cas9-hGem and guideRNA for tagging of SMC4

Depositor

Daniel Gerlich

Article

Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

Insert

sgRNA1 targeting SMC4 C-terminal (SMC4 Human)

Use

CRISPR

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

U6

Available since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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GB1-A11(2-196, G175R)-Strep

Plasmid

#196211

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, G175R)-Strep.

Depositor

Lalit Deshmukh

Article

Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

Insert

Annexin A11 (ANXA11 Human)

Use

Tags

GB1-6xHis-TEV and Strep-II tag

Expression

Bacterial

Mutation

G175R

Promoter

Available since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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GB1-A11(2-196, G189E)-Strep

Plasmid

#196212

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, G189E)-Strep.

Depositor

Lalit Deshmukh

Article

Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

Insert

Annexin A11 (ANXA11 Human)

Use

Tags

GB1-6xHis-TEV and Strep-II tag

Expression

Bacterial

Mutation

G189E

Promoter

Available since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA-ET-F656W

Plasmid

#72546

Purpose

expresses FLAG tagged human BRD4 ET domain (608-699 aa) with F656W mutation

Depositor

Christopher Vakoc

Article

Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

Insert

BRD4 ET (BRD4 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

F656W

Promoter

Available since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA-ET-I654Q

Plasmid

#72545

Purpose

expresses FLAG tagged human BRD4 ET domain (608-699 aa) with I654Q mutation

Depositor

Christopher Vakoc

Article

Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

Insert

BRD4 ET (BRD4 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

I654Q

Promoter

Available since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA-ET-L630W

Plasmid

#72543

Purpose

expresses FLAG tagged human BRD4 ET domain (608-699 aa) with L630W mutation

Depositor

Christopher Vakoc

Article

Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

Insert

BRD4 ET (BRD4 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

L630W

Promoter

Available since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

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IsHSP70-mCherry

Plasmid

#220092

Purpose

Expresses in ISE6 (Ixodes scapularis) cells, heat inducible. Plasmid backbone derived from pGL-4.79 [Rluc] (Promega)

Depositor

Monika Gulia-Nuss

Article

Pham et al iScience. 2024 Jul 8;27(8):110468. doi:

Insert

HSP70 promoter from Ixodes scapularis

Use

Tags

Expression

Mutation

Promoter

IsHSP70

Available since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pAR-B1(1986.1)

Plasmid

#78503

Purpose

Trans plasmid for production of recombinant AAV-B1 vectors. Plasmid encodes AAV2 Rep and AAV-B1 capsid

Depositor

Miguel Sena-Esteves

Article

Choudhury et al Mol Ther. 2016 Apr 27. doi: 10.1038/mt.2

Inserts

AAV2 Rep
AAV-B1 capsid

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 1, 2016

Availability

Academic Institutions and Nonprofits only

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pONSY-Lifeact:mCherry

Plasmid

#111876

Purpose

This plasmid expresses mCherry fused to Lifeact peptide. It labels actin bundles and filopodia in Capsaspora. Codons have been optimised according to their usages in Capsaspora and C. fragrantissima.

Depositor

Inaki Ruiz-Trillo

Article

Parra-Acero et al Development. 2018 May 11. pii: dev.16210

Insert

Lifeact fused to mCherry

Use

Capsaspora owczarzaki

Tags

mCherry

Expression

Mutation

Promoter

Elongation Factor 1 alpha (EF1a) from Capsaspora

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-FLAG-Co-NF-kB

Plasmid

#180197

Purpose

Expresses FLAG-tagged Capsaspora NF-kB protein in mammalian cells

Depositor

Thomas Gilmore

Article

Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

Insert

Co-NF-kB

Use

Tags

FLAG-tagged fusion protein

Expression

Mammalian

Mutation

aa 2-1224

Promoter

CMV

Available since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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